Endomelanconiopsis freycinetiae Tibpromma & K.D. Hyde

Tibpromma, Saowaluck, Hyde, Kevin D., Bhat, Jayarama D., Mortimer, Peter E., Xu, Jianchu, Promputtha, Itthayakorn, Doilom, Mingkwan, Yang, Jun-Bo, Tang, Alvin M. C. & Karunarathna, Samantha C., 2018, Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand, MycoKeys 33, pp. 25-67 : 33-34

Endomelanconiopsis freycinetiae Tibpromma & K.D. Hyde sp. nov. Figure 6

Etymology.

name referring to the host genus on which the fungus was found ( Freycinetia ).

Holotype.

MFLU 18-0002

Culture characteristics.

Colonies on PDA (Figure 2, FE41), superficial, initially white-grey with flat mycelium on media with dark centre, later becoming dark olivaceous with circular rings and flossy at the margin; reverse dark olivaceous. Generative hyphae simple-septate, branched, sub-hyaline to brown, cylindrical, guttulate, thick-walled. Not sporulating in culture (Figure 6).

Material examined.

THAILAND, Ranong, Muang, on healthy leaves of Freycinetia sp. ( Pandanaceae ), 3 December 2016, S. Tibpromma FE41 (MFLU 18-0002, holotype); HKAS100853, paratype, ex-type living cultures, MFLUCC 17-0547 = KUMCC 17-0292.

GenBank numbers.

ITS=MG646955, LSU=MG646948, TEF1=MG646983, β-tubulin =MG646924.

Notes.

Endomelanconiopsis freycinetiae is closely related to the endophytic fungus En. endophytica . Therefore, the culture characteristics of these two taxa were compared and it was found that, in En. endophytica , at first the hyphae are colourless, immersed, later becoming olivaceous in the centre with irregular concentric rings; aerial mycelia are dark olivaceous or grey when dense; shiny black when the aerial mycelia are loose ( Rojas et al. 2008) whereas aerial mycelia of En. freycinetiae has dark olivaceous, circular rings and flossy surface (Figure 2, FE41). Nucleotide base pairs of ITS and TEF1 were also compared and it was found that there are differences (ITS 3 bp, TEF1 8 bp).