Tuber songlu S.P. Wan, 2021

Wan, Shanping, Liu, Jianwei, Huang, Lanlan, Qin, Xiaomin, Liu, Wei & Yu, Fuqiang, 2021, Tuber qujingense and T. songlu, two new species from Yunnan, China, Phytotaxa 527 (4), pp. 248-256 : 253-254

Tuber songlu S.P. Wan , sp. nov. ( Fig. 4 )

MycoBank: MB 839734

Typification: CHINA. Yunnan Province, Huize County (29.103°E, 25.56°N), in humic soil under a pure Pinus armandii forest, at about 2415 m, 14 August 2016, wsp695, HKAS 95771 (GenBank Acc. No.: ITS = KX904883 , LSU = KY 013656 ).

Diagnosis: Tuber songlu differs from other species by its whitish ascomata, dense spine-like dermatocystidia, ellipsoid ascospores and 1–4 spored asci.

Etymology: Refers to the local name of the fungus.

Description: Ascomata 1–2 cm in diam, globose to subglobose or irregular, white, light reddish brown when fresh, becoming brown when dried. Peridium 130–500 µm thick, densely pubescent, one layer, prosenchymatous, composed of subglobose to subangular cells, (1–) 1.5–18(–23) × (1–) 1.5–7(–8.5) µm, hyaline. Gleba solid, brown when mature, marbled with brownish yellow veins, composed of hyaline, interwoven, thin-walled cylindrical hyphae, 1.5–8.5 µm broad at the septa, and interwoven, inflated hyphae 2–8.5 µm broad. Dermatocystidia, straight or bent, obtuse or apiculate at the tip, up to 230 µm long, 7 µm in diam, multiseptate, hyaline to whitish. Asci (63–) 72–96 × 54–75 (–81) µm, globose to subglobose, pyriform, ellipsoid or irregular, hyaline, sessile or with a short stalk, thin-walled 1–2 µm thick, 1–4 spored. Ascospores, ellipsoid, sometimes broadly ellipsoid, subglobose, hyaline when young, becoming brown at maturity; excluding their alveolate-reticulate ornamentation, in 1-spored asci (41–) 42.5–51.5 (–56) × (26.5–) 32.5–40 (–41) µm (Q = 1.10–1.70), in 2-spored (22–) 24–42 (–46) × (18–) 19–34 µm (Q = 1.10–1.51), in 3-spored (20–) 26–33 (–35) × 19 (–21)–29 (–30) µm (Q = 1.13–1.24), and in 4-spored (19–) 20–27.5 (–29) × 15–17 (–27) µm (Q = 1.1–1.2); reticulum with 3–8 meshes along the spore length and 3–8 across. The alveolar walls up to 1–7 µm tall.

Distribution and habitat: Hypogeous, in soil under a pure forest of Pinus armandii in Yunnan Province, China. Known only from China.

Other material examined: CHINA. Yunnan Province, Province , Huize County, in soil under a pure forest of P. armandii , December 2016, wsp701, HKAS 95777 (GenBank Acc. No.: ITS = KX904884 , LSU = KY 013657 ) ; wsp749, HKAS 95851 (GenBank Acc. No.: ITS = KX904886 , LSU = KY 013658 ) .

Figures

FIGURE 4. Tuber songlu (HKAS 95771, type) A. Ascomata and glebae; B–C. Dermatocystidia; D. Peridium section; E, F. Light micrograph (LM) of ascospores. Photos by: Shanping Wan.