Diaporthe rosae Samarakoon & K.D. Hyde, Fungal Diversity

Huanraluek, Naruemon, Phunyaboon, Wilawan & Jayawardena, Ruvishika S., 2022, A new host record of Diaporthe rosae on Rambutan (Nephelium lappaceum L.), Phytotaxa 567 (2), pp. 162-172 : 168

Diaporthe rosae Samarakoon & K.D. Hyde, Fungal Diversity 89: 185. 2018. Fig. 3

Index Fungorum number: IF 554072; Facesoffungi number: FoF 03922

Associated with branches of Nephelium lappaceum (L.). Sexual morph: Not observed. Asexual morph: Conidiomata pycnidial in culture on PDA at 25 °C after 14 days, globose to oval, solitary or aggregated in clusters, semi-immersed, black. Conidiomatal well paraenchymatous, consisting of 4–5 layers of pale brown, thick-walled cells of textura angularis. Conidiophores cylindrical, hyaline, smooth, unicellular, 7–8 × 3–4 µm. Conidiogenous cells phialidc, clavate, tapering towards the apex, 14–17 × 2–5 µm. Alpha-conidia unicellular, ovoid, (5–)6–7(–9) × (1–)2–3(–4) µm, (= 6.7 × 2.3 µm, n = 29), hyaline, bi- guttulate, aseptate, smooth-walled. Beta-conidia hyaline, aseptate, filiform, bent, eguttulate, wider at the center and thinner towards the ends, similar to a hook, (8–)11–14(–16) × (1–)2(3) µm, (= 13.1 × 1.2 µm, n = 11).

Culture characteristics:— The aerial fluffy mycelium appeared white with emerging dark pigmentation spots along with the production of enormous black stromata on PDA. The colony color on the reverse side was yellow, reproduced asexually and pycnidia released a spore bulk containing Alpha-conidia and Beta-conidia.

Material examined:— THAILAND. Chiang Rai Province: on discolored branches of Nephelium lappaceum , 7 September 2020, Kushan Mallikarathna RR 9 ( MFLU 22-0049 , living culture MFLUCC 22–0011 ) .

Notes:— Diaporthe rosae MFLUCC 22–0011 was observed growing on branches of rambutan, ability to produce pycnidial in the cultural presence of an asexual morph have both conidia, α ( Alpha ) and β ( Beta ), the character of morphology it the similarity in type species ( D. rosae MFLUCC 17–2658). Combined multi-gene analysis shows that D. rosae (MFLUCC 22–0011) clusters with the ex-type strain of D. rosae (MFLUCC 17–2658) with 97/--/0.98 (ML/ MP/BYPP). Based on morphology and phylogenetic data MFLUCC 22–0011 is identified as D. rosae and is reported as a new host record from Thailand. A comparison of our new collection with Diaporthe rosae recorded from Thailand from other hosts are given below ( Table 2 ).

Figures

FIGURE 3. Diaporthe rosae (MFLUCC 22–0011) a Herbarium material.b–e Sporulation on PDA.Mycelium on PDA.g–h Conidiophores with Alpha-conidia. j–k Alpha-conidia. l–m Alpha-conidia and Beta–conidia. Scale bars: f, g, h, i = 10 µm, j, k, l, m = 5 µm.