Kodamaea yamadae C.Y. Chai & F.L. Hui, 2022

Chai, Chun-Yue, Gao, Wan-Li, Li, Ying, Yan, Zhen-Li & Hui, Feng-Li, 2022, Kodamaea hongheensis f. a., sp. nov., Kodamaea ovata f. a., sp. nov. and Kodamaea yamadae f. a., sp. nov., three new yeast species of Kodamaea (Saccharomycetales, Debaryomycetacae) from China, MycoKeys 89, pp. 121-137 : 121

Kodamaea yamadae C.Y. Chai & F.L. Hui sp. nov.

Fig. 4

Holotype.

China, Henan Province, Nanyang City, the Baotianman Nature Reserve, in rotting wood under a mixed forest, August 2016, K.F. Liu & Z.W. Xi (holotype NYNU 168114T, ex-holotype CBS 14703).

Etymology.

The specific epithet Kodamaea yamadae is used in honour of Y. Yamada for his proposal of the genus Kodamaea .

Description.

In YM broth, after three days at 25 °C, cells are ellipsoidal to elongate (2-3 × 4.5-10 μm) and occur singly or in pairs (Fig. 4a ). Sediment is formed after a month, but a pellicle is not observed. On YM agar, after 3 days at 25 °C, colonies are white, convex, sometimes fringed, glabrous or membranous, smooth or rugose and butyrous to tough due to filamentous growth. On Dalmau plate culture on CM agar, a rudimentary pseudomycelium is formed (Fig. 4b ). Asci or signs of conjugation are not seen on sporulation media. Glucose, maltose, sucrose, trehalose and cellobiose are fermented but not galactose, melibiose, lactose, melezitose, raffinose, inulin or xylose. Glucose, galactose, glucosamine, d-ribose, d-xylose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, inulin, glycerol, erythritol, ribitol, d-glucitol, d-mannitol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, succinate, citrate and ethanol are assimilated as sole carbon sources. l-sorbose, d-arabinose, l-arabinose, l-rhamnose, arbutin, melibiose, lactose, raffinose, melezitose, xylitol, galactitol, myo -inositol, 5-keto-d-gluconate, d-gluconate, d-glucuronate, dl-lactate and methanol are not assimilated. Ethylamine, l-lysine, creatine, glucosamine and d-tryptophan are assimilated as sole nitrogen sources. Nitrate, nitrite, cadaverine, creatinine and imidazole are not assimilated. Growth is observed at 30 °C but not at 35 °C. Growth in the presence of 0.1% cycloheximide is positive, but growth in the presence of 10% NaCl plus 5% glucose and 1% acetic acid is negative. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolates examined.

China, Henan Province, Nanyang City, the Baotianman Nature Reserve, in rotting wood under an oak forest, August 2016, K.F. Liu & Z.W. Xi (NYNU 16858).

Notes.

Two strains NYNU 16858 and NYNU 168114, representing K. yamadae clustered in a well-supported clade that is closely related to K. jinghongensis ( Gao et al. 2017) and K. fukazawae ( Nakase et al. 1999). The nucleotide differences between K. yamadae and K. jinghongensis were 2.8% substitutions in the LSU rDNA sequences and 3.9% substitutions in the ITS region. Similarly, K. yamadae and K. fukazawae showed differences of 2.6% substitutions in the LSU rDNA sequences and 4.7% substitutions in the ITS region. Physiologically, the novel species differed from K. jinghongensis by its ability to ferment cellobiose and its inability to assimilate arbutin. Unlike K. fukazawae , the novel species was able to assimilate d-galactose, l-sorbose, inulin, d-arabinose, l-arabinose, l-rhamnose, and methanol, and was not able to grow in the presence of 0.1% cycloheximide. In all cases, identification by sequencing was the best approach.

Figures

Figure 4. Morphology of Kodamaea yamadae (NYNU 168114, holotype) a budding cells in YM broth after 3 d b pseudohyphae on CM agar after 14 d. Scale bars: 10 μm.