Ceriporia albomellea Yuan Yuan, Jia J. Chen & X.H. Ji, 2017

Yuan, Yuan, Ji, Xiao-Hong, Wu, Fang & Chen, Jia-Jia, 2017, Ceriporia albomellea (Phanerochaetaceae, Basidiomycota), a new species from tropical China based on morphological and molecular evidences, Phytotaxa 298 (1), pp. 20-28 : 23-25

Ceriporia albomellea Yuan Yuan, Jia J. Chen & X.H. Ji , sp. nov. ( Figs. 1–2 )

MycoBank: MB 817518

Diagnosis. —Differs from other Ceriporia species by thin, resupinate basidiome with a white subiculum, cottony margin, white to cinnamon-buff pores, clavate cystidia and oblong-ellipsoid basidiospores measured as 3.1–3.8 × 1.7–2 μm.

Type.— CHINA. Hainan Prov.: Qiongzhong County, Limushan Nat. Res., on rotten angiosperm wood, 30 May 2015, Dai 15205 (holotype in BJFC!).

Etymology.— Albomellea (Lat.) refers to the white to cinnamon-buff pore surface.

Fruiting body. —Basidiomes annual, resupinate, soft corky when fresh, brittle when dry, up to 15 cm long, 6 cm wide, and 0.4 mm thick at center. Pore surface white when fresh, cinnamon-buff when dry; pores round to angular, 5–7 per mm; dissepiment thin, slightly lacerate. Margin thin, white to cream, cottony, up to 5 mm wide. Subiculum white, cottony when dry, up to 0.3 mm thick. Tubes concolorous with pore surface, brittle when dry, up to 0.1 mm long.

Hyphal structure. —Hyphal system monomitic; generative hyphae bearing simple septa, IKI–, CB+; tissues unchanged in KOH.

Subiculum. —Generative hyphae hyaline, slightly thick-walled to thick-walled with a wide lumen, frequently bearing simple septa, frequently branched, interwoven, 3–6 μm in diam.

Tubes. —Generative hyphae hyaline, thin- to slightly thick-walled with a wide lumen, frequently bearing simple septa and branched, interwoven, 2.5–3.5 μm in diam; cystidia present, clavate, hyaline, thin-walled, 20–40 × 6–9 μm; basidia more or less barrel-shaped, with four sterigmata and a simple basal septum, 8–11 × 3.5–5 μm; basidioles in shape similar to basidia, but slightly smaller; some rhomboid crystals present among trama.

Basidiospores. —Basidiospores oblong-ellipsoid, hyaline, thin-walled, smooth, occasionally bearing a small guttule, IKI–, CB–, (3–)3.1–3.8(–3.9) × (1.6–)1.7–2 (–2.1) μm, L = 3.43 μm, W =1.86 μm, Q = 1.82–1.86 (n=60/2).

Additional specimens examined.— CHINA. Hainan Prov.: Qiongzhong County, Limushan Nat. Res., on rotten angiosperm wood, 30 May 2015, Dai 15223 (paratype in BJFC!).

Type of rot.— White rot.

Molecular phylogeny

The combined ITS and nLSU dataset included 62 sequences of ITS and 53 sequences of nLSU regions from 65 fungal samples representing 34 species. The dataset had an aligned length of 1961 characters in the dataset, of which 1214 characters are constant, 171 are variable and parsimony-uninformative, and 576 are parsimony-informative. Maximum parsimony analysis yielded 5 equally parsimonious trees (TL = 3121, CI = 0. 397, RI = 0.689, RC = 0.273, HI = 0.603), and one of the maximum parsimonious trees was shown in Fig. 3 . The best model for the combined ITS and nLSU sequences dataset estimated and applied in the BI was GTR+I+G. BI resulted in a similar topology with an average standard deviation of split frequencies = 0.008540 to MP analysis, and thus only the MP tree was provided. Both bootstrap values (≥50%) and BPPs (≥0.95) are shown at the nodes ( Fig. 3 ).

Judging from our molecular analysis, Ceriporia is a heterogeneous assemblage, and some species of other genera within Phanerochaetaceae were clustered together with Ceriporia ( Fig. 3 ). Two sampled specimens of the new species, C. albomellea , formed a well-supported lineage (100% MP and 1.0 BPPs), indicating that they are phylogenetically distinct from other species in the Phanerochaetaceae ( Fig. 3 ). Furthermore, the sequences of C. albomellea clustered together with C. variegata B.S. Jia & Y.C. Dai (90% MP and 0.58 BPPs), then formed a distinct and well supported clade with C. crassitunicata Y.C. Dai & Sheng H. Wu (100% MP and 1.0 BPPs).

Figures

FIGURE 1. A fresh basidiome of Ceriporia albomellea (Dai 15223). Scale bar = 3 cm.

FIGURE 2. Microscopic structures of Ceriporia albomellea (Dai 15205). a Basidiospores.b Basidia and basidioles. c Cystidia. d Rhombic crystals. e Hyphae from trama. f Hyphae from subiculum.

FIGURE 3. Strict consensus tree illustrating the phylogeny of Ceriporia albomellea and its related species generated by maximum parsimony based on ITS+nLSU sequences. Branches are labeled with parsimony bootstrap proportions (before slanting line) high than 50% and bayesian posterior probabilities (after slanting line) more than 0.95.