Artemia salina bioassay

Masi, Marco, Lecce, Roberta Di, Calice, Umberto, Linaldeddu, Benedetto Teodoro, Maddau, Lucia, Superchi, Stefano & Evidente, Antonio, 2022, Diplofuranoxin, a disubstituted dihydrofuranone, was produced together with sphaeropsidin A and epi-sphaeropsidone by Diplodia subglobosa, an emerging ash (Fraxinus excelsior L.) pathogen in Europe, Phytochemistry (113302) 202, pp. 1-6 : 5

4.7. Artemia salina bioassay

All compounds were also assayed on brine shrimp larvae ( Artemia salina L.). The assay was performed in cell culture plates with 24 cells as previously described ( Andolfi et al., 2014). The metabolites were tested at 50 μg/mL. DMSO was used as negative control. Tests were performed in quadruplicate. The percentage of larval mortality was determined after 36 h incubation at 27 ◦ C in the dark.