Albifimbria verrucaria (Alb. & Schwein.) L. Lombard & Crous

Samarakoon, Binu C., Wanasinghe, Dhanushka N., Bhat, Jayarama & Chomnunti, Putarak, 2022, Taxonomy and phylogeny of Smaragdiniseta musae sp. nov. and Albifimbria verrucaria (Hypocreales, Stachybotryaceae) on Musa from Thailand, Biodiversity Data Journal 10, pp. 89360-89360 : 89360

Albifimbria verrucaria (Alb. & Schwein.) L. Lombard & Crous

Materials

Type status: Other material. Occurrence: occurrenceRemarks: Found on a dead leaf of Musa sp.; recordNumber: BNS292; recordedBy: Binu C. Samarakoon; disposition: Living culture: MFLUCC 22-0017; associatedSequences: GenBank: MFLUCC 22-0017: ON563487 (ITS), ON586153 (tub2); Taxon: scientificName: Albifimbria verrucaria (Alb. & Schwein.) L. Lombard & Crous; kingdom: Fungi ; phylum: Ascomycota ; class: Sordariomycetes ; order: Hypocreales ; family: Stachybotryaceae ; genus: Albifimbria ; specificEpithet: verrucaria; taxonRank: species; scientificNameAuthorship: (Alb. & Schwein.) L. Lombard & Crous; Location : continent: Asia ; country: Thailand; stateProvince: Chiang Rai; municipality: Mae Sai ; Identification : identifiedBy: Binu C. Samarakoon; Event : year: 2019; month: June ; day: 25; habitat: Terrestrial ; Record Level : institutionID: MFLU; collectionID: MFLU 22-0048

Description

Saprobic on dead leaves of Musa sp. Sexual morph: Undetermined. Asexual morph: Sporodochia cupulate or discoid, scattered or gregarious, having irregular or rounded outline composed of white marginal hypha, with conidial mass flattened or convex, pale olivaceous-green at an immature stage, black and shiny at maturity, 10-18 × 0.8-3 μm (x̄ = 12.3 × 2.4 μm, n = 20). Stroma rarely well-developed, usually with a thin layer of isodiametric or elongated hyaline cells 15-25 (x̄ = 16.8 μm, n = 10). Setae: not observed. Marginal hyphae hyaline, usually verrucose, septate, curling and coiling, some branched, rounded or blunt at apex, 1.5-4 (x̄ = 3.3, n = 20) in diam. Conidiophores arising from a thin stromatic layer, hyaline, smooth, 30-48 × 1-2 μm (x̄ = 42.2 × 1.7 μm, n = 30) septate, branching repeatedly, forming 2-4 branches at each level, with ultimate branches becoming phialides, which give rise to numerous conidia, conidiophores sometimes also arising from the hyphae. Phialides hyaline, rough-walled 30-48 × 1-2 μm (x̄ = 42.2 × 1.7 μm, n = 30), 3-7 in a whorl, closely packed in a dense parallel layer, cylindrical, hyaline, collate at the base, rounded or acute at apex, sometimes slightly tapering towards apex, 8-16 × 1-3.5 μm (x̄ = 12.2 × 1.8 μm, n = 30). Conidia broadly fusiform, always pointed at one end, mostly truncate or rounded at the other end, hyaline, sometimes sub hyaline, smooth, 5-9.5 × 2-3.5 μm (x̄ = 7.6 × 3.0 μm, n = 30).

Culture characteristics. Conidia germinated on PDA after 12 hours. Colonies growing on PDA reaching 40 mm diam. after 2 weeks in the light conditions at 25°C, mycelium is mostly immersed, not slimy, cottony, white, dense in the middle and comparatively sparse at the periphery, fast-growing. Sporodochia formed after 12 days at the centre as a black uneven ring.

Notes

Based on BLASTn search results of ITS, tub2 and rpb2 sequence data, our stain (MFLUCC 22-0017) showed a high similarity (ITS = 100% tub2 = 100% and rpb2 = 99%), excluding gaps to Albifimbria verrucaria (CBS 188.46). In the multigene phylogeny, MFLUCC 22-0017 grouped with A. verrucaria strains with strong statistical supports (97% ML, 1.00 BYPP) (Fig. 3 ). Morphologically, our collection (Fig. 2 ) is similar to the descriptions of Tulloch (1972) and Lombard et al. (2016). Albifimbria verrucaria has previously been reported from Musa sp. as a saprobe in Venezuela ( Dennis 1970). This is the first report of Albifimbria from Thailand. MFLUCC 22-0017 is the first saprobic A. verrucaria strain found in Thailand. In addition, this is the second report of Albifimbria on Musaceae.

Figures

Figure 2. Albifimbria verrucaria (MFLU 22 - 0048) a, b sporodochia on the host; c, e marginal hyphae; d, f, g conidiophores and phialides; h-l conidia. Scale bars: 400 μm (a, b), 100 μm (i-j), 50 μm (c), 10 μm (d-h), 5 μm (i-l).

Figure 3. Maximum Likelihood tree revealed by RAxML analyses of ITS, rpb 2 and tub 2 sequence data of selected genera of Stachybotryaceae, showing the phylogenetic position of Albifimbria verrucaria (MFLUCC 22 - 0017) and Smaragdiniseta musae (MFLUCC 22 - 0015, MFLUCC 22 - 0016). ML bootstrap supports (≥ 60 %) and Bayesian posterior probabilities (≥ 0.95 BYPP) are given above the nodes, respectively. The tree is rooted with Fusarium sambucinum (CBS 146.95) (Nectriaceae). Strains generated in this study are indicated in bold red. Ex-type strains are indicated in bold black. The scale bar represents the expected number of nucleotide substitutions per site.