Cladosporium puris M.L.R. Freitas & O.L. Pereira, 2021

Freitas, Mayara L. R., Gomes, André A. M., Rosado, André W. C. & Pereira, Olinto L., 2021, Cladosporium species from submerged decayed leaves in Brazil, including a new species and new records, Phytotaxa 482 (3), pp. 223-239 : 232

Cladosporium puris M.L.R. Freitas & O.L. Pereira sp. nov. ( Figure 2 )

MycoBank: MB826908

Systematic position: Fungi, Ascomycota, Pezizomycotina, Dothideomycetes, Dothideomycetidae, Capnodiales , Cladosporiaceae .

Etymology:—The name reflects a native indigenous tribe that lived in the region where the fungus was collected.

Colonies on PDA attaining 36 mm diam after 14 days, at 25 ºC, under near-ultraviolet light, dark green to olive green, velvety, furrowed; reverse olivaceous grey. On MEA, colonies attaining 57 mm diam after 14 days, at 25 ºC, under near-ultraviolet light, dark green to olive green, pale olivaceous grey central mycelium; velvety; reverse olivaceous grey. Mycelium sparsely formed, 1.5–2 µm wide, pluriseptate, medium to dark brown, sometimes subhyaline, smooth or asperulate. Conidiophores solitary, cylindrical, 44−225 × 2−3 µm, arising terminal and laterally from hyphae, erect or somewhat flexuous, dark brown, septum conspicuous and thickened walls. Conidiogenous cells integrated, mainly terminal, cylindrical, sometimes geniculate-sinuous, sympodial proliferation, 7.5−42.5 × 2−3.5 µm, thickened and darkened conidiogenous loci (1–3). Ramoconidia cylindrical, 8−17.5 × 2.5−4 µm, 0−1 septum, pale brown, truncate base, somewhat thickened. Secondary ramoconidia oblong, oblong-ellipsoid, 5−12.5 × 2−3.5 µm, 2−3 distal hila, narrowed base, pale brown. Intercalary conidia numerous, catenulate, acrogenous, branching in all directions, ellipsoidal, aseptate, medium to dark brown, 3.5−6 × 2−3.5 µm, protuberant hila. Small terminal conidia subglobose to ellipsoidal, 2.5−4.5 × 2−3 µm, aseptate, medium to dark brown, protuberant hila.

Type:— Brazil. Minas Gerais: Canaã, 626–642 m, 20°44’24” S, 42°37’53” W, on submerged litter in streams, September 7, 2017 (holotype VIC 44468 , ex-type culture COAD 2487). GenBank: MK253337 (ITS), MK249980 (ACT), MK293777 (TEF1-α)

Additional specimens examined: — BRAZIL. Minas Gerais: Canaã, 626–642 m, 20°44’24” S, 42°37’53” W, on submerged litter in streams, 7 September 2017 (living culture COAD 2494 ) .

Figures

FIGURE 2. Cladosporium puris (VIC 44468, holotype). A. Tombo da Cachoeira, Canaã-MG. B. Leaf litter submerged in the watercourse. C –D. Colonies on Malt Extract Agar and Potato Dextrose Agar, after 14 days, at 25 ºC, under near-ultraviolet light, respectively. E. Overview of reproductive structures of the fungus. F. Ramoconidia and conidia formed in acropetal chains. G. Secondary ramoconidia. H. Conidia. Scale bars = 10µm.