Neopestalotiopsis piceana S.S.N. Maharachch., K.D. Hyde & P.W. Crous, Studies in Mycology 79:146. (2014)

Zhang, Zhaoxue, Liu, Rongyu, Liu, Shubin, Mu, Taichang, Zhang, Xiuguo & Xia, Jiwen, 2022, Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China, MycoKeys 88, pp. 171-192 : 171

Neopestalotiopsis piceana S.S.N. Maharachch., K.D. Hyde & P.W. Crous, Studies in Mycology 79:146. (2014)

Fig. 4

Description.

Leaf spots irregular, pale brown in centre, brown to tan at margin. Asexual morph on PDA: Conidiomata solitary, globose to clavate, semi-immersed, brown to black; exuding globose, dark brown to black conidial masses. Conidiophores reduced to conidiogenous cells. Conidiogenous cells discrete, ampulliform to lageniform, hyaline, smooth and thin walled, simple, 4.0-12.0 × 2.0-10.0 μm, apex 2.0-5.0 μm diam. Conidia ellipsoid to clavate, straight to slightly curved, 4-septate, 19.5-26.5 × 5.5-7.0 μm, mean ± SD = 22.7 ± 0.8 × 6.1 ± 0.4 μm; somewhat constricted at septa; basal cell obconic with truncate base, rugose and thin-walled, 2.7-5.0 μm long; three median cells 12.0-16.0 μm long, mean ± SD = 14.7 ± 0.9 μm, doliiform, verruculose, versicoloured, septa darker than the rest of the cell, second cell from base pale brown, 4.0-5.7 μm long; third cell dark brown, 3.5-5.2 μm long; fourth cell brown, 3.8-5.8 μm long; apical cell obconic, hyaline, thin and smooth-walled, 2.5-5.2 μm long; with 1-3 tubular apical appendages, arising from the apical crest, flexuous, unbranched, 21.0-32.0 μm long, mean ± SD = 24.8 ± 3.5 μm; basal appendage single, tubular, unbranched, centric, 2.7-6.5 μm long.

Culture characteristics.

Colonies on PDA incubated at 25 °C in the dark with an average radial growth rate of 9.0-14.0 mm/day and occupying an entire 90 mm petri dish in 7 d, with edge undulate, whitish, aerial mycelium on surface, fruiting bodies black, concentric; reverse of culture yellow to pale brown.

Specimen examined.

China, Hainan Province: Five Fingers Group Scenic Area, 20 May 2021, Z.X. Zhang. On diseased leaves of Ficus microcarpa , HSAUP210112, living culture SAUCC210112; on diseased leaves of Ficus microcarpa , HSAUP210113, living culture SAUCC210113.

Notes.

In the present study, two strains (SAUCC210112 and SAUCC210113) from symptomatic leaves of Ficus microcarpa were clustered with Neopestalotiopsis piceana clade ( Maharachch. et al. 2014) based on phylogeny (Fig. 1 ). Morphologically, our strains were the same as N. piceana , which was originally described with an asexual morph on wood of Picea sp., Cocos nucifera and fruit of Mangifera indica . The sexual morph of N. piceana was undetermined yet. Neopestalotiopsis piceana was a new record for China and first reported from Ficus macrocarpa ( Moraceae ).

Figures

Figure 1. Phylogram of Sporocadaceae based on combined ITS, tub 2 and tef 1 sequences. The BI and ML bootstrap support values above 0.90 and 70 % are shown at the first and second position, respectively. The tree is rooted to Bartalinia robillardoides (CBS 122705), ex-type or ex-epitype cultures are indicated in bold face. Strains from the current study are in red. Some branches were shortened according to the indicated mulipliers.

Figure 4. Neopestalotiopsis piceana (SAUCC 210112) a diseased leaf of Ficus microcarpa b surface of colony after 7 days on PDA c reverse of colony after 7 days on PDA d conidiomata e-g conidiogenous cells with conidia h-j conidia. Scale bars: 10 μm (e-j).