Cernosvitoviella aggtelekiensis Dózsa-Farkas, 1970

Dózsa-Farkas, Klára, Csitári, Bianka & Felföldi, Tamás, 2017, A new Cernosvitoviella species (Clitellata: Enchytraeidae) and its comparison with other Cernosvitoviella species from Sphagnum mires in Hungary, Zootaxa 4254 (3), pp. 322-338 : 328

Cernosvitoviella aggtelekiensis Dózsa-Farkas, 1970

( Figures 1 B, 5–6)

Cernosvitoviella aggtelekiensis Dózsa-Farkas, 1970: 247 –250, fig. 3, +5 photos. Cernosvitoviella goodhui Healy, 1975 . Syn: Schmelz & Collado 2010: 61. nec: Cernosvitoviella goodhui Healy, 1975 . Chalupský, 1992: 141. fig. 8. nec: Cernosvitoviella cf. goodhui Healy, 1975 . Rota 1995: 193 –194.

Material investigated: (in total 11 specimens), 8 specimens from site 7 (31.03.2014 and 16.05.2015), 1 specimen from site 8 (31.03.2014), 2 specimens from site 5 (27.03.2016).

Body 1.7–3 mm long, 180–240 µm wide at the clitellum, segments 21–23. Chaetae sigmoid with nodulus, up to 7–9 per bundle, 29–32 µm long ( Figs. 5 C,D). Clitellum absent dorsally and ventrally. Only dark spindleshaped coelomocytes, slightly wider than in C. farkasi sp. n., 35–57 µm long and 9–10 µm wide in the middle ( Figs. 5 E,F). In the prostomium, about 8 large rounded papillae are observable ( Figs. 5 A,B). Two or three paired or unpaired nephridia preclitellarly, from 6/7 ( Fig. 5 H). Seminal vesicle absent. Chloragogen cells 20–30 µm long ( Fig. 5 G). Sperm funnel cylindrical, 45–70 µm long and about 2–3 times longer than wide. The collar tall, as wide as the body of funnel ( Figs. 5 I,J, 6B). Sperm duct similar to C. farkasi sp. n. with a large dilatation ( Figs. 5 I,J), but not as conspicuous and somewhat narrower (20–30 µm and 8–15 µm wide, vs. 30–50 µm and 17–26 µm in vivo in C. farkasi sp. n., respectively), moreover slightly closer to the male opening than in the new species. Male opening surrounded by glands, diameter about 20–30 µm ( Figs. 6 A,C). Length of spermatozoa 35–40 µm, heads 15–20 µm ( Fig. 6 B). Spermathecae ( Figs. 1 B, 6D–F) extending into V–VI, ectal ducts (50–80 µm long and 8–9 µm wide), widened at the ectal orifice into 15–19 µm, no glands at the openings. Ampullae oval (50–70 µm long and 15–19 µm wide), filled with sperm.

Figures

FIGURE 1. Spermathecae of Cernosvitoviella species. A. C. farkasi sp. n. B. C. aggtelekiensis. C. C. atrata. D. C. crassoductus. E. C. minor s. l. with small spermatheca. F. C. minor s. l. Scale bars 50 µm.

FIGURE 5. Micrographs of Cernosvitoviella aggtelekiensis. A. Prostomial papillae (arrows). B. Prostomial papillae in 100 x magnification (black arrows, coelomocytes marked with white arrow). C. Chaetae. D. Chaetae in 100 x magnification. E. Coelomocytes outside the body. F. Coelomocytes in coelom. G. Chloragogen cells in 100 x magnification. H. The second pair of nephridia in 7 / 8 (arrow). I – J. Sperm funnels (white arrows), dilation of the sperm ducts (black arrows), e = eggs. All micrographs in vivo. Scale bars in A, C, E – F, H – J 50 µm, in B, D, G 20 µm.

FIGURE 6. Micrographs of Cernosvitoviella aggtelekiensis. A. Dilatation (white arrow) and last part of the sperm duct with the male opening (black arrow, e = egg in 100 x magnification). B. Sperm funnel in 100 x magnification. C. Male pores with glands surrounding (arrow) in 100 x magnification. D – F. Spermathecae (arrows, p = primary pharyngeal glands, sp = secondary pharyngeal glands). All micrographs in vivo. Scale bars in D – F 50 µm, in A – C 20 µm.