Harmonia axyridis (Pallas, 1773)

Castro-Guedes, Camila Fediuk de, Almeida, Lúcia Massutti de, Penteado, Susete do Rocio Chiarello & Moura, Mauricio Osvaldo, 2016, Effect of different diets on biology, reproductive variables and life and fertility tables of Harmonia axyridis (Pallas) (Coleoptera, Coccinellidae), Revista Brasileira de Entomologia 60 (3), pp. 260-266 : 261

Harmonia axyridis

For creating the stock, H. axyridis adults were collected on pine branches in October 2011 in Curitiba , Brazil and raised in plastic containers of 500 ml in brood chambers 25 ± 1 ◦ C, 70 ± 10% RH and 12:12 h L: D. The adults were sexed according to the method of McCornack et al. (2007) and kept in pairs for obtaining eggs, which were later transferred to Petri dishes with moist filter paper. After hatching, the larvae were individually placed in Petri dishes lined with filter paper and a cotton swab moistened with a drop of honey. To maintain the stock population, three diets were given simultaneously (so as not to induce preference for the type of food in the daily experiments) ad libitum and once daily. From the formation of prepupae until the emergence of adults, insects were kept under the same conditions described above, with no food. Third generation individuals were used for the bioassays .