Allodiatrype thailandica (R.H. Perera et al.) Konta & K.D. Hyde, Mycosphere 11(1): 253 (2020)

Long, Sihan, Liu, Lili, Pi, Yinhui, Wu, Youpeng, Lin, Yan, Zhang, Xu, Long, Qingde, Kang, Yingqian, Kang, Jichuan, Wijayawardene, Nalin N., Wang, Feng, Shen, Xiangchun & Li, Qirui, 2021, New contributions to Diatrypaceae from karst areas in China, MycoKeys 83, pp. 1-37 : 1

Allodiatrype thailandica (R.H. Perera et al.) Konta & K.D. Hyde, Mycosphere 11(1): 253 (2020)

Fig. 6

Diatrype thailandica ≡ Diatrype thailandica R.H. Pereraet al., Fungal Diversity 78: 1-237, [105] (2016)

Description.

Saprobic on decaying branches of unidentified plant. Sexual morph: Stromata wart-like, pustulate, 0.5-1.8 mm long and 0.8-2.2 mm broad (av. = 1.2 × 1.3 mm, n = 30), about 1 mm thick, 1-18 in a single stroma, visible as black, rounded to irregular in shape on the host surface, erumpent through host bark, solitary to gregarious. Endostroma composed of an outer layer of dark brown to black, small, tightly packed, thin parenchymatous cells and an inner layer of white to yellow, large, loose parenchymal cells. Ostiole opening separately, papillate or apapillate, central. Perithecium immersed in stroma, globose to subglobose, glabrous, with cylindrical short neck, 377-447 μm high, 191-264 μm diam. (av. = 406 × 221 μm, n = 10). Peridium hyaline to dark brown with textura angularis cell layers. Asci 80-113.5 × 6.9-10 μm (av. = 109.3 × 8.5 μm, n = 30), 8-spored, unitunicate, clavate, long-stalked, upper part inflated, apically rounded to truncate, apical rings inamyloid. Ascospores 6-11 × 2-2.5 μm (av. = 8.9 × 2.3 μm, n = 30), irregularly arranged, allantoid, slightly curved, smooth, subhyaline, aseptate, usually with two oil droplets. Asexual morph: undetermined.

Culture characteristics.

Ascospores germinating on PDA within 24 hours. Colonies on PDA, white when young, became pale yellow, irregular in shape, medium dense, flat or effuse, slightly raised, with edge fimbriate, fluffy to fairly fluffy, white from above, reverse side white at margin, pale brown at centre, no pigmentation produced on PDA medium, no conidia observed on PDA or on OA media.

Specimens examined.

China, Yunnan Province, Baoshan City, Lancang River Nature Reserve (24°57'25.35"N, 99°44'22.82"E), on branches of unidentified plant, 2 October 2019. Altitude: 1317 m, Y.H. Pi & Qiong. Zhang, LC 103 (GMB0050, KUN-HKAS 112660, living culture GMBC0050) .

Additional sequences.

GMB0050 (LSU: MW797052).

Note.

The ITS sequence data were subjected to BLAST in NCBI and the results showed that it is 100% similar to Allodiatrype thailandica . Additionally, based on morphological and phylogenetic analyses, this strain was identified as the A. thailandica . The stromata are similar, but the ascospores of GMB0050 are longer and wider than the ascospores of strain MFLUCC 15-3662 (3.8-6.9 × 1-1.4 μm) isolated from the holotype specimen, but it is similar to the strain MFLU 17-0735 (6.5-10.7 × 1.6-2.7 μm) ( Perera et al. 2020). Here, we use the ITS sequence similarity between the new collection and the type strain of Allodiatrype thailandica as basis for identification. A. thailandica has been reported in Thailand in 2016 as Diatrype thailandica and recognised as A. thailandica by Konta et al. (2020). This is the first report of Allodiatrype thailandica from China.

Figures

Figure 6. Allodiatrype thailandica (GMB 0050, new record for China) A stromata on host substrate B, C close-up of stromata D transverse section through ascostroma E vertical section through ascostroma F culture on PDA G section through the ascostroma H ostiolar canal I-K ascospores L-N asci. Scale bars: 20 μm (G); 10 μm (H-N).