Ortleppascaris sp.
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publication ID |
https://doi.org/ 10.1016/j.ijppaw.2014.03.003 |
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persistent identifier |
https://treatment.plazi.org/id/975887E5-FFB6-FFFA-6169-6483FDA2F971 |
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treatment provided by |
Felipe |
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scientific name |
Ortleppascaris sp. |
| status |
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2.2. Preparation of a somatic extract of Ortleppascaris sp.
Somatic protein extracts were obtained by macerating approximately 200 larvae in extraction solution (7 M urea, 2 M thiourea, 2% CHAPS) whilst being cooled with liquid nitrogen and then centrifuging the extracts at 13,000 ×g for 15 min at 4 ° C. The supernatant was directly used for protein analysis .
No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.