Cyrenoida implexa, Valentas-Romera & Simone & Marques, 2024

Valentas-Romera, Bárbara L., Simone, Luiz Ricardo L. & Marques, Rodrigo Cesar, 2024, A new species of Cyrenoida (Bivalvia, Cyrenoididae) from the Western Atlantic, with remarks on Cyrenoididae anatomy, Zoosystematics and Evolution 100 (2), pp. 543-553 : 543-553

Cyrenoida implexa sp. nov.

Figs 1 – 7 , 8 – 12 , 13 – 16 , 17–23

Diplodonta punctata : Barroso and Matthews-Cascon 2009: 82–83 (non Say, 1822).

Cyrenoida sp.: Rodrigues et al. 2006: 395, 397; Huber 2015: 812; Saad et al. 2019: 5 – 6.

Types.

Holotype: BRAZIL • specimen; MZSP 54637 . Paratypes: 40 specimens; same locality as holotype; Barroso C. X. leg.; 17 Oct. 2005; MZSP 99988 .

Type locality.

BRAZIL. Ceará; Fortaleza, Ceará River estuary, Parque Soledade, 3 ° 42 ' 07.94 " S, 38 ° 36 ' 36.22 " W, IX Martins leg., 17. x. 2005.

Extra material examined.

BRAZIL • 1 specimen; Maranhão, São Luís, São Marcos Bay, Carangueijos Island , Igarapé mangrove; 2011; MZPS 100525 . 2 specimens: São Paulo, São Vicente , Branco River mangrove; 23 ° 56 ' 17 " S, 46 ° 25 ' 12 " W; 2 Jul. 2011; Saad, L. O. leg.; MZSP 109103 . 06 specimens; 08 Feb. 2012; Saad, L. O. leg; MZSP 109105 . 2 specimens: Peruíbe , Una River mangrove, Ecological Station Juréia Itatins ; 24 ° 25 ' 33 " S, 47 ° 05 ' 05 " W; 03 Apr. 2012; Saad, L. O. leg; MZSP 109104 .

Measurements

(length, height, and maximum width in mm). MZUSP 54637: 7.2 by 7.3 by 4.3; MZSP 99988 # 1: 7.1 by 6.9 by 4; # 2: 8.2 by 8.3 by 4.5; # 3: 6.7 by 6.6 by 3.6; # 4: 6.7 by 6.2 by 3.4; # 5: 4.9 by 4.6 by 2.9; # 6: 5.4 by 5 by 3.1; # 7: 6.8 by 6.3 by 3.4; # 8: 6.1 by 5.5 by 3.3; # 9: 5.5 by 5.3 by 3.3; MZSP 100525: 7.7 by 7.2 by 4.1.

Diagnosis.

Shell rounded to subquadrate, posteriorly pointed; umbones high, thin; valves fragile; periostracum thin, light brown. Internal surface opaque; no nacreous aspect; no distinguishable muscular impression or pallial line. Hinge with laminar cardinal and lateral teeth; right hinge with inverse V-shaped tooth, formed by fusion between cardinal and lateral tooth; laminar, lateral tooth; left valve with recumbent F-shaped teeth, formed by fusion of two cardinal teeth and lateral tooth. Nymph is long and thin.

Description.

Shell (Figs 1 – 7 ): Outline rounded to subquadrate, with ventral margin slightly posteriorly pointed. Width ~ 55 % of shell length. External surface covered with well-marked growth lines. Equivalve, almost equilateral, ~ 5 % longer than high, reaching maximum length of ~ 8 mm. Laterally inflated, width ~ 55 % of total shell length. Externally white, adorned only by growth lines. Periostracum thin, light brown; slightly wrinkled at ventral shell margin (Figs 1 , 2 ). Walls thin, fragile. Umbones central, prosogyre, low, ~ 6 % of total shell height and ~ 27 % of shell length, located almost at midpoint of shell length (Fig. 5 ). Internal surface opaque (Figs 3 , 4 ). Muscle scars and pallial line almost imperceptible. Anterior adductor muscle scar reniform, occupying ~ 1.32 % of total internal surface; twice higher than wide, located at mid third of shell height; Posterior adductor muscle scar oval, occupying ~ 1.49 % of total internal surface; located at mid-third of shell height. Pallial line entire, away from shell margins ~ 8 % of shell height. Hinge heterodont (Figs 6 , 7 ): right valve with inverse V-shaped tooth (Fig. 7 : lv), looking fusion between short cardinal tooth and long lateral tooth, with ~ 1 % of total shell length and single laminar lateral tooth (Fig. 7 : t 3) located under inversed V-shaped tooth, ~ 38 % longer than superior lateral tooth, forming groove between superior and inferior lateral teeth. Left valve with recumbent F-shaped tooth (Fig. 6 : fs), forming ~ 90 ° angle, with bifid appearance, looking fusion of two cardinal tooth (t 5, t 6) at one lateral tooth (t 4). Dorsal margin concave. Ligament parvincular, opisthodetic, length ~ 40 % of total shell length. Nymph ~ 9 times longer than wide, shape rhomboid. Lunule and escutcheon absent.

Main muscle system (Figs 8 , 9 , 13 ): Anterior adductor (aa) muscle reniform in transverse section, twice heigh than wide; ventral portion ~ 2.5 times wider than dorsal portion; occupying ~ 5 % of total internal shell volume; located at median third of valve, clearly divided into quick and slow components, quick component occupying ~ 30 % of anterior portion of muscle, dark grey in color, slow component occupying ~ 70 % of posterior portion of muscle, light cream in color. Posterior adductor muscle (pa) elliptical in cross section, ~ 1.5 times wider than tall, ~ 30 % shorter and ~ 1.3 times wider than anterior adductor muscle, located at opposite extremity of anterior adductor muscle, clearly divided into quick and slow components, the former occupying ~ 45 % of posterior portion of muscle, dark gray in color, the latter occupying ~ 55 % of anterior portion of muscle, light cream in color. Pair of foot anterior retractor muscles (ar) oval in section, thin, elongated; originated dorsally at anterior adductor muscle; running posteriorly and ventrally at ~ 20 % of total shell length; both branches fusing at anterior edge of foot base. Pair of foot posterior retractor muscled (pr) oval in section, thin; originated dorsally at posterior adductor muscle; ~ 50 % longer than pair of anterior retractors; both fusing posterior edge of foot base (Fig. 11 ). Two pairs of siphonal retractor muscles; dorsal siphonal retractors (dm) ~ 6 times longer than wide; insertion almost at central portion of mantle lobe, 2 times as long as excurrent opening, originating laterally at half of siphonal base height; ventral siphonal retractors (vm) thin, ~ 5 times longer than wide, length ~ 65 % of total length of dorsal siphonal muscle, originating at ventral end of inhalant opening.

Foot and byssus (Figs 8 , 11 ): Foot (ft) short; relaxed length ~ 50 % of total shell height. Laterally flattened; end blunt, swollen. Foot base at median portion visceral sac. Byssus or byssal groove.

Mantle (Figs 8 , 9 , 11 ): Mantle lobes symmetrical, thin, translucent, colorless. Pallial muscles (pm) reunited in long muscles, distributed sparsely along ventral side of mantle lobe; height ~ 12 % of total shell length, separated from each other by ~ 15 times pallial muscle basal width. Mantle edge trifolded (Fig. 9 ), unpigmented; outer fold (of) thick, ~ 7 times taller than wide; middle fold (mf) short, half of total outer fold height, same width; inner fold (if) short, length ~ 20 % of outer fold height, ~ 30 % of its width. Periostracum produced between external and middle fold. Mantle lobes totally free except for siphonal area. Anterior mantle fusion occurring at ~ 45 % of anterior adductor muscle height; posterior mantle fusion occurring at ~ 68 % of posterior adductor muscle height (Fig. 11 ). Siphonal area corresponding to ~ 42 % of total mantle lobe length. Mantle lobes mostly free from each other, except for siphonal area, relative to 42 % of mantle lobe total length (more details below).

Pallial cavity (Figs 8 , 10 – 14 , 16 ): Occupying about half of inner shell volume. Palps small, occupying ~ 30 % of total shell volume. Pair of hemipalps triangular (Figs 11 , 16 ), ~ 20 % shorter but same width of insertion area of anterior adductor muscle; pair of external hemipalps (op) connected at mantle lobe by hemipalp dorsal border, in half of hemipalp length; pair of internal hemipalps (ip) connected at visceral mass by dorsal border, in ~ 30 % of total hemipalp length. Internal surface of both palps covered by ~ 24 transverse folds; internal hemipalp folds high and rounded, covering ~ 90 % of hemipalp internal surface, forming thin smooth area at hemipalp borders corresponding ~ 1 % of total hemipalp internal area, folds decreasing towards mouth, forming shallow channels towards mouth (mo). Gills area ~ 30 % of total valve area; outer demibranch (od) fusiform, twice longer than wide, folded on ~ 30 % of total demibranch extension, covering pericardium and kidney areas, connected to mantle lobe by ~ 15 % of total length of dorso posterior border of demibranch. Inner demibranch (id) triangular, ~ twice longer than wide, folded on half of demibranch total extension, ~ 40 % of internal demibranch area covered by external demibranch (Fig. 8 ); presenting food groove (fg); demibranchs connected to each other by tissue at posterior end, in ~ 20 % of total gill length (Fig. 12 ). Suprabranchial chamber volume ~ 60 % of infrabranchial chamber volume. Incurrent (is) and excurrent (ex) siphons originated by inner mantle fold; length ~ 30 % of total shell length; each one ~ 3 times longer than wide in retracted condition; externally fused with each other; internally separated by thick, smooth muscular wall. Inner siphonal openings directly to pallial cavity (Figs 11 , 14 ). Incurrent siphon length ~ 20 % of total shell length, height ~ 1 % of total shell height. Excurrent siphon length ~ 80 % of inhalant siphon length; same width. Distal opening of incurrent siphon flanked by three rows of papillae (Fig. 14 : ir, mr, or); papillae length equivalent ~ 5 % of total inhalant siphon length. Distal opening of excurrent siphon with single row of flattened papillae (Fig. 14 : 9 p). Siphon attached to mantle by 2 pairs of muscle described above.

Visceral mass (Fig. 11 ): Visceral sac occupying half of inner shell volume; shape triangular. Slightly flattened; twice wider than muscular base; located dorsally at foot retractor muscles; ~ 20 % of anterior portion filled with digestive diverticula of brown color; remaining areas with gonad of cream color. Stomach and style sac located vertically at central region.

Circulatory and excretory system s (Figs 11 , 15 , 23 ): Pericardium located dorsally in posterior half of visceral sac, between posterior portion of umbonal cavity and dorsal surface of kidney; twice longer than wide; occupying ~ 25 % of total visceral sac volume. Pair of auricles (au) antero posteriorly long; connected to central axis of gill in ~ 30 % of total auricle length; walls thin, translucent walls thin; located at central portion of pericardium; surrounding ~ 50 % of intestinal portion crossing pericardium; connected to auricles in median portion of lateral walls. Kidney (ki) located postero ventrally at visceral mass (Figs 11 , 15 ), below posterior end of pericardium and dorsal surface of posterior foot retractor muscles, color light brown; shape triangular, occupying ~ 25 % of visceral mass volume. Gonopore (gp) rounded, located at posterior portion of visceral mass, at ~ 25 % of visceral mass height, opening in suprabranchial chamber, next to nephropore (np).

Digestive system (Figs 16 , 20 – 22 ): Palps and digestive diverticula described above. Mouth small, located in central portion at intersection of palps (Fig. 16 ), lips small. Esophagus (es) elongate, narrow, cylindric; length and height respectively ~ 16 % and 1 % of total visceral sac; no contact with anterior adductor muscle; passing through anterior portion of foot anterior retractors; internal surface with low longitudinal folds (fo); low esophageal rim (er) at stomach entrance; stomach (Figs 20 – 21 : st) occupying ~ 25 % of total visceral sac volume; shape elliptical, funnel-like; located slightly posteriorly to umbones; length ~ 80 % of total visceral sac length, ~ 30 % of visceral sac heigh; posterior portion ~ 60 % wider than anterior portion. Paired apertures to digestive caeca located ventro-laterally of gastric anterior portion, in transition with esophagus; turned to ventral side of visceral sac. Dorsal hood (dh) narrow, thin, length ~ 25 % of total stomach length, distally pointed. Left pouch (lp) located below anterior portion of dorsal hood, anteriorly to connection of digestive diverticula; shallow and wide; occupying ~ 20 % of total area of left external wall of stomach. Internal surface of stomach (Fig. 22 ) mostly smooth, covered by three sorting areas well defined. First sorting area starting at left side of esophageal rim, running along dorsal wall of anterior stomach chamber, penetrating dorsal hood, narrow, comprised of small transverse folds (sa 1). Second sorting area originating ventral to first sorting area, at left side of esophageal rim, running along left wall of anterior stomach chamber, entering left pouch and dorsal hood, both on their ventral surfaces, broad, formed by thickening of stomach wall (sa 2). Third sorting area starting inside dorsal wall of dorsal hood, running along dorsal and right walls of posterior stomach chamber, until diffusing on ventral portion of right wall (sa 3). Gastric shield (gs) located at central dorsal wall, occupying ~ 30 % of total gastric area, with two anterior projections, one dorsal at left border, penetrating dorsal hood, and one left ventral, penetrating left pouch. Two narrow, tall gastric ridges running along ventral stomach chamber, forming major and minor typhlosoles at style sac opening. Longer ridge originating at ventral surface of stomach, surrounding left digestive diverticula, penetrating style sac at its right side, forming major typhlosole (mt). Shorter fold originating at style sac entrance, at region of major typhlosole penetration into style sac, forming rim bordering style sac entrance and ultimately minor typhlosole (nt). Style sac (ss) connecting ventrally to dorsal portion of stomach; conical; tapering in ventral surface of visceral sac; ~ 3.5 times longer than wide; occupying ~ 16 % of total visceral sac volume; height equivalent to half of visceral sac total length, length ~ 1 % of visceral sac length. Intestine (in) narrow, long; starting in style sac; performing an inverted U-shaped loop under the anterior portion of the stomach, reaching the style sac high (Fig. 20 ), running towards dorso-posterior region of visceral sac parallel to style sac; leaving posterior portion of visceral sac, crossing pericardium and kidney; passing between posterior ends of foot posterior retractor muscles. Flanking entire posterior surface of posterior adductor muscle. Anus (an) on ventral surface of posterior adductor muscle; intestine total length ~ 9 times longer than style sac. Anus simple, sessile.

Genital system (Figs 11 , 15 ): Gonads described above. Pair of gonoducts receiving sort of gonad acini along length along anterior portion of visceral sac. Genital pore simple, located at posterior region of visceral sac (Fig. 11 : gp), opening next to nephropore (Figs 11 , 15 : np).

Central nervous system (Figs 17 – 19 ): Pair of cerebral ganglia (Fig. 17 : cg) surrounding anterior dorsal half of esophagus, dorsally to labial palps; shape slightly triangular; longer than wide; size ~ 50 % of esophagus width; cerebral commissure (cc) length ~ 60 % of each ganglion length; from anterior portion connecting anterior adductor muscle nerve, bifurcating in two branches, internal branch penetrating dorso posterior third of muscle and leaving at ventral surface of muscle; other branch bordering posterior surface of anterior muscle, both branches fusing at ventral region of anterior muscle; two connectives originating dorsally in ganglia, anteriorly to cerebro-visceral connective crossing visceral mass, touching gonopore dorsally, bordering anterior portion of kidney and connecting dorsally at visceral ganglia, connecting posteriorly cerebro-pedal connective running immersed at pedal muscles, connecting to anterior region of pedal ganglia. Pair of visceral ganglia (Fig. 19 : vg) fusiform; each ganglion slightly longer than wide; ~ 80 % of cerebral ganglia size; partially fused at median portion, with presence of shallow central groove; located ventrally at kidney, parallel to posterior adductor muscle; in dorsal tip connecting cerebra-visceral connective and renal nerve, penetrating into kidney area; laterally originating ctenidial nerves running thought central axis of posterior portion of gills; dorsally originating posterior adductor muscle nerve, penetrating median region of anterior surface of posterior muscle; at ventral tip originating pallial nerve, touching anterior surface of ventral portion of posterior adductor muscle, running parallel to inhalant and exhalant apertures and mantle border, diffusing at mantle lobe board. Pair of pedal ganglia (Fig. 18 : pg) 40 % larger than pair of cerebral ganglia; shape elliptic; longer than wide; totally fused with each other, without vestigial commissure; located immerse on foot retractor muscles, above foot insertion; in anterior tip connects cerebro-pedal connectives from cerebral ganglia; in posterior tip connecting two pairs of nerves, dorsal pair running towards posterior region inside posterior foot retractor muscles; postero-ventral nerves curved to ventral region, running internally.

Etymology.

The specific epithet implexa is a Latin word for “ tangled, ” referring to species commonly found between the roots of estuarine and mangrove plants.

Distribution.

Brazil, Maranhão to São Vicente, São Paulo (Fig. 24 ).

Habitat.

Mangroves in brackish water, buried until 15 cm in muddy sand.

Figures

Figure 24. Cyrenoida implexa distribution along the Brazilian coast.

Figures 1 – 7. Holotype of Cyrenoida implexa sp. nov. (MZSP 54637, 7.2 mm, H 7.3 mm, W 4.3 mm). 1. Left valve, outer view; 2. Right valve, outer view; 3. Left valve, inner view; 4. Right valve, inner view; 5. Whole dorsal view; 6. Right hinge under SEM; 7. Left hinge under SEM. Scale bar: 200 μm (6, 7).

Figures 8 – 12. Cyrenoida implexa, anatomical drawings. 8. Right view, valve removed, structures seen by transparency of mantle lobe; 9. Mantle border, transverse section in its ventromedial portion; 10. Gill, transverse section in its central portion; 11. Right view, right mantle, and gill removed; 12. Postero-ventral visceral region, ventral view, showing fusion of inner demibranchs in siphonal base and mantle fusion at siphonal area. Scale bars: 2 mm.

Figures 13 – 16. Cyrenoida implexa anatomical drawings. 13. Incurrent and excurrent siphons, anterolateral view, right mantle lobe partially removed, some adjacent structures shown; 14. Siphon tips; right view, both partially sectioned longitudinally; 15. Pericardial region and kidney, right view, right mantle wall partially removed; 16. Labial palps, ventral view, outer hemipalps deflected dorsally. Scale bars: 2 mm.

Figures 17 – 23. Cyrenoida implexa anatomical drawings. 17. Cerebral ganglia, ventral view; 18. Pedal ganglia, right view; 19. Visceral ganglia, ventral view; 20. digestive tubes and main musculature as in situ, right lateral view; 21. stomach, left lateral view; 22. Pericardial region, posterodorsal view, dorsal mantle wall partially removed; 23. stomach, right lateral view, right wall opened and deflected to show inner gastric surface. Scale bars: 2 mm.