Ganaspis kimorum Buffington, 2024

Sosa-Calvo, Jeffrey, Forshage, Mattias & Buffington, Matthew L., 2024, Circumscription of the Ganaspis brasiliensis (Ihering, 1905) species complex (Hymenoptera, Figitidae), and the description of two new species parasitizing the spotted wing drosophila, Drosophila suzukii Matsumura, 1931 (Diptera, Drosophilidae), Journal of Hymenoptera Research 97, pp. 441-470 : 441-470

Ganaspis kimorum Buffington sp. nov.

Figs 1 C , 2 , 3 E, F , 4 B, D, E

Material examined.

Holotype. Japan: Tokyo Kimura Lab, field collected G. xanthopoda ‘ suzukii type’ Collected June 2010 Received USNM July 2015; female, USNMENT 00877810 . Deposited in USNM . Paratypes: Japan: Tokyo Kimura Lab, field collected G. xanthopoda ‘ suzukii type’ Collected June 2010 Received USNM July 2015; USNMENT 00877811 , USNMENT 00877772 , USNMENT 00877823 ; Kanto Province, Tokyo district, Hachioji, Naganuma Park 35 ° 38 ' 12 " N, 139 ° 21 ' 54 " E Ex larva of Drosophila suzukii Coll. 3. VI. 2015, Leg. N. Ris & P. Girod; USNMENT 01025537 – USNMENT 01025541 ; Tokyo, Hachioji Naganuma Park 35.6368 ° N, 139.3647 ° E, ex D. suzukii on Prunus serrulata 16 Jun 2017; Girod, collector CABI colony voucher G 1 _ Tokyo; USNMENT 01867459 , USNMENT 01867458 , USNMENT 01025536 . China: Yunnan Province, Kunming district, Kunming , Yunnan Agricultural University , 25 ° 07 ' 41 " N, 102 ° 44 ' 50 " E, Ex larva of Drosophila suzukii Coll. 3. VI. 2015, Leg. M. Kenis; USNMENT 01025542 – USNMENT 01025546 ; Yunnan Province, Honghe district, Shiping 23 ° 41 ' 15 " N, 102 ° 32 ' 53 " E Ex larva of Drosophila suzukii Coll. 6. VI. 2015, Leg. M. Kenis; USNMENT 01025547 – USNMENT 01025551 ; Yunnan Province, Kunming district, Kunming , Yunnan Agricultural University 25 ° 07 ' 41 " N, 102 ° 44 ' 50 " E Ex larva of Drosophila suzukii Coll. 3. VI. 2015, Leg. M. Kenis; USNMENT 01025552 , USNMENT 01025553 ; USNMENT 01734709 ; Yunnan, Kunming Xining Temple 25.1072 ° N, 102.7167 ° E, ex D. suzukii / D. pulchrella ; on Prunus sp. ; 17 May 2017 Kenis, collector CABI colony voucher G 1 _ Yunnan; USNMENT 01867468 , USNMENT 01867467 , USNMENT 01867454 – USNMENT 01867457 , USNMENT 01025528 . Yunnan Province, Honghe district, Shiping 23 ° 41 ' 15 " N, 102 ° 32 ' 53 " E Ex larva of Drosophila suzukii Coll. 6. VI. 2015, Leg. M. Kenis; USNMENT 01734706 , USNMENT 01734705 , USNMENT 01025530 , USNMENT 01734714 . Yunnan, Kunming, Dong Da Cun , Pan Long District , 25.098602 ° N 102.835000 ° E, 2239 m, ex D. suzukii on Sambucus adnata ; collected 25. VII. 2016, emerged 24. VIII. 2016 Giorgino and Guerreiri DSZ 118 , DSZ 137 , DSZ 188 ; USNMENT 01025531 – USNMENT 01025533 ; Yunnan, Dali, ex D. suzukii / Sambucus williamsii ; 16 Jun 2017 Kenis, collector CABI colony voucher G 1 _ Dali; USNMENT 01867465 , USNMENT 01867466 . Paratypes deposited in USNM .

Diagnosis.

Separated from G. brasiliensis by the completely smooth lateral aspect of the scutellum (Fig. 3 E ). Separated from G. lupini by the less-developed ovipositor clip that does not extend past the midwidth of the ovipositor valve (Fig. 4 B, E ). In G. lupini the ovipositor clip expands across more than half the width of the ovipositor valve (Fig. 4 C ).

Description.

As in description for G. brasiliensis species complex, but with the lateral aspect of scutellum completely smooth; ovipositor clip reduced, not extending beyond the halfway point across the fused ovipositor valve. Previous studies referencing ‘ Gb G 1 ’ or ‘ G 1 ’ refer to this species ( Nomano et al. 2017; Giorgini et al. 2018; Abram et al. 2022).

Etymology.

Named in honor of Prof. Kimura (Hokkaido University, retired) and Dr Kim Hoelmer ( USDA-ARS, retired). The name is a combination of Kimura and Kim Hoelmer.

Cytochrome c oxidase subunit I ( COI) Barcode region.

> Ganaspis _ kimorum _ BBP 857

NATATTATATTTTATTTTTGGTATTTGATCAGGAATAATTGGATCAAGTTTAAGAATAATTATTCGATTA GAATTAGGAACCCCTTCACAATTAATTAATAATGATCAAATTTATAATACAATTGTTACTACTCATGC ATTTGTAATAATTTTTTTTATAGTTATACCAATTATAGTTGGAGGATTTGGAAATTACTTAATTCCTT TAATATTATCTGCTCCTGATATATCATTCCCTCGTCTTAATAATATAAGATTTTGATTATTAATCCCT TCTTTAATTTTAATAATTTCAAGTATATTTATTGATGAAGGGTCTGGAACTGGATGAACAGTTTATCC TCCTTTATCACTAAATAAGTCCCACCCAGGAATCTCAACTGACTTAGTAATTTTTTCTCTTCATCTTA GAGGAATTTCTTCAATTTTAGGATCAATTAATTTTATTACAACTATTCTAAATATACGACCAAATTTA ATAAGTATAGATAAAATTTCTTTATTTACTTGATCCATTTTTCTTACCACTATTTTATTATTATTATC TTTACCAGTATTAGCAGGTGGAATCACTATATTACTTTTTGACCGAAATATTAATACATCTTTTTATG ACCCAATAGGAGGAGGAGACCCAATTCTATACCAACACTTATTT

> Ganaspis _ kimorum _ 233137974 _ E 02 (partial sequence, 592 bp)

ATTAGAATTAGGAACCCCTTCACAATTAATTAATAATGATCAAATTTATAATACAATTGTTACTACTCAT GCATTTGTAATAATTTTTTTTATAGTTATACCAATTATAGTTGGAGGATTTGGAAATTACTTAATTCC TTTAATATTATCTGCTCCTGATATATCATTCCCTCGTCTTAATAATATAAGATTTTGATTATTAATCC CTTCTTTAATTTTAATAATTTCAAGTATATTTATTGATGAAGGGTCTGGAACTGGATGAACAGTTTAT CCTCCTTTATCACTAAATAAGTCCCACCCAGGAATCTCAACTGACTTAGTAATTTTTTCTCTTCATCT TAGAGGAATTTCTTCAATTTTAGGATCAATTAATTTTATTACAACTATTCTAAATATACGACCAAATT TAATAAGTATAGATAAAATTTCTTTATTTACTTGATCCATTTTTCTTACCACTATTTTATTATTATTA TCTTTACCAGTATTAGCAGGTGGAATCACTATATTACTTTTTGACCGAAATATTAATACATCTTTTTA TGACCCAATAGGAGGAGGAGACCCAATTCTATACCAACACTTATTT

> Ganaspis _ kimorum _ 233137957 _ F 06

NATATTATATTTTATTTTTGGTATTTGATCAGGAATAATTGGATCAAGTTTAAGAATAATTATTCGATTA GAATTAGGAACCCCTTCACAATTAATTAATAATGATCAAATTTATAATACAATTGTTACTACTCATGC ATTTGTAATAATTTTTTTTATAGTTATACCAATTATAGTTGGAGGATTTGGACATTACTTAATTCCTT TAATATTATCTGCTCCTGATATATCATTCCCTCGTCTTAATAATATAAGATTTTGATTATTAATCCCT TCTTTAATTTTAACAATTTCAAGTATATTTATTGATGAAGGATCTGGAACCGGATGAACAGTTTATCC TCCTTTATCACTAAATAAGTCCCACCCAGGAATCTCAACTGACTTAGTAATTTTTTCTCTTCATCTTA GAGGAATTTCTTCAATTTTAGGATCAATTAATTTTATTACAACTATTCTAAATATACGACCAAATTTA ATAAGTATAGATAAAATTTCTTTATTTACTTGATCCATTTTTCTTACCACTATTTTATTATTATTATC TTTACCAGTATTAGCAGGTGGAATCACTATATTACTTTTTGACCGAAATATTAATACATCTTTTTATG ACCCAATAGGAGGAGGAGACCCAATTCTATACCAACACTTATTT

Biology.

Koinobiont endoparasitoid of Drosophila suzukii ( Nomano et al. 2017; Girod et al. 2018 b; Wang et al. 2018; Seehausen et al. 2020; Daane et al. 2021). Seehausen et al. (2020) found ‘ Ganaspis cf. brasiliensis ’ G 1 could successfully parasitize D. melanogaster in lab culture.

Figures

Figure 1. Type-specimens of the species belonging to the G. brasiliensis species complex A brasiliensis, lectotype B kimorum, holotype C lupini, holotype.

Figure 2. External anatomy of Ganaspis kimorum, a member of the Ganaspis brasiliensis species complex A head and mesosoma, lateral view B head and mesosoma, dorsal view C femal antennae D metapectal-propodeal complex, lateral view E fore- and hindwings, female F propodeum, dorso-lateral view.

Figure 3. Comparison of the scutellar morphology between G. brasiliensis (A, B), G. lupini (C, D) and G. kimorum (E, F). Black arrows indicate sculptured (A) and smooth (C, E) lateral aspect of scutellum.

Figure 4. Comparison of the ovipositor clip between G. lupini (A, C) and G. kimorum (B, D). A G. lupini ovipositor tip, lateral view B G. kimorum, ovipositor tip, lateral view C G. lupini ventral of ovipositor showing ovipositor clip and membrane D G. kimorum ventral of ovipositor showing ovipositor clip and membrane. Black arrows indicate the location of the ovipositor clip.