Leptolyngbya boryana ( Gomont 1899: 36 ) Anagnostidis et Komárek (1988: 391)

Cunha De Oliveira, Elane D., Da Cunha, Alan C., Da Silva, Natalina B., Castelo-Branco, Raquel, Morais, João, Schneider, Maria Paula C., Faustino, Silvia M. M., Ramos, Vitor & Vasconcelos, Vitor, 2019, Morphological and molecular characterization of cyanobacterial isolates from the mouth of the Amazon River, Phytotaxa 387 (4), pp. 269-288 : 278

Leptolyngbya boryana ( Gomont 1899: 36) Anagnostidis et Komárek (1988: 391) ( Fig 2C )

Strains:— LEGE 15486 (MF629803)

Description:— The filaments are entangled, forming a dark-green biofilm attached to the culture flask. In aged cultures, a characteristic ferruginous color can be observed for this strain. Trichomes are flexuous, immotile and non-attenuated. They are constricted at the cross-walls and have necridic cells and thin hyaline sheaths ( Fig. 2C ). The cells are subquadratic, i.e., the cells are longer than wide (1.6–2.9 μm long and 1.9–2.6 μm wide), and pale blue-green in color.

Phylogeny and polyphasic identification:— The strain LEGE 15486 shares 100% identity with the sequences from four strains of Leptolyngbya Anagnostidis & Komárek (1988: 390) , namely Leptolyngbya sp. NIES-504; Leptolyngbya sp. KSU-AQIQ-12 and KSU-AQIQ-6; and Leptolyngbya sp. CENA104 ( Table S1). The 16S rRNA gene sequence of LEGE 15486 shares 99.4% similarity with L. boryana PCC 6306 ( Table 4), a reference strain according to Bergey’s Manual ( Castenholz et al. 2015). These strains along with other Leptolyngbya boryana and Leptolyngbya sp. strains clustered in a monophyletic clade with very good node support (ML/BI/MP = 100/1.00/100) ( Fig. 3 ). Based on the polyphasic evaluation of LEGE 15486, this strain was identified as L. boryana ( Komárek & Anagnostidis, 2005) .

Figures

FIGURE 2 Microphotographs of the Amazonian cyanobacterial strains: A) Alkalinema aff. pantanalense LEGE 15484, B) Limnothrix cf. planctonica LEGE 15485, C) Leptolyngbya boryana LEGE 15486, D) Cephalothrix lacustris LEGE 15487, E) Phormidium sp. LEGE 15488, F) Pseudanabaena cf. galeata LEGE 15489, G) P. cf. galeata LEGE 15490, H) P. cf. galeata LEGE 15491, I) Cephalothrix lacustris LEGE 15492, J) C. lacustris LEGE 15493; K) unidentified Nostocales LEGE 15494, L) unidentified Nostocales LEGE 15495, M) unidentified Nostocales LEGE 15496, and N) Limnothrix cf. planctonica LEGE 15497.

FIGURE 3. ML phylogenetic tree based on 16S rRNA gene sequences showing the placement of the Amazonian cyanobacterial strains (in bold). Bootstrap values>70% or Posterior Probabilities>0.90 are indicated below the clade nodes (ML/BI/MP). Each strain sequence is identified by its accession number. The tree was rooted with the outgroup Chloroflexus aurantiacus J-10-fl (NR_074263), which was pruned from the tree for clarity. (T)refers to type strains according to CyanoType (Ramos et al. 2017).