Cladosporium paris H.W. Shen, Y.X. Xu, H.Y. Su & Z.L. Luo, 2021

Xu, Yue-Xin, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long, Su, Hong-Yan & Hao, Yu-E, 2021, Two new species of Cladosporium from leaf spots of Paris polyphylla in north-western Yunnan Province, China, Biodiversity Data Journal 9, pp. 77224-77224 : 77224

Cladosporium paris H.W. Shen, Y.X. Xu, H.Y. Su & Z.L. Luo sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Yue-Xin Xu; Taxon : scientificName: Cladosporium paris; kingdom: Fungi ; phylum: Ascomycota ; class: Dothideomycetes ; order: Capnodiales ; family: Cladosporiaceae ; genus: Cladosporium ; Location : locationRemarks: China, Yunnan Province, Dali , on diseased leaves of Paris polyphylla; Event : year: 2020; habitat: leaf spots of Paris polyphylla; Record Level : collectionID: 2CL JD 18-2-1; collectionCode: Y-27

Description

Asexual morph: hyphomycetous (Fig. 3 ). Mycelium immersed and superficial, composed of septate, constricted at septa, unbranched, subhyaline, smooth hyphae, 2-6 μm wide. Conidiophores macronematous, 209-285 × 5-8 μm (x̄ = 246.9 × 6.5 μm, n = 15), solitary or in small fascicles, erect to slightly flexuous, sometimes slightly geniculate, non-nodulose, sometimes subnodulose at the uppermost apex, unbranched, 0-6 septate, sometimes slightly constricted at septa, pale to olivaceous-brown, smooth or almost so. Conidiogenous cells cylindrical, sometimes geniculate-sinuous, proliferation of sympodia with up to 5 conidiogenous loci, often crowded at the apex. Conidia 13-21 × 7-12 μm (x̄ = 17 × 9.7 μm, n = 30), solitary or catenate, usually in simple chains, broadly ellipsoid-ovoid, rather pale, pale olivaceous or olivaceous-brown, verruculose, ends usually broadly rounded. Sexual morph: Undetermined.

Culture characteristics: Colonies on PDA attaining 21 mm diam. after 7 d, 40 mm diam. after 14 d and covering the whole Petridish after 30 d, radially folded, furrowed, margin irregularly undulate; reverse olivaceous grey.

Material examined: China, Yunnan Province, Dali, on diseased leaves of Paris polyphylla , 16 October 2020, Y.X. Xu, Y-27. (KUN-HKAS 121701, holotype), ex-type living culture CGMCC 3.20623 = KUNCC 21-10713.

Etymology

" Cladosporium paris " refers to the host genus, Paris .

Distribution

China, Yunnan Province, Dali, on diseased leaves of Paris polyphylla

Notes

Phylogenetic analysis showed that Cladosporium paris is closely related to C. floccosum (Fig. 1 ). Morphologically, our new isolate is distinguished from C. floccosum by its longer conidiophores (up to 285 μm vs. up to 100 μm) and larger conidiogenous cells (13-21 × 7-12 μm vs. 8-15 × 6-8.5 μm). In addition, conidia of C. paris are 0-3 septate, while C. floccosum are 0-1 septate ( Sandoval-Denis et al. 2016). A comparison of the TEF1-α and ACT between C. paris (KUN-HKAS 121701) and C. floccosum (CBS 140463) reveals 3 and 16 nucleotide differences, respectively, which indicates that they are distinct taxa.

Figures

Figure 1. Maximum Likelihood (ML) tree obtained from the combined analysis of ITS, TEF 1 - α and ACT sequences of 161 strains from Cladosporium. The tree is rooted with Toxicocladosporium irritans (CBS 185.58) and T. protearum (CBS 126499). Numbers on the branches represent ML bootstrap support values (MLBS) ≥ 70 %, followed by Bayesian posterior probabilities (PP) ≥ 0.95, lower values are indicated as " - ". Names of species newly described are indicated in red and ex-type strains and reference specimens are indicated in bold. Branch lengths are proportional to distance.

Figure 3. Cladosporium paris (KUN-HKAS 121701, holotype). a Colonies on leaves; b, c Conidiophores; d Conidiophore with conidium; e, f Conidiogenous cells with conidia; g-l Conidia; m Germinating conidium; n, o Culture on PDA from above and reverse. Scale bars: b-d = 50 μm; e, f = 30 μm; g-m = 20 μm.