Colpoda inflata (Stokes, 1885), 1931

Kim, Kang-San, Ahn, Dong-Ha & Min, Gi-Sik, 2016, Record of two soil ciliates (Ciliophora: Colpodea: Colpoda) from Korea, Journal of Species Research 5 (3), pp. 463-467 : 465-466

2. Colpoda inflata (Stokes, 1885) , Kahl, 1931

ȁǖǐq섬AEệ (ṳAE) ( Table 1; Fig. 2 )

Material examined. Soil with twigs under a tree, Geonhwa­gil (Sarabong Park), Jeju­do province, South Korea (33°31′N 126°32′E), collected by Dong­Ha Ahn in November 2014 .

Diagnosis. Size 60­70 × 40­50 μm in vivo and 50­65 × 34­42 μm in impregnated specimens ( Table 1; Fig. 2A, B, D, I ). Distinctly wider postorally than preorally, like a letter “L” ( Fig. 2A, B, D ). Rigid and colorless at low magnification. Keel with 11­12 notches ( Fig. 1D, E ). Indistinct extrusomes, size approximately 1 × 0.5 μm in vivo ( Fig. 2F ). Cytoplasm colorless, food vacuoles approximately 5 μm in diameter, and lipid drops irregularly distributed ( Fig. 2F ). One macronucleus 10 × 9 μm on average ( Table 1; Fig. 2A, J ). Contractile vacuole at posterior end ( Fig. 2A, E ). Typical Colpoda ciliature pattern; 22­28 somatic kineties, 15­20 left polykinetids, and 6­8 postoral kineties ( Table 1; Fig. 2B, I ). Silverline system cucullus ­ type ( Fig. 2K ).

Remarks. Colpoda inflata is easily distinguished from the other Colpoda species because it has a unique body shape, like the letter “L” ( Fig. 2A, B, D ) ( Foissner, 1993). Among the Colpoda species, Colpoda augustini Foissner, 1987 and C. maupasi Enriques, 1908 have body size similar to C. inflata ( Foissner, 1993) .

Colpoda augustini differs from C. inflata in the following features: body shape (reniform vs. “L” shape); length of preoral portion (6­9 μm vs. 15-18 μm); and number of somatic kineties (15­19 vs. 22­28) ( Table 1; Foissner, 1993).

Colpoda maupasi can be separated from C. inflata by body shape (reniform vs. “L” shape), length of preoral portion (8­11 μm vs. 15-18 μm), number of somatic kineties (15­18 vs. 22­28), and silverline system (aspera ­ type vs. cucullus ­ type) ( Table 1; Foissner, 1980).

Habitat. Soil with twigs under a tree.

Distribution. USA, China, Central Europe, Sweden, Japan, Africa, Mexico, New Zealand, Australia, Korea.

Deposition. Two voucher slides with protargol­impregnated specimens are deposited in the National Institute of Biological Resources in Korea (NIBRPR0000107170, NIBRPR0000107171).

Identifiers. Kang­San Kim and Gi­Sik Min.

Figures

Fig. 2. Colpoda inflata from live (A, D­H), protargol­impregnated (B, C, I, J) and silver nitrate­impregnated (K) specimens. A, B, D, E, I. right lateral views. C, J. left lateral views. C. arrow indicates excretory pore. D. arrow indicates notches. E. arrow marks contractile vacuole. F. extrusome at the cell margin (arrows), arrowheads indicate food vacuoles. G. right oral polykinetid (arrow) and left oral polykinetid (arrowhead). J. arrow marks excretory pore. K. “Cucullus­type” silverline system, arrows indicate granules. CV, contractile vacuole. lP, left oral polykinetid. Ma, macronucleus. Mi, micronucleus. rP, right oral polykinetid. Scale bars: 50μm.

Fig. 1. Colpoda cucullus from live (A, D­G), protargol­impregnated(B, C, H, I) and silver nitrate­impregnated(J) specimens.A, B, D, E, H. right lateral views. C, I. left lateral views. C. arrow indicates excretory pore. E. arrow marks contractile vacuole, arrowhead indicates notches. F. extrusome at the cell margin (arrows). I. arrow marks excretory pore. J. “Cucullus­type” silverline system, arrows indicate granules. CV, contractile vacuole. lP, left oral polykinetid. Ma, macronucleus. Mi, micronucleus. rP, right oral polykinetid. Scale bars: 50μm.