Amblyseius largoensis ( Muma, 1955 )

Karmakar, Krishna, Bhowmik, Sagarika & Sherpa, Choyang, 2017, Description of five new species and re-description of two species of Amblyseius (Acari: Phytoseiidae) from West Bengal, India, Zootaxa 4311 (1), pp. 39-61 : 51-53

Amblyseius largoensis ( Muma, 1955)

( Figs 30–36 , 57–59 )

Amblyseius largoensis Muma, 1955: 266 .

Amblyseius largoensis .— Ehara & Bhandhufalck, 1977: 67; Gutierrez & Schicha, 1984: 386; Denmark & Muma, 1989: 55; Moraes et al., 2004: 33; Chant & McMurtry, 2007: 78; Demite et al., 2017

Amblyseius (Amblyseius) largoensis .— Gupta, 1986: 51; 1987: 10; 2003: 22.

Female (n = 10). Dorsum ( Fig. 30 ). Dorsal shield 333 (329–338), long and 243 (239–247), wide with seven pairs of solenostomes (gd1, gd2, gd4, gd5, gd6, gd8 and gd9); 17 pairs of dorsal setae and two pairs of sublateral setae: j1 34 (32–38), j3 50 (48–53), j4 7 (5–10), j5 5 (3–7), j6 5 (3–8), J2 8 (6–11), J5 8 (6–11), z2 10 (8–13), z4 8 (6–11), z5 5 (3–6), Z1 9 (7–12), Z4 91 (88–95), Z5 260 (254–266), s4 91 (88–94), S2 10 (8–13), S4 11 (8–13), S5 10 (8– 13), r3 11 (9–14), R1 11(8–13). All setae smooth, j1, j3, are long and s4, Z4, and Z5 are very long.

Peritreme ( Fig. 30 ). Extending forward beyond the bases of j1.

Venter ( Fig. 31 ). All shields smooth sternal shield 63 long and 72 wide at level of setae ST1–ST3 and ST3–ST3 respectively, with three pairs of setae and two pairs of lyrifissures; one pair of setae (ST4) on the metasternal plate; posterior margin of sternal shield wavy and clearly visible. Distances between ST1–ST3 63 (62–65), ST2–ST2 68 (65–71), ST3–ST3 72 (70–73), ST5–ST5 70 (67–73). Two pairs of metapodal shields, primary shield 23 (20–25) long, secondary shield 15 (13–18) long. Ventrianal shield 107 (104–110) long, 43 (40–45) wide at level of ZV2 setae and 60 (57–63) wide at level of anus; with three pairs of pre-anal setae, JV1, JV2 and ZV2 and one pair of large elliptical pre-anal pores. Membrane surrounding ventrianal shield with four pairs of setae ZVl, ZV3, JV4 and JV5 the latter 57 (54–60) long and smooth.

Chelicera ( Figs 32 , 57 ). Fixed digit 31 (28–33) long, with 14 teeth and conspicuous pilus dentilis; movable digit 30 (27–32) long, with three backwardly-directed teeth.

Spermatheca ( Figs 33 , 58 ). Calyx tubular, pipe like 28 (27–32) long. Atrium distinct with flared major duct and very thin long minor duct.

Legs ( Fig. 34 ). Legs IV with three smooth macrosetae with pointed tips and of the following lengths: genu 125 (120–130), tibia 85 (82–88), basitarsus 60 (58–62). Chaetotactic formula of genu II: 1 2/1, 2/1 0; genu III: 1 2/1, 2/ 0 1. Length of leg I 393, (388–398), leg II 313, (309–317), leg III 325, (321–329), leg IV 412, (406–418).

Male (n = 10). A lightly sclerotised mite with 19 pairs of dorsal setae. Idiosomal setal pattern: 10A: 9B/JV– 3,4: ZV–1,3.

Dorsum. Dorsal shield 268 (260–275) long and 193 (185–200) wide and smooth, solenostomes not distinguishable; 17 pairs of setae on dorsal shield and two pairs of setae on unsclerotised cuticle: j1 29 (29–30), j3 45 (43–48), j4 4 (3–5), j5 4 (3–5), j6 4 (3–5), J2 4 (3–5), J5 4 (3–5), z2 5 (4–6), z4 4 (3–5), z5 4 (3–5), Z4 69 (63– 74), Z5 213 (210–215), s4 80 (75–85), S2 9 (8–10), S4 10 (8–11), S5 10 (8–11), r3 12 (10–13), R1 11 (9–12). All setae smooth and setae j1, j3, are long and setae s4, Z4, and Z5 very long.

Peritreme. Extending beyond level of j1.

Venter ( Fig. 35 ). The sternogenital shield has few lateral lines, ventrianal shield reticulated. Sternogenital shield with five pairs of setae and one pair of distinguishable lyrifissures. Ventrianal shield 116 (113–118) long, 115 (110–120) wide at the level of ZV2 setae, 54 (53–55) wide at the level of anus; with three pairs of pre-anal setae, JV1, JV2, and ZV2, one pairs of poroids and two pairs of distinguishable lyrifissures. Unsclerotised membrane surrounding ventrianal shield with one pair of setae, JV5, at level with anal opening.

Legs. Leg IV with three smooth macrosetae, pointed at the tip, of following lengths; genu 83 (80–85), tibia 64 (63–65), basitarsus 51 (49–53). Chaetotactic formula of genu II and genu III are identical to the female. Length of leg I: 335 (320–350), leg II: 254 (250–258), leg III: 258 (250–263), leg IV: 326 (313–338). Chelicera ( Figs 36 , 59 ). Spermatodactyl with an elongated shaft 18 (18–19) long, terminating with a wide toe, 10 (10–11) long.

Specimens examined. 3 females (Acar.lab/BCKV/8052–8053/2016) (Registration number 5439/17 deposited in the NZC, Zoological Survey of India, Kolkata), collected from Hibiscus sp., (Malvacae) at Mohanpur: 22° 56΄ 47̎ N, 88° 32΄ 5̎ E, 9 m above mean sea level, Nadia, West Bengal on 28 September 2016; 5 females (Acar.lab/ BCKV/8074–8078/2016) from Polyanlthia longifolia (Annonaceae) at same locality as earlier specimen on 25 August 2016; 2 females (Acar.lab/BCKV/8080,8083/2016) Indian hog plum, Spondias mangifera (Anacardiaceae) at same locality as earlier specimen on 19 September 2016; 11 females (Acar.lab/BCKV/8088–8098/2016), collected from, Tagar, Tabernamontana coronarea ( Apocynaceae ) at same locality as earlier specimen on 29 July 2016; 10 females (Acar.lab/BCKV/8099–8108/2016), collected from Mango, Mangifera indica , ( Anacardiaceae ) at same locality as earlier specimen on 28 July 2016. 4 females (Acar.lab/BCKV/8109–8112/2016) collected from, Nayantara, Catharanthus roseus (Apocynaceae) at same locality as earlier specimen on 31 July 2016. 6 females (Acar.lab/BCKV/8113–8118/2016) collected from, Codium, Codiaeum variegatum (Euphorbiaceae) at same locality as earlier specimen on 31 July 2016. 4 males (Acar.lab/BCKV/8054,8085–8087/2016), collected from Mango, Mangifera indica , ( Anacardiaceae ) at same locality as earlier specimen on 24 July 2016; 1 male specimen (Acar.lab/BCKV/8055/ 2016) collected from, Indian hog plum, Spondias mangifera (Anacardiaceae) at same locality as earlier specimen on 19 September 2016.

Distribution. Africa: Angola ( Carmona, 1968), Benin ( Zannou et al., 2007), Jamaica ( Denmark & Muma, 1978), Kenya ( Swirski & Ragusa, 1978), Mozambique ( Rodrigues, 1968), Sierra Leone ( Zannou et al., 2007), Tanzania ( El-Banhawy & Abou-Awad, 1990). Asia: China, Guangdong ( Waite & Gerson, 1994), Hainan ( Chen et al., 1980), Taiwan ( Ehara, 1970), India, Andaman Islands ( Gupta,1977a), Andhra Pradesh ( Gupta 1978a), Arunachal Pradesh ( Gupta, 1978b), Assam ( Gupta, 1978b), Bihar ( Gupta & Nahar, 1981), Gujarat ( Gupta, 1977b), Himachal Pradesh ( Gupta et al., 1971), Karnataka ( Gupta, 1978a), Kerala ( Gupta, 1978a), Manipur ( Gupta, 1978b), Meghalaya ( Gupta, 1978b), Myabunder Islands ( Gupta, 1977a), Nagaland ( Gupta, 1978b), Nicobar Islands ( Gupta 1977a), Orissa ( Rao & Rao, 1964), Pondicherry ( Gupta, 1978a), Punjab ( Sadana et al., 1990), Rangat Islands ( Gupta, 1977a), Tamil Nadu ( Gupta 1978a), Tripura ( Gupta, 1978b), Uttar Pradesh (Gupta, 1981), West Bengal ( Gupta, 1970; present study), Indonesia ( Oomen, 1982), Iran ( Daneshvar, 1980), Japan ( Osakabe, 1963), Malaysia ( Ehara, 2002), Philippines ( Corpuz & Rimando, 1966), Singapore ( Corpuz-Raros, 1995), Sri Lanka ( Moraes et al., 2004), Thailand ( Ehara & Bhandhufalck, 1977). Australia ( Schicha, 1981), Fiji ( Collyer, 1980), New Guinea ( Schicha & Gutierrez, 1985), New Zealand ( Collyer, 1964), Vanuatu ( Schicha, 1981), Queensland ( Schicha, 1987). Europe: Israel ( Swirski & Amitai, 1961), Turkey ( Çobanoğlu, 1989), Georgia ( Wainstein & Vartapetov, 1973). North America: US, California ( Muma, 1955), Cook Islands ( Collyer, 1980), Costa Rica ( Chant & Baker, 1965), Cuba ( Rodriguez et al., 1981), Dominican Republic ( Ferragut et al., 2011), Guatemala ( Chant, 1959), Hawaii ( Butler Jr. & Usinger, 1963), Honduras ( Chant & Baker, 1965), Mexico ( Chant, 1959), Samoa ( Schicha, 1981), Saint Kitts ( Garman, 1958), Trinidad ( De Leon, 1967). South America: Brazil, Alagoas (Lawson- Balagbo et al., 2008), Bahia (Oliveira et al., 2007), Colombia ( Zuluaga, 1971), Grande Do Norte ( Furtado et al., 2005), Guyana ( De Leon, 1966), Maranhão ( Lawson-Balagbo et al., 2008), Pará And Paraíba ( Lawson-Balagbo et al., 2008), Pernambuco ( Moraes & Oliveira, 1982), Piauí And Sergipe ( Lawson-Balagbo et al., 2008), Peru ( Aguirre-Gil et al., 2013), Puerto Rico ( De Leon, 1965), Rio Roraima ( Gondim Jr. et al., 2012), São Paulo ( Ehara, 1966), Venezuela ( Aponte & McMurtry, 1993).

Remarks. Amblyseius largoensis ( Muma, 1955) is a widespread and abundant phytoseiid mite species found in the Gangetic Basin of West Bengal in association with many agri-horticultural crops, weeds and forest plants. This species is very clearly identified from its closely related species having vase-shaped ventrianal shield and by its typical characteristic long pipe-like spermatheca.

Figures

FIGURES 30 – 36. Amblyseius largoensis (Muma, 1955) (female). 30. Dorsal view of idiosoma; 31. Ventral view of idiosoma; 32. Chelicera; 33. Spermatheca; 34. Genu, tibia and basitarsus IV; 35. Ventral view of idiosoma (male); 36. Spermatodactyl.

FIGURES 44 – 61. Amblyseius spp., chelicera, spermatheca and spermatodactyl. 44 – 46. A. dahliae sp. nov.; 47 – 49. A. bengalensis sp. nov.; 50 – 51. A. brachycalyx sp. nov.; 52 – 54. A. conulus sp. nov.; 55 – 56. A. parbatabasii sp. nov.; 57 – 59. A. largoensis (Muma, 1955); 60 – 61. A. herbicolus (Chant, 1959).