Eodriloides majungianus ( Michaelsen, 1897 ) Hong, Yong, Razafindrakoto, Malalatiana, Blanchart, Eric, Szederjesi, Tímea & Csuzdi, Csaba, 2019

Hong, Yong, Razafindrakoto, Malalatiana, Blanchart, Eric, Szederjesi, Tímea & Csuzdi, Csaba, 2019, New Acanthodrilid Species From Madagascar (Clitellata, Acanthodrilidae), Acta Zoologica Academiae Scientiarum Hungaricae 65 (3), pp. 215-233 : 215-233

Eodriloides majungianus ( Michaelsen, 1897) comb. n.

Acanthodrilus majungianus Michaelsen, 1897: 218 .

Notiodrilus majungianus: Michaelsen 1900: 136 .

Eodrilus majungianus: Michaelsen 1907: 141 , Pickford 1937: 587.

Material eXamined. – HNHMAF/5688 1 eX. Ambodimanga, Ankarafantsika District Madagascar, 16°19’27.91”S, 46°49’52.90”E, 83 m a.s.l., miXed forest, soil and litter. Leg 04.03.2017, Yong Hong & Malalatiana Razafindrakoto ( MD19 ) .

Diagnosis – Clitellum on 13–18, Prostate Pores 17, 19 connected with al- most straight genital grooves just outside setal line b. SPermathecae elongated oval with two diverticles on the oPPosite sides of the duct ( Fig. 1). The Penial

218 HONG, Y., RAZAFINDRAKOTO, M., BLANCHART, E., SZEDERJESI, T. & CSUZDI, CS.

setae slightly sPatulate, 0.57 mm long and 0.013 mm wide, ornamented w scattered small serrations ( Fig. 2)

Remarks – Our sPecimen fits well to the original descriPtion of CHAELSEN (1897). The only notable difference is in the length of the clitellum which is stated on 13–17 by Michaelsen, but remarked that the hind end of clitellum is not fully develoPed yet.

Eodriloides metandricus Csuzdi, Razafindrakoto et Hong sP. n.

( Figs 3–6)

Material eXamined − HolotyPe: HNHM AF/5689 clitellate adult, Tsararano , Maro District, Madagascar, 16°10’55.99”S, 46°39’44.95”E, 48 m a.s.l. miXed forest, soil and 03.03.2017, Leg. Yong Hong & Malalatiana Razafindrakoto. ParatyPes : HNHM AF/ 3 clitellate adult + 13 aclitellate adult eX., HNHM AF/5691 2 clitellate adult + 9 aclitellate adult eX. Locality and date same as of HolotyPe .

Etymology − The sPecific ePithet refers to the metandric condition of the male Paratus.

Diagnosis − Length 86–96 mm, diameter 3–3.5 mm. Colour Pale, Pigmen tation lacking. First dorsal Pore in 7/8. Clitellum saddle- shaPed on 13–19. f Paired, Presetal outside b. Prostatic Pores 17, 19. SPermathecal Pores large 7/8, 8/9, sPermathecae with elongated oval amPoule, and an equally long bearing a Pair of acinous diverticula at the basis of the duct. Gizzard large

5, last Pair of hearts in 13. Calciferous glands lacking, vascular oesoPhagus 9–12. EXcretory system holoic avesiculate. Functionally metandric, vesicles 12. Penial setae Present, length 1 mm, diameter 0.035 mm. TiP slightly ornamentation dense transversal serrations.

DescriPtion − Length of the holotyPe 95 mm, width (Postclitellar) 3.5 mm. Segments No. 180, the last 15 segments regenerated. ParatyPes 86–96 mm in length and 3 mm in ameter, segment No. 188–192. Colour greyish, Pigmentation absent. Prostomium slightl ePilobic, dorsal Pores from 7/8. Segments simPle, sometimes with slight secondary

NEW ACANTHODRILID SPECIES FROM MADAGASCAR ( CLITELLATA) 219

sPermatheca

220 HONG, Y., RAZAFINDRAKOTO, M., BLANCHART, E., SZEDERJESI, T. & CSUZDI, CS.

nulation. Setae visible from segment 2, eight Per segment in closely Paired regular ro Setal formula after clitellum aa: ab: bc: cd: dd = 6.5:1.25:5.5:1:35 ( Fig. 3). Setae of 17, 19

fied to Penial setae, genital setae lacking. SPermathecal Pores Paired Prominent slits in intersegmental furrow 7/8, 8/9 lateral to seta b. Clitellum saddle- shaPed on 13–19. F Pores in 14, Presetal and slightly lateral to b. Two Pairs of Prostatic Pores in 17 and 19 just the base of seta b, joined by almost straight seminal grooves, running outside b. Male P minute, eXternally not visible on 18, within the seminal grooves. Genital markings Paired as three or four mid- ventral, intrasegmental PaPillae located in 7, 13, 14 and P

in 16 around the ventral setae ( Fig. 4).

First sePtum visible 5/6, sePta 6/7–11/12 thickened. One large oesoPhageal gizzard 5. Dorsal vessel single throughout, last Pair of hearts in 13. EXcretory system holoic, siculate. Calciferous glands missing but the oesoPhagus in 9–12 highly vascular. I begins in 16, tyPhlosole lacking. Metandric. One Pair of functional testis and iridescent male funnel in 11, accomPanied by a Pair of vestigial, not iridescent male funnel in Seminal vesicles a single Pair in 12. One Pair of moderate sized ovaries in 13. Two Pairs tubular Prostates of similar size in 17 and 19, somewhat coiled and confined to their segment. Penial setae small, ca. 1 mm in length and 0.035 mm in diameter. The tiP slightl bent, ornamentation dense transversal serrations ( Fig. 5). Two Pairs of sPermathecae and 9. AmPulla slightly elongate, oval, duct almost as long as the amPoule. A Pair of facing, acinous diverticulum joins to the duct just above the sPermathecal Pore ( Fig. 6

Remarks − The new sPecies is close to Eodriloides voeltzkowi ( Michaelsen 1907) comb. nov. but differs from it in its smaller dimensions (96 vs. 120 mm the metandric condition of the male aPParatus (vesicles in 12 vs. 9, 12) and in intersegmental oPenings of sPermathecae (vs. segmental on the rim of 8 and

Howascolex proprioporus Csuzdi, Razafindrakoto et Hong sP. n.

( Figs 7–10)

Material eXamined − HolotyPe: HNHM AF/5692 clitellate adult, Tsararano, Maro District, Madagascar, 16°10’55.99”S, 46°39’44.95”E, 48 m a.s.l., miXed forest, soil and 03.03.2017, Leg. Yong Hong & Malalatiana Razafindrakoto. ParatyPes: HNHM AF /

6 clitellate adult eX., HNHM AF/5694 1 clitellate adult eX. Locality and date same HolotyPe. HNHM AF/5696 4 clitellate adult eX., Ankazomborona, Marovay District, M gascar, 16°06’35.73”S, 46°46’58.13”E, 48 m a.s.l., miXed forest, soil and litter. 03.03.

Leg. Yong Hong & Malalatiana Razafindrakoto. HNHM AF/5697 2 clitellate adult eX., bodimanga, Ankarafantsika District , Madagascar, 16°19’27.91”S, 46°49’52.90”E, 83 m a miXed forest, soil and litter. 04.03.2017, Leg. Yong Hong & Malalatiana Razafindrakoto Etymology − The sPecific ePithet refers to the closely Paired genital Pores .

Diagnosis − Length 55–77 mm, diameter 3–4.5 mm. Colour Pale, mentation lacking. First dorsal Pore in 12/13. Clitellum circular on 13–18 14 closely Paired midventral. Prostatic Pores closely Paired on 17, 19. SP mathecal Pores closely Paired, segmental on small midventral PaPillae on 9. SPermathecae with an oval amPoule, and a half as long duct bearing a multilocular diverticulum at the basis of the duct. Gizzard large in 5?, last

NEW ACANTHODRILID SPECIES FROM MADAGASCAR ( CLITELLATA) 221

of hearts in 13. Calciferous glands large, eXtramural in 15. EXcretory system meroic with ventral megameronePhridia. Functionally metandric, vesicles in 12. Penial setae Present, length 0.7 mm, diameter 0.02 mm. TiP Pointed, slight- ly bent, ornamentation scattered teeth.

DescriPtion − Length of the holotyPe 77 mm, width (Postclitellar) 4.5 mm. Segments

No. 191. ParatyPes 55–68 mm in length and 3–4 mm in diameter, segment No. 159–184 10 = sPermatheca

222 HONG, Y., RAZAFINDRAKOTO, M., BLANCHART, E., SZEDERJESI, T. & CSUZDI, CS.

Colour greyish, Pigmentation absent. Prostomium schizolobic, dorsal Pores from 12 Segments before clitellum clearly triannulate, after clitellum not so eXPressed. Setae v from segment 2, eight Per segment in moderately Paired regular rows. Setal formula clitellum aa: ab: bc: cd: dd = 3.1:1:2.8:1.8:25 ( Fig. 7). Setae of 17, 19 modified to Penial Genital setae lacking. SPermathecal Pores segmental, closely Paired on midventral PaP in segments 8 and 9. Clitellum circular on 13–18. Female Pores closely Paired, midv on 14. Two Pairs of Prostatic Pores in 17 and 19 ventral to setae a, joined by angle- q shaPed seminal grooves, running between a–a. Male Pores minute, eXternally not v

on 18, within the seminal grooves. Paired genital markings Present in 17, 18, 19 ( Fig. 8 First sePtum visible 8/9, sePta 9/10–12/13 slightly thickened. One large oesoP gizzard in front of the first sePtum (Possibly in 5). Dorsal vessel single throughout, Pair of hearts in 13. EXcretory system meroic throughout with ca. 14 minute, biramous meronePhridia and one ventral row of larger meronePhridia (megameronePhridia?) side. Calciferous glands large, eXtramural in 15. Intestine begins in 17, tyPhlosole bilobate. Metandric. One Pair of functional testis and iridescent male funnel in 11. There a vestigial, not iridescent male funnel in 10 and a single Pair of seminal vesicles in 12.

Pair of ovaries in 13. Two Pairs of tubular Prostates of similar size in 17 and 19, somew coiled and confined to their own segment. Penial setae small, ca. 0.7 mm in length and mm in diameter. The tiP Pointed, slightly bent, ornamentation scattered small teeth (

9). Two Pairs of sPermathecae in 8 and 9. AmPulla ovoid, duct ca. half as long as the Poule. A stalked diverticulum containing 3–4 sPerm- balls joins to the duct just above sPermathecal Pore ( Fig. 10).

Remarks − This sPecies is unique in Howascolex by its medially closened genital Pores.

Howascolex vohimanus Razafindrakoto, Blanchart et Csuzdi sP. n.

( Figs 11–15)

Material eXamined − HolotyPe: HNHM AF/5698 clitellate adult, Vohimana Reser Madagascar, 18°55’13.0800”S, 48°31’03.36”E, rainforest. Leg. Malalatiana Razafindrakoto Eric Blanchart, 17.04.2008. ParatyPes: HNHM AF/5699 1 clitellate adult + 1 aclitellate eX. Locality and date same as of holotyPe .

Etymology – The sPecific ePithets refers to the tyPe locality, Vohimana Nature serve.

Diagnosis − Length 80–102 mm, diameter 4 mm. Colour Pale, Pigmenta tion lacking. First dorsal Pore in 11/12. Clitellum saddle- shaPed on 13–18. f Presetal in b. Prostatic Pores Paired on 17, 19. SPermathecal Pores segmental 8, 9 between a–b. SPermathecae with an elongated oval amPoule, and a as long duct bearing several small intramural sPerm chambers. Free diverticu lum lacking. Gizzard large in 5, last Pair of hearts in 13. Calciferous large, intramural in 15. EXcretory system meroic with large ventral megamero nePhridia. Holandric, vesicles in 9, 12. Genital setae Present, length ca. 1.3 diameter 0.025 mm, ornamentation small scales. Penial setae slightly undu lated, length ca. 4 mm, diameter 0.01 mm, ornamentation lacking.

NEW ACANTHODRILID SPECIES FROM MADAGASCAR ( CLITELLATA) 223

whole seta, B = tiP of the seta. 15 = sPermatheca

224 HONG, Y., RAZAFINDRAKOTO, M., BLANCHART, E., SZEDERJESI, T. & CSUZDI, CS.

DescriPtion – Length of the holotyPe (Posterior amPutee) 80 mm, width (Postclitel lar) 4 mm. Segments No. 123. Adult ParatyPes 102 mm in length and 4 mm in diameter segment No. 160. Colour greyish, Pigmentation absent. Prostomium schizolobic, Pores from 11/12. Segments before clitellum clearly triannulate, after clitellum not so Pressed. Setae visible from segment 2, eight Per segment in moderately Paired rows. Setal formula after clitellum aa: ab: bc: cd: dd = 5.6:1.2:5.6:1:20 ( Fig. 11). Setae of 17 modified to Penial setae, of 8, 9 modified to genital setae. SPermathecal Pores segmental on 8, 9 between setae a–b. Clitellum saddle- shaPed on 13–18. Female Pores on 14 P in line with setae b. Two Pairs of Prostatic Pores in 17 and 19 somewhat ventral to setae joined by almost straight seminal grooves. Male Pores eXternally not visible on 18, w the seminal grooves. Genital PaPillae around ab of 8 and 9, scattered genital markings 16, 20 and inside the genital field ( Fig. 12).

First sePtum visible 5/6, sePta 8/9–11/12 thickened. One large oesoPhageal gizzard 5. Dorsal vessel single throughout, last Pair of hearts in 13. EXcretory system with a of large ventral megameronePhridia and several small, hardly recognizable meronefridia scattered on the body wall. Calciferous glands large, intramural in 15. Intestine begins 18, tyPhlosole small, lamellar. Holandric. Two Pairs of testes and iridescent male in 10, 11 and vesicles in 9, 12. One Pair of ovaries in 13. Two Pairs of tubular Prostates similar size in 17 and 19, large but confined to their own segment. Penial setae long, and slightly undulated, ca. 4 mm in length and 0.01 mm in diameter. Ornamentation ing ( Fig. 13). Genital setae ca. 1.3 mm in length and 0.025 mm in diameter, the ornamenta tion consist of scattered denticulate scales ( Fig. 14). Two Pairs of sPermathecae in 8 9. AmPulla elongated sac- shaPed, duct ca. 1/3 as long as the amPoule containing sev intramural sPerm chambers. Free sPermathecal diverticulum lacking ( Fig. 15).

Remarks − The new sPecies is similar to H. hyalochaeta (Michaelsen, comb. nov., but differs from it in the segmental sPermathecal Pores and much longer Penial setae. In the segmental sPermathecal Pores and the P ence of genital setae H. vohimana sP. n. is similar to H. farafangana C Razafindrakoto et Hong, 2016 as well, but clearly differs from it in the ture of the sPermathecae.

Acanthodrilus hesperus Razafindrakoto, Blanchart et Csuzdi sP. n.

( Figs 16–20)

Material eXamined − HolotyPe. HNHM AF/5673 clitellate adult. Morondava, M gascar, 20°17’40.9”S, 44°19’22.2”E. 10.02.2010. Leg. Malalatiana Razafindrakoto. Parat ZMUA/035 , 2 clitellate adult eX. Locality and date same as of holotyPe.

Etymology – From the Latin hesPerus = western, refers to the westernmost rence of the Pacific genus Acanthodrilus .

Diagnosis − Length 115−125 mm, diameter 3.5−4 mm. Colour reddish brown. First dorsal Pore in 8/9. Clitellum saddle- shaPed on 13–19. f 14 setal in b. Prostatic Pores Paired on 17, 19. SPermathecal Pores segmental the rim of segments 8, 9 in setal line a. SPermathecae with an elongated o amPoule, and short duct bearing two unstalked acinuous diverticula. G

NEW ACANTHODRILID SPECIES FROM MADAGASCAR ( CLITELLATA) 225

zard large in 5, last Pair of hearts in 13. Calciferous glands lacking, vascular oesoPhagus in 11–13. EXcretory system holoic with J-shaPed nePhridial blad- ders. Holandric, vesicles in 9, 11, 12. Penial setae Present, ectal third slightly bulged, tiP sPoon- shaPed, length ca. 1.4 mm, diameter 0.0375 mm, ornamen- tation scattered serrations.

DescriPtion − Length of the holotyPe 110 mm, width (Postclitellar) 4 mm. Segments No. 152, the last several segments regenerated. ParatyPes 120 mm in length and 3.5−4 mm in diameter, segment No. 304−315. Colour dorsally reddish- brown at the front of the body Prostomium Prolobic, dorsal Pores from 8/9. Segments with slight secondary annulation Setae visible from segment 2, eight Per segment in closely Paired regular rows. Setal for mula after clitellum aa: ab: bc: cd: dd = 7.5:1.25:7.5:1:30 ( Fig. 16). Setae of 17, 19 modified to Penial setae, genital setae lacking. SPermathecal Pores Paired, segmental, on the rim of the segments 8, 9 in line with setae a. Clitellum saddle- shaPed on 13–19. Female Pores in 14 Presetal in the setal line b. Two Pairs of Prostatic Pores in 17 and 19 just at the base of seta b, joined by slightly gibbous seminal grooves. Male Pores minute, eXternally not visible on 18, within the seminal grooves. Genital markings not develoPed ( Fig. 17).

First sePtum visible 5/6, sePta 6/7–8/9 moderately 5/6, 9/10 slightly thickened. One large oesoPhageal gizzard in 5. Dorsal vessel single throughout, last Pair of hearts in 13 EXcretory system holoic with J-shaPed nePhridial bladders ( Fig. 18). Calciferous glands missing, but the oesoPhagus in 11–13 highly vascular. Intestine begins in 16, tyPhlosole lacking. Two Pair of testes and iridescent male funnels in 10, 11. Large seminal vesicles in 9, 11, 12 those in 9 much reduced. One Pair of moderate sized ovaries in 13. Two Pairs of tubular Prostates of similar size in 17 and 19, somewhat coiled and confined to their own segment. Penial setae ca. 1.4 mm in length and 0.0375 mm in diameter. The ectal third slightly bulged, tiP sPoon- shaPed, ornamentation scattered serrations ( Fig. 19). Two Pairs of sPermathecae in 8 and 9. AmPulla elongate, duct short. A Pair of cross- facing, multilo bous diverticulum, sits on the duct just above the sPermathecal Pore ( Fig. 20).

Remarks – The new sPecies is Provisionally Placed into the New Caledoni- an genus Acanthodrilus based on the Presence of J-shaPed nePhridial bladders However, it differs from all Acanthodrilus sPecies by its duPlicate sPermathecal diverticula. To clear its eXact Position further DNA studies are needed.