Nemania feicuiensis Y.H. Pi & Q.R. Li, 2021

Pi, Yin Hui, Long, Si Han, Wu, You Peng, Liu, Li Li, Lin, Yan, Long, Qing De, Kang, Ji Chuan, Kang, Ying Qian, Chang, Chu Rui, Shen, Xiang Chun, Wijayawardene, Nalin N., Zhang, Xu & Li, Qi Rui, 2021, A taxonomic study of Nemania from China, with six new species, MycoKeys 83, pp. 39-67 : 39

Nemania feicuiensis Y.H. Pi & Q.R. Li sp. nov.

Fig. 7

Etymology.

Refers to the collection location, Emerald Park, Chinese name of jade, feicui.

Material examined.

China, Hainan Province, Wuzhishan City, Emerald Park (18°48'9.64"N, 109°31'6.59"E, altitude: 352 m), on dead wood, 14 November 2020, Y.H. Pi, 2020FCGY12-2 (GMB0059, holotype; GMBC0059, ex-type living culture; KUN-HKAS 112698, isotype) .

Description.

Saprobic on the surface of decaying wood. Sexual morph: Stromata effused-pulvinate, superficial, orbicular to ellipsoid or irregularly lobed, 5-27 mm long × 2.5-10 mm wide × 0.3-0.5 mm thick, surface blackish-grey, with inconspicuous perithecial outer mounds, crust weakly carbonaceous; interior black, stromatal tissue between the perithecia carbonaceous; mature stromata lacking KOH extractable pigments. Perithecia 0.3-0.55 mm diam. × 0.25-0.37 mm high, subglobose to depressed-spherical. Ostioles higher than stromatal surface and with openings slightly papillate, black, conspicuous, without encircling disc. Asci 130-180 × 7-11.5 μm (av. = 145 × 9 μm, n = 30), 8-spored, unitunicate, cylindrical, long-stipitate, the spore-bearing parts 65-85 µm long, apically rounded with a J+ apical apparatus, 1-2.5 × 2-3 µm (av. = 1.8 × 2.4 µm, n = 30), long-cylindrical. Ascospores 9.5-13 × 4-7.5 μm (av. = 11 × 6 μm, n = 30), uniseriate, unicellular, ellipsoid or slightly inequilateral, with broadly rounded ends, smooth, brown to dark brown, with a conspicuous, straight, almost spore-length germ slit on the flattened side; lacking a sheath and appendage; perispore indehiscent in 10% KOH. Asexual morph: Undetermined.

Culture characteristics.

Colonies grow slowly on PDA at 25 °C for 2 weeks, with a diameter of 5 cm. Colonies are cotton white in colour, flocculent or velvety, slightly convex, circular, shallow edges, radial, white to light yellow on the reverse, light brown in the middle. Not sporulating on OA nor on PDA.

Other examined material.

China, Hainan Province, Wuzhishan City, Emerald Park (18°47'8.26"N, 109°31'5.34"E, altitude: 426 m), on dead wood, 16 November 2020, Y.H. Pi, 2020FCGY20 (GMB0058), living culture, GMBC0058 .

Notes.

The phylogenetic tree (Fig. 1 ) shows that N. feicuiensis and N. primolutea are closely related (100% ML, 1 BYPP). In morphology, N. feicuiensis differs from N. primolutea in that the latter has luteous stromatal surface and slightly smaller ascospores (10-13 × 4.5-5.5 μm) with narrowly-rounded ends ( Ju et al. 2005). Furthermore, in the multi-gene phylogenetic analysis, N. feicuiensis appeared in a separate branch which is distinct from N. primolutea (Fig. 1 ). Nemania feicuiensis is similar to N. diffusa in stromatal anatomy and ascospores size, but differs by ascospores shape (broadly rounded ends vs. narrowly rounded ends) and the larger perithecia of N. diffusa (0.3-0.6 × 0.4-0.8 mm) ( Ju and Rogers 2002).

Figures

Figure 1. RAxML tree based on analysis of a combined dataset of ITS, α-actin, rpb 2 and β-tubulin sequences from taxa of Nemania and related genera. Bayesian posterior probability (PP) ≥ 0.90 is marked at the node and the maximum likelihood bootstrap support (BS) values greater than ≥ 75 %; a dash (" - ") indicates a value <0.90 (PP) or <75 % (BS). The strain number is indicated after the species name. The here-studied strains are in bold and new species are indicated in red.

Figure 7. Nemania feicuiensis (GMB 0059, holotype) A type material B, C stromata on the surface of host D transverse sections of stromata E longitudinal sections of stromata F-H asci with ascospores I pigments in 10 % KOH J ascospore with indehiscent perispore in 10 % KOH K ascus apical apparatus (stained in Melzer's Reagent) L, M ascospores N, O colonies on PDA (N - upper, O - lower). Scale bars: 0.5 mm (C-E); 10 μm (F-H, J-M).