Pista colini, Labrune, Celine, Lavesque, Nicolas, Bonifacio, Paulo & Hutchings, Pat, 2019

Labrune, Celine, Lavesque, Nicolas, Bonifacio, Paulo & Hutchings, Pat, 2019, A new species of Pista Malmgren, 1866 (Polychaeta, Terebellidae) from the north-western Mediterranean Sea, ZooKeys 838, pp. 71-84 : 74-79

Pista colini sp. n. Figs 2, 3, 4

Material examined.

Type material. Banyuls-sur-Mer harbour, Gulf of Lion, Mediterranean Sea, France (42°28.867'N, 3°08.154'E, 3 m depth), subtidal in gravely sands, all collected 16 July 2012 except MNHN-IA-TYPE 1853 collected 12 July 2017. Holotype: MNHN-IA-TYPE 1850, complete, 70 segments, total length 17.6 mm, thoracic length 4.8 mm, anterior width 0.6 mm, Paratypes: AM W.50625, 1 specimen, posteriorly incomplete, total length 11 mm, thoracic length 7 mm, anterior width 1.0 mm; AM W.50626, 3 specimens plus 1 posterior fragment 5 mm with pygidium, 1 complete, total length 11 mm, thoracic length 5 mm, anterior width 0.5 mm, 1 complete, total length 12 mm, thoracic length 5 mm, anterior width 0.5 mm, 1 posteriorly incomplete, length 16 mm, thoracic length 8 mm, anterior width 0.8 mm, 2 specimens mounted for SEM. MNHN-IA-TYPE 1851, 1 specimen, posteriorly incomplete, total length 14.3 mm, thoracic length 3.7 mm, anterior width 0.7 mm; MNHN-IA-TYPE 1852, complete specimen, total length 9.70 mm, thoracic length 4.6 mm, anterior width 0.9 mm; MNHN-IA-TYPE 1853, complete collected 12 July 2017, thoracic length 4.4 mm, anterior width 1.1 mm, posterior part cut for molecular analysis; MNHN-IA-TYPE 1854, complete length 18.2 mm, anterior width 0.7 mm, mounted for SEM; MNHN-IA-TYPE 1855, complete, 1 specimen, total length 9.0 mm, thoracic length 3.1 mm, anterior width 0.7 mm.

Additional material. Banyuls-sur-Mer harbour, Gulf of Lion, Mediterranean Sea, France (42°28.867'N, 3°08.154'E, 3 m depth), subtidal in gravely sands, all collected 16 July 2012. BAN.Pista.08, 1 specimen, complete, total length 10.0 mm, thoracic length 3.7 mm, anterior width 0.8 mm; BAN.Pista.09, 1 specimen gravid, posteriorly incomplete, thoracic length 5.1 mm, anterior width 0.7 mm; BAN.Pista.10, complete, 1 specimen, total length 22.7 mm, thoracic length 7.8 mm, anterior width 0.9 mm; BAN.Pista.12, complete, 1 specimen, total length 12.4 mm, thoracic length 4.6 mm, anterior width 0.6 mm.

Comparative material. Pista bansei Saphronova, 1988 Holotype reg. # 47667, 47°41'N, 139°34.1'E, Sea of Japan, Tartary Strait, off Nelma, 105 m; 4 paratypes reg. # 47668 according to Saphronova 1988 (but reg. # 32423 according to label in the museum vial) from same station, deposited in Zoological Museum of Russian Academy of Sciences in St Petersburg.

Additional material from R/V “Vityaz” stations 59, 119, 1587a, 3350, 3569, 1086. (For locality details see Saphronova (1988: table 1, no museum registration numbers allocated) deposited in the Zoological Museum of Moscow State University.

Description

(based on holotype). Holotype is a complete specimen, 17.5 mm in length, 0.6 mm in width at segment X and with 70 segments (Fig. 2A, B).

Transverse prostomium attached to dorsal surface of upper lip. Buccal tentacles all of similar width inserted ventrally on prostomium, shorter than smallest branchia; long tentacles situated centrally in dorsal region, longer than largest branchia (Fig. 2C). Peristomium consisting of large rounded upper lip, forming a swollen cushion with one small fold on each side. Lower lip short, irregularly swollen (Fig. 3C). Segment I reduced, V-shaped, situated medio-ventrally (Fig. 3C), without lateral lobes. Segment II with well-developed lateral lobes, with anterior margins rounded merging with ventral pad to form a continuous minutely crenulated ventral collar. One pair of unequal-sized plumose branchiae inserted one just next to the other on segment II; all filaments strongly ciliated (Figs 2D, 3D), arranged in spiral around central axis with dichotomous filaments. Both stalks markedly wrinkled (Fig. 3A, B). Segment III with lateral lobes half width of segment, asymmetrical and slightly displaced dorsally, connected across ventrum (Fig. 3 A–C). Segment IV lacking lateral lobes (Fig. 3A, B).

Notochaetae, broad-winged capillaries, with fine tips (Fig. 4A). Neuropodia from segment V (chaetiger 2), initially arranged in single rows, from segments XI to XX arranged in completely intercalated double rows face-to-face and then reverting to single rows on abdomen. Neurochaetae as long-handled avicular uncini on segments V and VI (Fig. 4B) then short-handled. Neuropodia with ca. 14 uncini (arranged in single row), thoracic uncini with dental formula MF: 3 –4:5– 6:α (Fig. 4 D–E). Abdominal neuropodia becoming more erect posteriorly with ca. 12 uncini each, elongate extending from torus, dental formula MF: 6 –7:6– 7: α: α (Fig. 4C, F). Nephridial papillae on segments VI and VII (chaetigers 3 and 4), inserted posteriorly/laterally to notopodia, small spherical.

Pygidium with slightly crenulated margins (hardly visible even under stereomicroscope but clearly visible under SEM).

Methyl green staining pattern.

Branchiae, lips and base of tentacles not stained. Extremity of tentacles staining and retained as blue/brown even after being washed in ethanol for some days (Fig. 2B, C). Thorax until segment XX, strongly staining ventrally, moderately laterally and poorly dorsally. Ventral stain on all shields, anterior half of each shield staining deeply, posterior part not staining (Fig. 2B, C). Anterior abdomen not coloured and posterior abdomen staining ventrally and dorsally with anterior half of each segment staining deeply, posterior part not staining; increasing colouration towards pygidium (Fig. 2B).

Morphological variation.

Complete individuals ranging from 9.0 to 22.7 mm in length, 0.5 to 1.1 mm in width at segment X and between 59 to 72 segments. Thoracic lengths vary between 3.1 and 7.8 mm. One gravid specimen was found (BAN.Pista.09). It was incomplete, but thoracic length was 5.1 mm and anterior width 0.7 mm. These measurements correspond to a small size species. Live specimens pinkish with translucent buccal tentacles; ventral shields divided in two parts, anterior part pinkish, posterior part blood red (Fig. 2A). Preserved specimens pinkish with ventral shields divided transversally in two parts. Crenulation of ventral collar of segment II is difficult to see under the binocular and not always visible under SEM. It probably depends on the contraction of the animal. All specimens, regardless of size, with a single pair of branchiae, one up to twice as long as the other (Fig. 2D). Eleven of the twenty observed specimens had the long branchiae on the right side. Number of anterior thoracic uncinigers with long-handled uncini is variable (from 2 to 9). This difference seems to not be dependent upon size. Nephridial papillae not always visible.

Etymology.

The name of species is dedicated to the nephew of the first author Colin Labrune who is already a little budding naturalist.

Type locality.

Only known from Banyuls-sur-Mer harbour, France (Mediterranean Sea).

Ecological notes.

Pista colini sp. n. was sampled at 3 m depth on gravelly sand recently deposited manually in Banyuls-sur-Mer harbour. It was found in very high densities (446 ind. m-2 in April 2012 and 1176 ind. m-2 in July 2012) a few weeks after the sediments had been deposited. We sampled again in November 2012 but there was no more gravel and Pista colini sp. n. was absent. The species is not found in the harbour if no gravel deposits are present. In the undisturbed part of the harbour, median granulometry was ca. 50 µm while the median granulometry of the gravelly sand in which this species is found was ca. 800 µm. In July 2017, we sampled a week after another fresh load of sediment with gravel had been deposited and we found high densities of Pista colini sp. n. living in tubes made from heterogeneous sediment agglomerated with mucus.

Genetic data.

The COI gene was successfully sequenced and published at NCBI GenBank for paratype MNHN-IA-TYPE 1853 with accession number MK584933.

Remarks and discussion.

The presence of a single pair of branchiae is a stable character in Pista colini sp. n. More than 100 specimens were observed, of different sizes and all of them had a single pair of branchiae, some were also observed alive. Our observations support Hutchings et al. (2017) but are not in agreement with Saphronova and Jirkov (2001), who hypothesised that this character is size-related. A detailed morphological and molecular study needs to be performed in order to investigate this hypothesis across a range of species with varying number of pairs of branchiae.

Although Gil (2011) mentioned Pista cristata as having only one pair of branchiae, based on Saphronova (1988), we considered that this species has two pairs of branchiae. The absence of consensus on this species does not have any consequence for this new species which always had some long-handled thoracic uncini, whereas Gil (2011) records P. cristata as lacking such long-handled even on large specimens. Among the seven species with a single pair of branchiae, there is no possible confusion of P. colini sp. n. with Pista mirabilis and with P. spinifera as both lack plumose branchiae. Pista colini sp. n. is close to P. adriatica sharing the following characters: one pair of unequal sized plumose branchiae on segment II and presence of lateral lobes on segments II and III, lacking on segment IV. However, segment II of P. adriatica presents narrow lateral lobes while in P. colini sp. n. these lateral lobes are well developed. Lateral lobes of segment III are rectangular in P. adriatica rather than being asymmetrical and slightly displaced dorsally as in P. colini sp. n. Furthermore P. colini sp. n. can be differentiated by the absence of glandular ridges on segments II and III, which are present in P. adriatica . According to Mikac and Hutchings (2017), P. godfroyi and P. dibranchis which also have a single pair of branchiae, should be transferred to Pistella Hartmann-Schröder 1996 because they lack long-handled uncini. Therefore, they cannot be confused with P. colini sp. n. The lack of long-handled uncini is also the case for Pista lornensis. Furthermore, when first describing Pista lornensis , Pearson (1969) reported two obvious ligaments, one attached below the rostrum and the largest to the posterior basal corner of the uncini. These filaments are not present in P. colini sp. n.

According to Gil (2011), Pista bansei is the only Pista species in Europe to present one pair of "pompom like" branchiae and anterior long-handled uncini. The original description by Saphronova (1988) is based on an incomplete holotype with 16 segments, 3.2 mm wide collected at 105 m in Strait of Tartar, the Sea of Japan, north-western Pacific Ocean, and four damaged paratypes from the same locality. She also designated another eight paratypes (R/V “Vityaz” St 1576, 60°03'N, 168°46'E, 230 m, Olutorsky Bay, off Kamchatka Peninsula, Bering Sea, north-western Pacific Ocean) and 1 paratype (R/V “Sevastopol” St 1086, 495 m, 62°56'N, 9°19'W, between Iceland and Faroe Islands, North Atlantic Ocean), the material is deposited in Zoological Museum of Moscow State University. She also lists additional specimens not designated as type material from localities such as Davis Strait, Norwegian, Kara Sea (off Novaya Zemlya), White Sea in the North Atlantic, and Arctic Oceans, as well as Sea of Ja pan, Sea of Okhotsk, and Bering Sea in the north-western Pacific Ocean in depths of 120-606 m. Such a wide distribution is highly unlikely and we suggest that P. bansei sensu stricto is restricted to the north-western Pacific Ocean, while the rest of the material, including the one paratype from the North Atlantic Ocean represents another species, most likely part of the same species complex. Although, much of the material in Zoological Museum of Moscow State University is in poor condition, it most certainly belongs to multiple species. Therefore, Saphronova’s (1988) hypothesis that only adults have anterior thoracic uncini with well-developed handles, while such handles are absent in juveniles, cannot be accepted. Furthermore, her diagrammatic illustrations indicate neither the sizes of individuals nor where the specimens were collected.

All the specimens of P. colini sp. n. examined here, even the smallest (59 chaetigers, thoracic width at segment X: 0.5 mm), which are comparable in size with the individuals that Saphronova (1988) identified as juveniles (width between 0.4 and 1.15mm), had well-developed long-handled uncini, at least on chaetigers 1 and 2. Furthermore, in the original description, Saphronova (1988) described P. bansei with (1) an upper lip high and narrow while upper lip of P. colini sp. n. is large and rounded (2) large lateral lobes, positioned vertically and connected mid ventrally by a wide fold, although the connection between the two lateral lobes in P. colini sp. n. does not form a fold and does not look like the illustration in Saphronova (1988, fig. 8 g–i). Moreover, Hilbig (2000) reports P. bansei with (1) moderate numbers of tentacles, usually broken off, although all specimens of P. colini sp. n. had some short and some long tentacles, rarely broken and (2) glandular ridges on segments II and III, which were not observed in P. colini sp. n. Furthermore, based on the holotype 1/47667, paratypes, and several additional specimens, Jirkov and Leontovich (2017) reported the presence of small lateral lobes on segment I in P. bansei which are not observed in P. colini sp. n. Therefore, P. colini sp. n., while similar to P. bansei in a number of characters, differs by the presence of long-handled uncini, even in the smallest specimens, and the fact that no glandular ridge was observed on segments II and III. Furthermore, the type locality of P. bansei is from the northern Pacific in cold deeper water (105 m).

Based on examination of the type material of Saphronova (1988) in Moscow and St Petersburg museums by Hutchings in 2018 (see Comparative material), Pista bansei is a North Pacific species currently only known with certainty from Tartar Strait and therefore, its range cannot overlap with that of any Mediterranean species. For these reasons, we describe P. colini as a new species from the Mediterranean Sea. Finally, this paper reinforces the need for a complete revision of the group of terebellids with long-handled uncini using both molecular and morphological data, especially those species with only a single pair of branchiae.