Paracoccus onubensis, Gutierrez-Patricio & Gonzalez-Pimentel & Miller & Hermosin & Saiz-Jimenez & Jurado, 2021

Gutierrez-Patricio, Sara, Gonzalez-Pimentel, Jose L., Miller, Ana Zelia, Hermosin, Bernardo, Saiz-Jimenez, Cesareo & Jurado, Valme, 2021, Paracoccus onubensis sp. nov., a novel alphaproteobacterium isolated from the wall of a show cave, International Journal of Systematic and Evolutionary Microbiology (004942) 71 (8), pp. 1-10 : 8

DESCRIPTION OF PARACOCCUS ONUBENSIS SP. NOV.

Paracoccus onubensis (o.nu′ ben.sis, N.L. masc. adj. onubensis , pertaining to Onuba, the Roman name of Huelva, the province where the organism was first isolated).

Cells are facultatively anaerobic, non-motile, Gram-stainnegativeandnon-endospore-forming.Theyare 0.5–0.7µm×1.2– 1.8µm in diameter, grow in pairs or singly (Fig. S4). Colonies on marine agar are 0.5–1.3mm in diameter, circular, convex, smooth and have entire margins. Good growth at 10–37°C, optimal at 20–30°C and weak growth at 8 and 42°C. Tolerates up to 12% NaCl, with optimum growth within 3–5% (v/w) NaCl. Growth occurs at pH 5.0–9.0, with an optimum at pH 5.0–6.0. Catalase- and oxidase-positive. It grows heterotrophically with various carbon sources and chemoautotrophically with thiosulfate under aerobic conditions. D-Glucose,L-galactose, D -mannose,D-mannitol, N -acetylglucosamine, maltose, potassium gluconate, adipate, malate, citrate and phenylacetate can be utilized as carbon sources. Utilizes sodium nitrate, ammonium sulphate, sodium glutamate, casamino acids and peptone as sole nitrogen sources. Produces acid from D,L-arabinose, aesculin, D -fructose, D-fucose, D-galactose, D-glucose, D-mannose, D-ribose andD,L-xylose; weakly from D-adonitol,L-arabitol, L-fucose, glycerol, D-lyxose, maltose, melibiose, sucrose and trehalose. Negative for amygdalin, D-arabitol, arbutin, dulcitol, gentibiose, glycogen, inositol, inulin, lactose, melezitose, methyl α-D-glucopyranoside, methyl α-D-mannopyranoside, methyl β- D-xylopyranoside, N -acetylglucosamine, potassium gluconate, potassium 2-ketogluconate, potassium 5-ketogluconate, raffinose, L-ramnose, salicin, D-sorbitol, L-sorbose, starch, D -tagatose, turanose and xylitol. Reduces nitrate to nitrite. Hydrolyses aesculin and Tween 20, and does not hydrolyse DNA, casein or Tweens 40 and 80. Produces alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, naphthol- AS-BI-phosphohydrolase and α-glucosidase. Assimilates glucose, arabinose, mannose, mannitol, N -acetyl-glucosamine, maltose, potassium gluconate, adipic acid, malate, trisodium citrate and phenylacetic acid, but does not assimilate capric acid. The most abundant fatty acid is C 18:1 ω7 c. Ubiquinone-10 is the major respiratory quinone. The polar lipids are diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and an unidentified aminolipid, an unidentified glycolipid and unidentified polar lipid.

The G+C content of the type strain is 60.3 mol%. The type strain, 1011MAR3 C25 T (=CECT 9092 T =LMG 29414 T), was isolated from white biofilms on the walls of the cave Gruta de las Maravillas , Aracena (Huelva, Spain).