Teunia qingyuanensis Y. Z. Qiao & F. L. Hui, 2024

Guo, Qi-Chao, Liu, Shan, Qiao, Ya-Zhuo & Hui, Feng-Li, 2024, Three new species of Teunia (Cryptococcaceae, Tremellales) identified through phenotypic and phylogenetic analyses, MycoKeys 105, pp. 139-153 : 139-153

Teunia qingyuanensis Y. Z. Qiao & F. L. Hui sp. nov.

Fig. 3 B

Etymology.

Referring to the locality, Qingyuan Mountain, where the type strain was collected.

Typus.

China. Fujian Prov. Quanzhou City, Qingyuan Mountain ; in rotting wood; Mar 2022; W. T. Hu & S. B. Chu; NYNU 22475 (holotype GDMCC 2.294 T, culture ex-type PYCC 9929 ; GenBank Nos: OP 269841 , OP 269842 , PP 236728 ) .

Description.

On YM agar after seven days at 20 ° C, the streak culture was cream, mucoid and smooth, with an entire margin. After seven days in YM broth at 20 ° C, single cells were ovoid to ellipsoidal, 3–7 × 4–7.5 μm, budding polar. After one month at 20 ° C, sediment was present. In Dalmau plate culture on CMA, no hyphae or pseudohyphae were formed. Sexual structures were not observed on PDA, CMA or V 8 agar. Glucose fermentation was absent. Glucose, inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, salicin, L-sorbose (weak), L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol, glycerol, ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, D-gluconate, 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone were assimilated as carbon sources; methanol, erythritol, and D-glucosamine were not assimilated. Ethylamine and L-lysine were assimilated as nitrogen sources; nitrate, nitrite, and cadaverine were not assimilated. Maximum growth temperature was 30 ° C. Growth in vitamin-free medium was positive. Growth on 50 % (w / w) glucose-yeast extract agar was negative. Starch-like substances were not produced. Urease activity and Diazonium Blue B reaction were positive.

Note.

In the molecular analysis (Fig. 2 ), T. qingyuanensis sp. nov. formed a distinct clade together with T. cuniculi . They can be differentiated by the ability of T. qingyuanensis sp. nov. to grow in vitamin-free medium and to assimilate raffinose, melibiose, and DL-Lactate.

Figures

Figure 2. Maximum likelihood phylogenetic tree of Teunia generated from the combined ITS, LSU, and RPB 1 sequence data. The tree is rooted with Cryptococcus amylolentus CBS 6039 T and Cryptococcus neoformans CBS 8710 T. Bootstrap values (MLBS ≥ 50 % and BPP ≥ 0.95) are displayed near branches. Type strain sequences are marked with (T).

Figure 3. Vegetative cells of A T. acericola sp. nov. NYNU 2111141 T B T. qingyuanensis sp. nov. NYNU 22475 T and C T. mussaendrae sp. nov. NYNU 23232 T, following 7 days of growth in YM broth at 20 ° C. Scale bars: 10 μm.