Lopesia ubatubensis, Garcia & Urso-Guimarães, 2018

Garcia, Carolina de Almeida & Urso-Guimarães, Maria Virginia, 2018, Three new species of Lopesia Rübsaamen (Diptera: Cecidomyiidae) from Brazil, Florida Entomologist 101 (2), pp. 203-211 : 208-211

Lopesia ubatubensis sp. nov. Garcia & Urso-Guimarães ( Figs. 21–30 )

DESCRIPTION

Adult. Male – Light brown. Body: 1.7 mm long (male, n = 2); 1.8 mm long (female, n = 5). Head: Eyes black, holoptic, facets circular, closely adjacent. Occipital process present. Frontoclypeus with 5 long setae; labrum triangular with 3 pairs of setae; hypopharynx of the same shape as labrum; labella elongate-convex, each with 6 lateral setae; palpi total length, 0.3 mm, palpi 4-segmented. Antennae: Total length, 0.9 mm in male and 0.7 in female; scape and pedicel length, 0.2 mm; 12 binodal flagellomeres; tricircumfilar, circumfila whorls basal and distal long, regular in length and mesal circumfila appressed to flagellomere body ( Fig. 21 ); female flagellomeres cylindrical,with interconnected circumfila ( Fig. 22 ); setulose necks and apical process present ( Fig. 23 ); in both sexes. Thorax: Scutum and scutellum brown. Scutum with 1 row of dorsal setae and 1 row of lateral setae, anepimeron with 10 setae, katepisternum and anepistenum bare, laterotergite bare, mediotergite bare. Legs: First tarsomere without spur; tarsal claws toothed and bent near the base. Empodia reaching the bend in claws. Tarsal claws bent near midlength and toothed; empodia as long as the bend in claws ( Fig. 24 ). Wings (as in Fig. 5 ): 1.3 mm long and 0.5 mm wide (male); 1.5 mm long and 0.5 mm wide (female). Abdomen: Tergites 1–7 rectangular, sclerotized with a complete row of posterior setae in males and female; tergite 8 not sclerotized in males and female. Trichoid sensilla absent. Sternites 1–6 weakly sclerotized in males and females with a complete row of posteri- or setae.Sternites 7–8 not sclerotized in both sexes.Male terminalia ( Fig. 25 ): Gonocoxites elongated, narrow and with mesobasal lobe rounded and discrete; gonostylus clavate, bigger than gonocoxite, presence of several setae at lower margin, group of 6 sensillae at basal region; gonostylus teeth entire and discrete;cercus wide,bilobed with rounded apex; hypoproct deeply bilobed and setose, lobes apex rounded, same size as cercus, each lobe with some strong setae at tip; aedeagus narrow and elongated; rounded at apex and 1.5 longer than hypoproct; parameres absent. Ovipositor (as in Fig. 7 ): length, 0.15 mm; slightly protrusible, cerci separate, ovoid and setose, 2 setae longer than the others; hypoproct short and covered by setulae.

Pupa. Dark brown. Body: 1.9 mm long (n = 6). Head: Antennal horns, 0.1 mm long, triangular, dentate and sclerotized, presence of 3 asetose papillae on each horn (Fig. 26). Cephalic seta short (0.02 mm); 2 pairs of lower facial papillae, 1 asetose and 1 setose; 2 pairs of lateral facial papillae (1 asetose and 1 setose). Cephalic lateral spine rectangular. Thorax:Wing reaching second abdominal segment; foreleg, midleg, and hind leg reaching fourth abdominal segment. Prothoracic spiracle setiform and well developed (length: 0.23 mm). Abdomen: Abdominal tergites 1–8 completely covered with micro spines. Abdominal tergite 1 without dorsal spines. Abdominal tergites 2–6 with 1 row of con-

26–29. Lopesia ubatubensis sp. nov. (26). Pupal head (ventral and dorsal views). (27). Pupal abdominal tergites 6–9 with dorsal spines in the distal area. All tergites covered by micro spines as shown in the 5th segment. (28). Larval prothoracic spatula and lateral papillae. (29). Larval terminal segments.

spicuous spines varying in number. Spiracles present in dorsal tergites 2–6 and absent in tergites 7–9. Integument smooth. Terminal segment, 0.1 mm in length and width (Fig. 27).

Larva 3rd instar. Cream. Body length, 1.7 mm (n = 2). Spatula 2-toothed with long stalk, 0.1 mm long. Stalk strongly sclerotized in the upper and middle region, posterior region enlarged. Lateral papillae in 2 groups of 3 papillae, 2 setose and 1 asetose (Fig. 28). Terminal segment with 3 pairs of corniform papillae and 2 pairs of ventral papillae without setae, ventral anus in cleft (Fig. 29). Integument rough.

Types. HOLOTYPE male, Ubatuba, São Paulo, Brazil (Praia do Cedro, 23.5391°S, 45.1713°W),reared from leaf galls of Clidemia sp. ( Melastomataceae ), 26 Mar 2016 by Maria Virginia Urso-Guimarães, emerged 29 Jul 2016 (Museu de Zoologia of the Universidade of São Paulo, São Paulo, Brazil). PARATYPES, 1 male, 5 female, 2 pupal exuvia, 1 larva collected and reared with holotype (Museu de Zoologia of the Universidade of São Paulo, São Paulo, Brazil).

Etymology. The specific epithet refers to the type locality of the new species, the Ubatuba municipality.

Gall and biology. Globoid shape, green, red hairy, unilocular leaf gall in Clidemia sp. ( Melastomataceae ) ( Fig. 30 ). Tavares (1917) made references to a cecidomyiid as inducer of hairy, spherical galls on twigs and leaves of Clidemia sp. from Serra do CaraÇa, Minas Gerais State, Brazil. Despite the entire plant being collected, their specific identification was not possible because the plant was collected without the reproductive organs. Pupation occurs in the gall.

Remarks. Running the key to segregation of Lopesia species ( Rodrigues & Maia 2010), we observed that the specimens studied resemble Lopesia erythroxyli (Rodrigues & Maia) in the setulose flagellomere necks (couplet 1), narrow gonocoxites (couplet 2’), male flagellomere not gynecoid (couplet 7’); mesal circumfila with reduced circumfilar loops (couplet 9’); L. ubatubensis sp. nov. shares the narrow gonostylus with L. conspicua Maia, 2003 , and the short mesobasal lobe with L. similis Maia, 2004 ( Narahara et al. 2004; Madeira et al. 2003). Also it is similar in the developed pupal antennal horn (couplet 1), conspicuous apical setae of pupa, 0.02 mm long (couplets 2 and 3’), pupal dorsal abdominal spines present and simple (couplets 4 and 6) with L. spinosa Maia, 2004 ; and shares with L. caulinaris Maia, 2003 , the presence of larval prothoracic spatula with 2 teeth (couplets 1, 2’, and 3), terminal segment with weak reentrancy and with terminal papillae equally long (couplets 4’ and 5’), posterior margin of larval prothoracic spatula large and with sharp teeth spaced apart, with lateral process at apex (couplets 7’, 8’, and 9’), larval terminal segment with 2 lateral lobes at the posterior end (couplet 10) ( Madeira et al. 2003).

The following set of characteristics differentiates L. ubatubensis sp. nov. from its congeners: pupal antennal horns dentate and sclerotized with 3 papillae on each horn and cephalic lateral spine rectangular in pupae, male flagellomeres tricircumfilar; with mesal circumfila appressed to flagellomere; gonocoxites without medial constriction; and inducing galls in leaf of Clidemia sp.

Figures

Figs. 21–25. Lopesia ubatubensis sp. nov. (21). Third male flagellomere. (22). Third female flagellomere. (23). Twelfh male flagellomere. (24). Tarsal claws and empodia. (25). Male terminalia (dorsal view).

Fig. 30. Leaf galls of Lopesia ubatubensis sp. nov. in Clidemia sp. (Melastomataceae).

Figs. 1–7. Lopesia mataybae sp. nov. (1). Third male flagellomere (frontal view). (2). Third female flagellomere (frontal view). (3). Twelfh male flagellomere with apical process (frontal view). (4). Male tarsal claws and empodia. (5). Male wing. (6). Male terminalia (dorsal view). (7). Female postabdomen (lateral view).