Rhizobium alamii BT

Han, Joo Hyun, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Lee, Byoung-Hee & Kim, Ki-eun Lee and Myung Kyum, 2020, Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea, Journal of Species Research 9 (2), pp. 85-104 : 101-102

Description of Rhizobium alamii BT 338

Cells are Gram-stain-negative, non-flagellated, and rod-shaped. Colonies are white-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III positive for D- maltose, D- trehalose, D- cellobiolose, gentiobiose, sucrose, D- turanose, pH 6, α -D- lactose, D- melibiose, N -acetyl-D- glucosamine, N -acetyl- β -mannosamin, 1% NaCl, α - D- glucose, D- mannose, D- fructose, D- galactose, L- fucose, L- rhamnose, 1% sodium lactate, D- mannitol, D- arabitol, myo-inositol, glycerol, glycerol, rifamycin SV, L- glutamic acid, lincomycin, quinic acid, tetrazolium violet, tetrazolium blue, methyl pyruvate, L- malic acid, nalidixic acid, potassium tellurite and acetic acid; weak positive for dextrin, D- raffinose, β -methyl-D- glucoside, D- salicin, N - acetyl-D- galactosamin, N -acetyl N -acetyl neuraminic acid, D- fucose, D- sorbitol, glycyl-L- proline, L- alanine, L- aspartic acid, L- histidine, L- serine, pectin, D- glucuronic acid, D- malic acid, bromo-succinic acid, acetoacetic acid, formic acid and aztreonam; but negative for stachyose, pH 5, 4% NaCl, 8% NaCl, 3-methyl-glucose, inosine, fusidic acid, D- serine, D- glucose-6-PO 4, D- fructose-6-PO 4, D- aspartic acid, D- serine, minocycline, gelatin, L- arginine, L- pyroglutamic acid, guanidine HCl, niaproof 4, D- galacturonic acid, L- galactonic acid lactone, D- gluconic acid, glucuronamide, mucic acid, D- saccharic acid, vancomycin, p -hydroxy-pheylacetic acid, D- lactic acid methyl ester, L- lactic acid, citric acid, α -keto-glutaric acid, lithium chloride, tween 40, γ -amino-butryric acid, α -hydroxy-butyric acid, β -hydroxy-D, L- butyric acid, α -keto butyric acid, propionic acid, sodium butyrate and sodium bromate. In API 20NE test, positive for esculin hydrolysis; weak positive for β -galactosidase; but negative for reduction of nitrates (NO 3) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, arginine dihydrolase, urease, gelatin hydrolysis. D- Glucose L- arabinose, D- mannose, D- mannitol, D- maltose and malic acid, N - acetyl-D- glucosamine (weak) were utilized; but potassium gluconate, capric acid, adipic acid, trisodium citrate and phenylacetic acid were not utilized. In API 32 GN test, glycogen, salicin, D- fucose, D- sorbitol, 4-hydroxybenzoic acid, L- rhamnose, N -acetyl-glucosamine, inositol, D- saccharose (sucrose), D- maltose, L- serine, D- mannitol, salicin and D- sorbitol were utilized whereas, D- ribose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L- alanine, potassium 5-ketogluconate, 3-hydroxybenzoic acid, L- serine, propionic acid, capric acid, valeric acid, trisodium citrate, L- histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid and L- proline were not utilized.

Strain BT338 (= NIBRBAC000503035) was isolated from a soil sample, Uijeongbu City in Gyeonggi Province, Korea.