Leptodactylus watu

Da Silva, Leandro A., Magalhães, Felipe M., Thomassen, Hans, Leite, Felipe S. F., Garda, Adrian A., Brandão, Reuber A., Haddad, , 2020, Unraveling the species diversity and relationships in the Leptodactylus mystaceus complex (Anura: Leptodactylidae), with the description of three new Brazilian species, Zootaxa 4779 (2), pp. 151-189: 167-170

publication ID

https://doi.org/10.11646/zootaxa.4779.2.1

publication LSID

lsid:zoobank.org:pub:C5769EAA-CAF6-441F-A22F-D483E7E969C7

DOI

http://doi.org/10.5281/zenodo.3851227

persistent identifier

http://treatment.plazi.org/id/4F7E575F-CA2E-C200-359A-ADAEFAA8FE97

treatment provided by

Plazi

scientific name

Leptodactylus watu
status

sp. nov.

Leptodactylus watu  sp. nov.

( Figs. 2View FIGURE 2, 4a, 4cView FIGURE 4, 5hView FIGURE 5, 6hView FIGURE 6, 10View FIGURE 10) [http://zoobank.org/ urn:lsid:zoobank.org:act:5D79A081-C50C-463A-8101-04351E87AD23 ]

Leptodactylus aff. spixi: Guimarães et al. (2019) 

Holotype. UFMG 21332View Materials, an adult male from Parque Estadual do Rio Doce (19.753922°S, 42.633047°W, approximately 280 m a.s.l.), in Marliéria, Minas Gerais State, southeastern Brazil, collected by H. Thomassen on 21 November 2017.GoogleMaps 

Paratypes. Seven adult males ( UFMG 21327–21331View Materials and 21337–21338) and five adult females ( UFMG 21333– 21336View Materials and 21339) collected from the type locality by H. Thomassen from November 2017  to February 2018.

Additional material. One genetic voucher ( UFMG 16995) from Santa Bárbara do Leste, Minas Gerais State ( Fig. 1View FIGURE 1).

Etymology. The word watu  , a noun in apposition, is derived from the dialect of the Borún indigenous people and is a reference to the Doce River ( Costa-Reis & Genovez 2013). In 2015, the Doce River experienced the worst environmental disaster that ever took place in Brazil, the collapse of a mining tailings dam owned by Samarco (and co-owned by the Brazilian Vale and Australian BHP Billiton). The catastrophic dam failure released around 60 million cubic meters of iron ore tailings (toxic slurry) directly into the Doce River watershed, killing 20 people and affecting biodiversity across hundreds of kilometers around the river drainage, riparian lands, and the Atlantic coast, in addition to a severe contamination of the soil and water table within the affected region. The epithet is a tribute to the resistance of the Borún people and to the Watu (Doce River) in southeastern Brazil.

Diagnosis. Leptodactylus watu  is characterized by the following combination of character states: (1) small to moderate size, male SVL = 38.5–42.2 mm, female SVL = 40.5–47.9 mm; (2) sole of foot with few, non-obvious tubercles; (3) advertisement call composed of single, pulsed notes; (4) note duration varying from 27–70 ms; (5) note dominant frequency varying from 495–1098 Hz; (6) frequency upsweep varying from 0–560 Hz.

Comparisons with members of the L. mystaceus  complex. Leptodactylus watu  is distinguished from other species belonging to the L. mystaceus  complex, except L. barrioi  , L. kilombo  and L. notoaktites  , by having the sole of the foot covered with few, non-obvious tubercles, whereas L. didymus  , L. elenae  , L. mystaceus  , and L. spixi  have prominent tubercles ( Heyer 1978, 1983; Heyer et al. 1996). The new species can be further distinguished from some species by a smaller size ( Table 2): L. didymus  (male SVL = 45.9–52.2 mm, mean = 49.3; female SVL = 45.8–53.5 mm, mean = 49.8; Heyer et al. 1996), L. mystaceus  (male SVL = 42.4–52.2 mm, mean = 47.4; female SVL = 44.8– 56.1 mm, mean = 50.0; Heyer et al. 1996), and L. notoaktites  (male SVL = 42.5–54.2 mm, mean = 47.5; female SVL = 43.4–55.8 mm, mean = 48.0; de Sá et al. 2014). Members of the L. mystaceus  complex are mainly distinguished from each other by their distinct advertisement calls ( Fig. 6View FIGURE 6).

Acoustic comparisons in the L. fuscus  group. The advertisement call of Leptodactylus watu  is composed of single, pulsed notes ( Table 3; Fig. 6hView FIGURE 6), differing from species with trill calls ( L. cunicularius  , L. cupreus  , L. oreomantis  , and L. plaumanni  ; Fig. 7View FIGURE 7; Carvalho et al. 2013) and species with nonpulsed calls ( L. albilabris  , L. bufonius  , L. camaquara  , L. didymus  , L. elenae  , L. furnarius  , L. fuscus  , L. gracilis  , L. jolyi  , L. kilombo  sp. nov., L. laticeps  , L. latinasus  , L. longirostris  , L. marambaiae  , L. mystacinus  , L. notoaktites  , L. poecilochilus  , L. sertanejo  , L. spixi  , L. syphax  , L. tapiti  , and L. troglodytes  ). From other species with pulsed calls, L. watu  is distinguished by having its call formed by partly fused pulses, whereas the calls of L. barrioi  , L. mystaceus  and L. cf. mystaceus  are formed by complete pulses ( Fig. 6View FIGURE 6). From species also having calls with partly fused pulses ( L. caatingae  , L. fragilis  , and L. labrosus  ), L. watu  is distinguished by a shorter note (27–70 ms) from L. fragilis  (> 100 ms; Heyer et al. 2006). The calls of L. watu  , L. caatingae  , and L. labrosus  are the most similar to each other in the L. fuscus  group, but these species can be distinguished, although value ranges partially overlap, by differences in the dominant frequency ( L. watu  : 495–1098 Hz, mean = 763; L. caatingae  : 1148–1430 Hz, mean = 1315, in our sample; 943–1620 Hz, mean = 1423 Hz, in the original description; Heyer & Juncá 2003) or duration ( L. watu  : 27–70 ms, mean = 40; L. labrosus  : 64–133 ms, mean = 100; Carvalho & Ron 2011) of their calls. Still, these species differ markedly in morphology and size (see previous paragraph; Heyer 1978; Heyer & Juncá 2003).

Description of holotype. Body robust. Snout sub-elliptical in dorsal and ventral views ( Fig. 10View FIGURE 10 a–b), acuminate in lateral view ( Fig. 10eView FIGURE 10). Canthus rostralis rounded; loreal region flat; tympanic annulus well-defined, circular (TD = 62.4% ED); supratympanic fold from the posterior corner of the eye, passing over the dorsal edge of the tympanic annulus, and ending at the base of the arm; vocal sac subgular; vocal slits present; vomerine teeth in two nearly straight rows, almost connected, medial and posterior to choanae and almost parallel to sagittal plane. Tongue rounded, free at its posterior third. Relative finger lengths II = IV <I <III; fingers without lateral fringing or webbing; finger tips rounded, unexpanded; inner metacarpal tubercle oval, divided, outer metacarpal tubercle rounded; outer metacarpal tubercle twice the maximum width of the inner; subarticular tubercles rounded; supernumerary tubercles absent ( Fig. 9cView FIGURE 9). Dorsal surfaces of body and forelimbs smooth, shagreened on flank and dorsal surface of shank. Dorsolateral fold from the posterior corner of the eye, extending posteriorly to the groin. Flank with a fragmented line of granules posteriorly. Dorsal surface of hindlimbs with tiny tubercles (more conspicuous in life). Ventral surface of body and limbs smooth, underside of thigh areolate. Relative toe lengths I <II <V <III <IV; toes without lateral fringing or webbing; toe tips unexpanded. Inner metatarsal tubercle elongated, twice the maximum length of the ovoid outer metatarsal tubercle ( Fig. 10dView FIGURE 10). Tarsal fold extending 2/3 of tarsus length, from the inner metatarsal tubercle towards the heel; subarticular tubercles rounded; sole of foot with tiny tubercles, inconspicuous.

Colors. In life, dorsal surfaces (body and limbs) light brown with dark gray blotches poorly delimited ( Figs. 4a, cView FIGURE 4, 5hView FIGURE 5). Upper region of the loreal region black, lower region light cream, lips dark brown. Black stripe on the supratympanic fold, delimited dorsally by a white line. Light cream stripe on the dorsolateral fold, more conspicuous posteriorly. Flank light gray. Tympanic membrane dark brown on the center and upper rim, light brown laterally and on ventral rim. Throat immaculate white with nonpigmented portions near the jaw. Ventral surface of arms, legs (except tarsus), and belly nonpigmented; tarsus with scattered dark brown stains. Weakly defined dark brown transversal bars on the upper surface of hindlimbs and forearm. Posterior surface of thigh with a yellow longitudinal stripe bordered by two black stains; upper portion of with black spots and stains. Ventral surface of hand, foot, and tarsus dark brown interspersed with nonpigmented areas. In preservative, dorsal surfaces (body and hindlimbs) light gray with dark gray blotches poorly delimited; forearms light cream with scattered dark gray dots and spots ( Fig. 10View FIGURE 10). Upper portion of the loreal region black, lower half cream-colored, lips blackish gray. Black stripe on the supratympanic fold. White stripe on the dorsolateral fold, more conspicuous posteriorly. Flank light gray. Tympanic membrane light brown with the center and upper edge dark gray. Ventral surfaces cream-colored. Throat with few gray dots and stains near the jaw laterally. Ventral surface of hindlimbs with few dark gray spots. Dark brown transversal bars on the dorsal surface of hindlimbs and forearm. Posterior surface of thigh with a white longitudinal stripe bordered by two black stains. Ventral surface of hand, foot, and tarsus dark brown interspersed with nonpigmented areas.

Intraspecific variation. In life, dorsal coloration varies from brown, sometimes conspicuous reddish shades, or dark gray. The dorsal light brown coloration in life becomes light gray in preservative. Dark brown blotches on the dorsum can be present, more conspicuous in preserved specimens. Dark brown transversal bars on the upper surface of thigh and tibia are more evident in preserved species. Lateral line of tubercles is poorly evident in most specimens. Inner metacarpal tubercle is rounded in UFMG 21329.

Advertisement call. Description based on 129 calls recorded from two males ( Table 3). The call ( Fig. 6hView FIGURE 6) consists of single, pulsed notes given at a rate of 124–275 per minute. Note duration varies from 27–70 ms. Rise time varies from 37–80% of note duration. Notes are formed by 2–7 partly fused pulses emitted at a rate of 57–153 per second. The dominant frequency coincides with the fundamental harmonic at 495–1098 Hz. Notes have no frequency modulation or a modest frequency upsweep throughout their duration, varying from 0–560 Hz.

Habitat and natural history. At the type locality, Leptodactylus watu  was found calling during the rainy season in the region from late October to late February. Calling is strongly associated with rainy days or few days after heavy pours. Males were found calling around flooded areas or close to moist ground in both open areas (transitional marshlands between the forest and lakes) and inside the forest. All calling males were hidden either under dense vegetation, ground leaf litter or inside underground chambers. They were never found calling in pastures. The congeneric species found calling in syntopy with L. watu  were L. fuscus  , L. latrans  , and L. labyrinthicus  .

Distribution. Leptodactylus watu  is only known from two localities in a region characterized by lowland interior Atlantic forests associated with the upper-middle Doce River drainage, in the Brazilian state of Minas Gerais ( Fig. 1View FIGURE 1): (1) the type locality, at Parque Estadual do Rio Doce, and (2) Santa Bárbara do Leste, approximately 70 km southeast from the type locality.

Remarks. The identity of populations assigned to Leptodactylus aff. mystaceus ( Santana et al. 2010)  , L. aff. spixi ( Ferreira et al. 2019)  , and L. spixi  ( Rödder et al. 2007; Silva-Soares & Scherrer 2013; Pereira-Ribeiro et al. 2019) from different localities in southeastern and northeastern Brazil should be addressed using calls and/or DNA sequences, as these records could correspond to L. barrioi  or L. watu  , or additional candidate new species.

UFMG

Universidade Federal de Minas Gerais

Kingdom

Animalia

Phylum

Chordata

Class

Amphibia

Order

Anura

Family

Leptodactylidae

Genus

Leptodactylus

Loc

Leptodactylus watu

Da Silva, Leandro A., Magalhães, Felipe M., Thomassen, Hans, Leite, Felipe S. F., Garda, Adrian A., Brandão, Reuber A., Haddad, 2020
2020
Loc

Leptodactylus aff. spixi: Guimarães et al. (2019)

Guimaraes 2019
2019