Pseudomonas reidholzensis BT

Han, Joo Hyun, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Lee, Byoung-Hee & Kim, Ki-eun Lee and Myung Kyum, 2020, Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea, Journal of Species Research 9 (2), pp. 85-104 : 99-101

Description of Pseudomonas reidholzensis BT 362

Cells are Gram-stain-negative, flagellate, and rod-shaped. Colonies are yellow-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, positive for pH 6, pH 5, 1% NaCl, 4% NaCl, α - D- glucose, D- mannose, D- fructose, 1% sodium lactate, fusidic acid, glycerol, troleandomycin, rifamycin SV, L- alanine, L- arginine, L- aspartic acid, L- glutamic acid, L- histidine, L- serine, lincomycin, guanidine HCl, niaproof 4, D- galacturonic acid, D- gluconic acid, D- glucuronic acid, mucic acid, quinic acid, vancomycin, tetrazolium violet, tetrazolium blue, L- lactic acid, citric acid, L- malic acid, nalidixic acid, lithium chloride, potassium tellurite, γ -amino-butryric acid, β -hydroxy-D, L- butyric acid, propionic acid and acetic acid; weak positive for 8% NaCl, inosine, D- serine, D- fructose-6-PO 4, glyctl-L- proline, L- galactonic acid lactone, glucuronamide, D- saccharic acid, methyl pyruvate, α -keto-glutaric acid, bromo-succinic acid, α -keto butyric acid, formic acid and aztreonam; but negative for dextrin, D- maltose, D- trehalose, D- trehalose, gentiobiose, sucrose, D- turanose, stachyose, D- raffinose, α - D- lactose, D- melibiose, β -methyl-D- glucoside, D- salicin, N -acetyl-D- glucosamine, N -acetyl- β -mannosamin, N -acetyl-D- galactosamin, N -acetyl N -acetyl neuraminic acid, D- galactose, 3-meth-

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yl-glucose, D- fucose, L- fucose, L- rhamnose, D- sorbitol, D- mannitol, D- arabitol, myo-inositol, D- glucose-6-PO 4, D- aspartic acid, D- serine, minocycline, gelatin, L- pyroglutamic acid, pectin, p -hydroxy-pheylacetic acid, D- lactic acid methyl ester, D- malic acid, tween 40, α -hydroxy-butyric acid, acetoacetic acid, sodium butyrate and sodium bromate. In API 20NE test, positive for β -galactosidase; weak positive for esculin hydrolysis; but negative for reduction of nitrates (NO 3) to nitrite (NO 2 -), reduction of nitrates (NO 3) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, arginine dihydrolase, urease, gelatin hydrolysis. D- Mannitol and D- maltose, D- glucose (weak), L- arabinose (weak), D- mannose, potassium gluconate (weak), adipic acid (weak) and malic acid (weak) were utilized whereas, N - acetyl-D- glucosamine, capric acid, trisodium citrate and phenylacetic acid were not utilized. In API 32GN test, L- alanine, L- serine, D- glucose, propionic acid, capric acid, valeric acid, trisodium citrate, L- histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4-hydroxybenzoic acid, L- proline, D- ribose, sodium malonate, sodium acetate, lactic acid and 3-hydroxybenzoic acid were utilized whereas, L- rhamnose, N -acetyl-glucosamine, inositol, D- saccharose (sucrose), D- maltose, itaconic acid, suberic acid, potassium 5-ketogluconate, glycogen, potassium 5-ketogluconate, glycogen, D- mannitol, salicin, D- melibiose, D- fucose, D- sorbitol and L- arabinose were not utilized.

Strain BT362 (= NIBRBAC000503041) was isolated from a soil sample, Seogwipo City in Jeju Island, Korea.