Parapheretima Cognetti, 1912

Aspe, Nonillon M. & James, Samuel W., 2016, NeW species OF Pheretima, Amynthas, Polypheretima, and Pithemera (Clitellata: MegascOlecidae) FrOm MindanaO and assOciated islands, Philippines, Zoological Studies 55 (8), pp. 1-33 : 18-20

Subgenus Parapheretima Cognetti, 1912

Type species: Pheretima aberrans Cognetti 1911 .

Diagnosis: Pheretima species with a secretory diverticulum discharging into each copulatory bursa.

Pheretima (Parapheretima) pandanensis n. sp. ( Fig. 3E , Table 3 ) urn:lsid:zoobank.org:act:6B9AB7F7-FEF2-43F3-89C3-12503816176D

Material examined: Holotype: adult ( NMA 4593 ), Brgy Pandan , Mambajao City, Camiguin Province, Mt. Timpoong (9°10'45"N, 124°43'15"E), 1350 m asl, Camiguin Island, Philippines, coll. N. Aspe, J. Adeva, 11-15 Oct. 2004 . Paratypes: five adults ( NMA 4609); four adults ( ZRC. ANN 0062), same collection data as for holotype.

Etymology: The species is named for Brgy Pandan of Mambajao City, Camiguin Province, where this species was collected.

Diagnosis: Dark reddish brown worm, adult length 85-150 mm; ventrum pale; pair of spermathecal pores at intersegment 7/8; male pores and spermathecal pores 0.23 and 0.23-0.25 circumference apart; 50-55 setae on xx; 4-9 setae between male pores; single secretory diverticulum projecting from lateral surface of each copulatory bursa; long, tapering penis.

Description: In living animals, dark reddish brown dorsum, lighter ventrum, equators non-pigmented. Length 86-150 mm (n = 10 adults); diameter 4-5 mm at x, 4-5 mm at xx; body circular in cross-section, tail tapering; 85-104 segments. First dorsal pore at 12/13, paired spermathecal pores at 7/8, distance between spermathecal pores 3 mm (0.23 circumference apart ventrally), spermathecal pores inconspicuous in some individuals. Female pore single in xiv, openings of copulatory bursae paired in xviii, distance between openings 3-4 mm (0.23-0.25 circumference apart ventrally), 4-9 setae between openings. Clitellum annular, from xiv to xvi. Setae unevenly distributed page 18 of 33

around equators; 36-44 setae on vii, 50-55 setae on xx, dorsal setal gaps present, no ventral gaps. Genital markings lacking.

Septa 6/7/8 and 10/11-12/13 muscular, 4/5/6 and 8/9/10 lacking. Dense tufts of nephridia on anterior faces of 5/6 and 6/7; nephridia of intestinal segments located mainly on body near septum/body wall junction. Large gizzard in viii-x, esophagus with low vertical lamellae x-xiii, intestinal origin in xiv; caeca simple, originating in xxvii, extending forward to xxv or to xxiv, margins serrated; typhlosole originating in xxvii, simple fold slightly less than lumen diameter, intestinal wall with 31-53 longitudinal blood vessels. Hearts in x to xiii, esophageal; commissural vessels in vi, vii, and ix lateral; those in viii extend to gizzard.

Ovaries and funnels free in xiii. Spermathecae paired, postseptal in viii, with nephridia on ducts. Each spermatheca with round ampulla, very large, short, bulbous, muscular duct; single stalked diverticulum attached to ectal portion of left face of duct close to the ampulla of the right spermatheca, and on right face of duct of left spermatheca; stalks terminating in elongate receptacle, becoming bulbous abruptly midway toward duct. Spermathecae contained two small, ovate spermatophores, lacking in some individuals. Male sexual system holandric, testes and funnels enclosed in paired sacs in x, xi; seminal vesicles xi, xii, each with digitate dorsal lobe; vesicles of xi enclosed in testes sac; vasa deferentia slender, free from body wall on way to ental end of prostatic ducts; prostate glands in two parts, in xvii-xviii, and in xix-xx, each part dense, racemose mass with two or three lobes; long duct from each prostate joins others to form thick, coiling, muscular duct connecting to copulatory bursa. Each copulatory bursa hemispheric in xvii-xix; coelomic surface muscular, with single secretory diverticulum projecting from lateral surface; roof with two pads, floor with thin pads; long, tapering penis arises from padded roof.

Remarks: Pheretima (Parapheretima) pandanensis n. sp. and P. (Paraph.) boaensis n. sp. (see next description) are the first P. (Paraph.) species reported from the Philippines. Pheretima (Paraph.) pandanensis n. sp. differs from other P. (Paraph.) species in the location of the spermathecae, which are paired in intersegment 7/8. Other P. (Paraph.) species are either monothecate (members of the P. (Paraph.) barbara Cognetti 1913 species group), or if bithecate, have the spermathecae located at intersegment 6/7 [P. (Paraph.) bernhardi Gates 1948 or members of

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the P. (Paraph.) beauforti Cognetti 1913 species group] or at intersegments 5/6/7 [P. (Paraph.) saba Sims and Easton 1972]. Pheretima (Paraph.) pandanensis is similar to P. camiguinensis n. sp. in size and coloration, but differs from the latter in having a wider distance between spermathecal pores and between male pores, in having more setae between the male pores, and in having more post-clitellar setae on the segmental equators ( Table 3 ).

Pheretima (Parapheretima) boaensis n. sp. ( Fig. 3F , Table 3 ) urn:lsid:zoobank.org:act:7FC61F12-455E-4A4B-B6BF-A67E884E7C35

Material examined: Holotype: adult ( NMA 4594 ), Brgy Boa , municipality of Cagdianao, Dinagat Province, (10°05'53"N, 125°39'42"E), 250 m asl, Dinagat Island, Philippines, coll. N. Aspe, J. Adeva, 23-26 Apr. 2004 . Paratypes: two adults ( NMA 4610); three adults ( ZRC. ANN 0063), same collection data as for holotype.

Etymology: The species is named after Brgy Boa ion the municipality of Cagdianao, Dinagat Province, where this species was collected.

Diagnosis: Reddish brown worm, adult length 94-133 mm; ventrum pale; single spermathecal pore midventrally at intersegment 5/6; single muscular secretory diverticulum projecting from anterior margin of each copulatory bursa; 6-9 setae between male pores; penis lacking.

Description: Reddish brown dorsum, lighter ventrum, equators pigmented. Length 94-133 mm (n = 6 adults); diameter 3.5 mm at x, 4-4.5 mm at xx; body circular in cross-section, tail tapering; 94- 146 segments. First dorsal pore at 12/13, single midventral spermathecal pore at 5/6. Female pore single in xiv, openings of copulatory bursae paired in xviii, distance between openings 2.3 mm (0.16 circumference apart ventrally), 6-9 setae between openings. Clitellum annular, from xiv to xvi. Setae unevenly distributed around equators in some segments; 41-46 setae on vii, 57-59 setae on xx, dorsal setal gaps present, no ventral gaps. Genital markings lacking.

Septa 4/5/6 thin, 7/8 and 10/11-13/14 muscular, 8/9/10 lacking. Dense tufts of nephridia on anterior faces of 5/6 and 6/7; nephridia of intestinal segments located mainly on body near septum/body wall junction. Large gizzard in viii-x, esophagus with low vertical lamellae x-xiii, intestinal origin in xiv or xv, caeca simple originating in xxvii, extending forward to xxiii.

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One individual with caeca folded upward in xxiii, extending about length of one segment. Typhlosole originating in xxvi, simple fold, 1/3 lumen diameter; intestinal wall with 35-39 longitudinal blood vessels. Hearts in x to xiii, esophageal; commissural vessels in vi, vii, and ix lateral; those in viii extend to gizzard.

Ovaries and funnels free in xiii. Single midventral spermatheca positioned on right side of the body, postseptal in vi, with nephridia on duct. Spermatheca with irregularly round ampulla; bulbous, muscular duct; two stalked diverticula attached ectally to duct, terminating in bean-shaped receptacle, stalk short. Male sexual system holandric, testes and funnels enclosed in paired sacs in x, xi; seminal vesicles xi, xii, each with digitate dorsal lobe; vesicles of xi enclosed in testis sac; vasa deferentia slender, free from body wall on way to ental end of prostatic ducts; prostates in xvii to xix (one individual has the prostates in xvi to xviii); each prostate a dense, racemose mass with three lobes; muscular duct entering anterior margin of copulatory bursa. Each copulatory bursa round in xviii; coelomic surface muscular, with single, muscular, banana-shaped secretory diverticulum projecting from the anterior margin; roof with thick pad, floor pads lacking; short, blocky penis present.

Remarks: Having a single midventral spermatheca indicates that Pheretima (Parapheretima) boaensis n. sp. belongs to the P. barbara group of Sims and Easton (1972). However, this species group is characterized by having a midventral spermatheca in 6/7 and 7/8. Sims and Easton (1972) assigned to this species group P. (Paraph.) barbara barbigua [replacement name for P. (Paraph.) barbara ambigua Cognetti 1913 by Blakemore (2004)]; P. (Paraph.) barbara barbara Cognetti 1913 ; and P. (Paraph.) hellwigiana Cognetti 1913. The new species differs from these species in having a single midvental spermatheca in 5/6. The shape of the secretory diverticula also differs; in the other species, the diverticula are more elongate, form kinks, and project from the posterior end of the copulatory bursae. Pheretima (Parapheretima) boaensis differs from P. (Paraph.) pandanensis n. sp. in the number and location of the spermathecae, distance between male pores, and projection of the secretory diverticula from the anterior end of the copulatory bursae.

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Figures

Fig. 3. Schematic dorsal views of the internal morphology of other Pheretima species described here. (A) P. timpoongensis n. sp., with intestinal origin in xv, and caeca extending from xxvii-xxiv; (B) P. camiguinensis n. sp., with prostates in xvii-xxi, and caeca extending from xxvii-xxi; (C) P. sibucalensis n. sp.; (D) P. apoensis n. sp. with spermathecae; (E) P. (Paraph.) pandanensis n. sp.; (F) P. (Paraph.) boaensis n. sp., with intestinal origin in xiv, prostates in xvii-xix, and caeca extending from xxvii-xxiii. Abbreviations: s, spermatheca; h, heart; p, prostate gland; cb, copulatory bursa; c, caecum. Scale bars: A-F, 5 mm.