Typosyllis antoni, Aguado & Helm & Weidhase & Bleidorn, 2015

Aguado, María Teresa, Helm, Conrad, Weidhase, Michael & Bleidorn, Christoph, 2015, Description of a new syllid species as a model for evolutionary research of reproduction and regeneration in annelids, Organisms Diversity & Evolution (New York, N. Y.) 15 (1), pp. 1-21 : 6

T. antoni n. sp.

Figures 1 , 2 , 3 , 4 , 5 , 6 , 7 , and 8

Material examined

Holotype MNCN 16.01 /15480, paratypes MNCN 16.01 / 15468-71, 16.01/15481 . Additional material UL. Leipzig aquarium, on algae and sandy bottom.

Comparative material examined

Figures

Fig. 1 Light microscopy pictures of Typosyllis antoni n. sp. a Live specimen, anterior end, dorsal view. b Detail of anterior end, dorsal view. c Detail of proventricle, dorsal view. Scales a 0.8 mm; b, c 0.3 mm

Fig. 2 SEM images of Typosyllis antoni n. sp. a Anterior end, dorsal view. b Anterior end, ventral view. c Detail of anterior end, dorsal view. d Anterior end, lateral view. e Midbody segments, ventral view. f Midbody parapodia, lateral view

Fig. 3 SEM images of Typosyllis antoni n. sp. a Chaetae fascicle, anterior parapodium. b, c Most dorsal chaetae, anterior parapodium. d, e Most dorsal chaetae, posterior parapodium. f Medially located chaetae, posterior parapodium. g Most ventral chaetae, posterior parapodium. h

Fig. 4 Light microscopy pictures of Typosyllis antoni n. sp. a Anterior chaetae, most dorsal ones and medially located in the fascicle. b Anterior chaetae, most dorsal ones. c Anterior chaetae, medially and most ventral ones. d. Anterior aciculae. e Midbody chaetae, most dorsal ones and medially located in the fascicle. f Midbody chaeta, most dorsal one. g Dorsal simple chaeta, posterior parapodium. h Ventral simple chaeta,

Fig. 5 Confocal maximum projections of Typosyllis antoni n. sp. Phalloidin–rhodamine labeling of adult specimens. Anterior is up in b and c, down in a and d, and right in e and f. a Lateral view of the anterior region, with everted pharynx (ph) and prominent dorsal cirri (dc), antennae (an), and palps (pl). The antennae exhibit prominent basal muscular sockets (dotted ring). b Ventral view of the anterior region showing the distinct longitudinal muscle bundles (lm) and the transverse muscle fibers (tm) forming semicircular fibers. c Dorsal view of the anterior region. Distinct longitudinal muscle fibers (lm) and the partly diagonal cross bracing transverse muscle fibers (tm) are exhibited. d Dorsal view of the posterior end showing the prominent pygidium (py) lacking distinct muscle bundles and the pygidial cirri (pc). e Lateral view of the midbody region, dorsal is up. The dorsal cirri (dc) insert at the base of the parapodium (pa). f Dorsolateral view of a midbody parapodial region. The dorsal cirri (dc) are represented by a prominent staining exhibiting a striped pattern; an antenna, ch chaetae, dc dorsal cirrus, lm longitudinal muscle fiber, mo mouth opening, pa parapodium, pc pygidial cirri, ph pharynx, pl palps, pp pharyngeal papillae, pr parapodial retractor muscle, py pygidium, tm transverse muscle fiber, vc ventral cirrus.Scale bar= 100 μm

Fig. 6 Confocal maximum projections of Typosyllis antoni n. sp. Phalloidin–rhodamine (gray) and serotonin labeling (red) of cross sections (a–c) and the proventricle (d, e) of adult specimens. Dorsal is up in a–c. Anterior is up in d and right in e. a Cross section of the midbody region, the prominent longitudinal muscle bundles are colored in yellow. The insert shows a detailed view of the parapodium, and major muscle bundles are color coded— parapodial retractor muscle in brown, acicular protractor muscle in green, acicular flexor muscle in violet, chaetal flexor muscle in pink, cirral muscle bundle in blue. b Cross section showing the distinct proventricle (pr) filling almost the whole body cavity. The longitudinal muscle bundles are color coded in yellow. c The proventricle (pr) and the ventral nerve cord (vn) show distinct serotonergic immunoreactivity. d The separated proventricle (pr) exhibits radial honeycomb-like muscle bundles (rb, dotted circle) and prominent circular muscle fibers (cf) surrounding the whole structure. e The separated proventricle (pr) is represented by an anterior circular muscle bundle (am) and suspending muscles (sm) terminating at the border between the anterior circular muscle bundle (am) and the radial muscle bundles (rb). am anterior circular muscle bundle, cf circular muscle fiber, dc dorsal cirrus, dv dorsoventral muscle fibers, in intestine, mm median muscle bundle, pa parapodium, pr proventricle, rb radial muscle bundle, sm suspending muscle fiber, vn ventral nerve cord. Scale bar=100 μm (color figure online)

Fig. 7 Typosyllis antoni n. sp. a Male stolon, dorsal view. b Female stolon, dorsal view. c Male stolon still attached to the parental body, dorsal view. d Female stolon still attached to the parental body, dorsal view. e Detail of anterior appendages of male stolon, dicerous kind, dorsal view. f Ovaries within posterior segments of parental body and female stolon still attached. g Posterior segments with ovaries of a female that has not developed a stolon yet