Pusulissus coronomensis, Bourgoin & Wang, 2020

Bourgoin, Thierry & Wang, Menglin, 2020, Parahiraciini (Hemiptera, Fulgoromorpha, Issidae): one new genus, two new species and three new subtribes, ZooKeys 997, pp. 69-94 : 69

Pusulissus coronomensis sp. nov. Figs 25-30 , 31-35 , 36-37 , 38-44

Type materials.

Holotype: ♂, China: Guangxi Province, Hezhou, Qichong natural reserve, 24°13'6"N, 110°48'34"E, 180 m, 7 viii 2018, coll. Feilong Yang & Kun Zhao (CWNU) [sequenced specimen]. Paratypes: 1♂, 1♀, same data as holotype (CWNU).

Differential diagnosis.

This new species is very similar to P. phiaoacensis from Vietnam, but differs as follows: 1) Apical margin of the Pcu-A1 lobe on hindwing round (Fig. 30 ), while in P. phiaoacensis it is sinuate (Fig. 7 ); 2) Pair of lateral processes on aedeagus shorter, reaching to the ventral margin of periandrium (Fig. 34 ), while in P. phiaoacensis they surpass the dorsal margin of periandrium (Fig. 11 ); 3) Dorsal margin of female anal tube concave at middle (Fig. 38 ), while in P. phiaoacensis it is nearly straight (Fig. 15 ).

Description.

Length: male (including forewings) (N = 2): 5.1-5.3 mm; female (including forewings) (N = 1): 5.3 mm.

Coloration. General appearance brown (Figs 25 , 26 ). Vertex tawny, with two brown circular markings near the base, midline brown, margins black (Fig. 27 ). Compound eyes brownish dark, supported by tawny callus (Fig. 27 ). Frons brown, dorsal part black (Fig. 28 ); dorsal and lateral margins carinated by black, median carina brown (Fig. 28 ). Frons with two yellowish round markings in middle area, apical and lateral areas distributed with around twelve yellowish tubercles on each side, basal part yellow (Fig. 28 ). Antennae brown (Fig. 28 ). Postclypeus brown mixed with some tawny (Fig. 28 ). Genae tawny (Fig. 26 ). Pronotum tawny, median area with two brownish small impressions (Fig. 27 ), paranotal lobes brown mix with some yellow (Fig. 28 ). Mesonotum tawny (Fig. 27 ). Forewings tawny with veins tawny, the basal part of costal area with some irregular black markings, the middle area from basal third of costal margin extending to the middle of forewing surface also have some irregular black markings, the apical margin with a black linear marking (Fig. 29 ); some specimens with the black markings on forewing almost invisible, but with an obscure large paler round marking at basal third on each side of forewing (Fig. 25 ). Hindwings brown (Fig. 30 ). Legs tawny (Fig. 26 ).

Head and thorax. Vertex 2.3 times wider than long in midline, anterior margin straight, posterior margin angularly concave at middle, but the level shallower than the new species described above (Fig. 27 ). Frons 1.2 times longer in midline than the widest part, 1.2 times wider at widest part than dorsal margin (Fig. 28 ). Pronotum 1.1 times longer in midline than vertex in midline, lateral margins straight and oblique (Fig. 27 ). Mesonotum 2.2 times wider along anterior margin than long in midline (Fig. 27 ). Forewings twice longer at longest part than widest part (Fig. 29 ). Hindwing with apical margin of CuP-Pcu-A1 lobe rounded (Fig. 30 ). Metatibiotarsal formula: 2-(7-8)/(6-8)/2.

Male genitalia. Anal tube in dorsal view mushroom shaped, widest beyond middle, as long in midline as widest part, apical margin rounded (Fig. 32 ), lateral margins roundly convex in dorsal view (Fig. 32 ) and visible in lateral view (Fig. 31 ); anal opening located near the middle of anal tube (Fig. 32 ). Pygofer in lateral view subrectangular, dorsal margin slightly sloping to the posterior, dorso-lateral angle rounded, posterior margin almost parallel with the anterior margin (Fig. 31 ). Gonostylus in lateral view with dorsal margin elevated at the basal third (Fig. 33 ). Capitulum of gonostylus derived after the middle of gonostylus, broad, sharp triangular, directed to anterior; the meniscate processes with one side beyond the base of anterior margin of capitulum and another side beyond the posterior margin of capitulum (Fig. 33 ). In lateral view, periandrium dorso-lateral lobe and ventral lobe rounded apically (Figs 34 , 36 ). In posterior view, ventral lobe of periandrium spinous protruded at middle in apical margin (Fig. 37 ). The lateral processes of aedeagus relatively short, hook-shaped, derived from the middle, directed anteriorly to basal fourth, reaching the ventral margin of periandrium (Figs 34 , 36 ); in ventral view this pair of processes directed downwards (Fig. 35 ).

Female genitalia. Anal tube in dorsal view ovate, 1.1 times longer in midline than widest part, widest before mid length, apical margin slightly concave at middle, lateral margins rounded, anal opening situated slightly below middle (Fig. 38 ). Anterior connective lamina of gonapophysis VIII with two obscure large teeth in the apex but the keeled teeth in outer-lateral margin invisible (Fig. 44 ). Gonocoxa VIII long, quadrangular (Fig. 44 ). Hind margin of sternite VII roundly concave, the median part with a small convex protuberance (Fig. 43 ).

Etymology.

Arbitrary euphonic name referring to the crown (latin ‘corona’) of yellow pustules on the frons.

Note.

Genes sequences were registered in GenBank with the following accession numbers: MT772139 (whole 18S), MT772137 (28S D3-D5), MT772136 (28S D6-D7), MT774094 (COXI) and MT774093 (Cytb). For COXI of 681 bp length, this species differs by 57 bp with P. phiaoacensis and by 50 bp with Pusulissus sp. (Fig. 46 ).

Figures

Figure 46. Bayesian 50 % consensus tree of Parahiraciini species based on combined sequences (18 S, 28 S, COXI, Cytb) with 2 species of Sarimini and 2 species of Hemisphaeriini as outgroup. At each node, values denote posterior probability support.

Figures 25 - 30. Pusulissus coronomensis sp. nov. 25 adult (holotype), dorsal view 26 adult (holotype), lateral view 27 head and thorax (holotype), dorsal view 28 adult (holotype), frontal view 29 forewing (paratype) 30 hindwing (paratype). Scale bars: 1 mm.

Figures 31 - 35. Pusulissus coronomensis sp. nov., male, holotype. 31 genitalia, lateral view 32 anal tube, dorsal view 33 gonostylus, lateral view 34 phallic complex, right lateral view 35 phallic complex, ventral view. Scale bars: 0.2 mm.

Figures 36 - 37. Pusulissus coronomensis sp. nov., male, holotype. 36 phallic complex, right lateral view 37 apex of phallic complex, posterior view. Abbreviations: dll: dorso-lateral lobe of periandrium; Ade: Aedeagus; vl: ventral lobe of periandrium; Adep: Aedeagus processes. Scale bars: 0.2 mm.

Figures 38 - 44. Pusulissus coronomensis sp. nov., female, paratype. 38 anal tube, dorsal view 39 gonoplacs, ventral view 40 gonoplacs, lateral view 41 gonapophysis IX and gonospiculum bridge, dorsal view 42 gonapophysis IX and gonospiculum bridge, lateral view 43 sternite VII, ventral view 44 gonocoxa VIII and gonapophysis VIII, lateral view. Scale bars: 0.2 mm.

Figures 2 - 7. Pusulissus phiaoacensis sp. nov. 2 adult (holotype), dorsal view 3 adult (holotype), lateral view 4 head and thorax (holotype), dorsal view 5 adult (holotype), frontal view 6 forewing (paratype) 7 hindwing (paratype). Scale bars: 1 mm.

Figures 8 - 12. Pusulissus phiaoacensis sp. nov., male, holotype. 8 genitalia, lateral view 9 anal tube, dorsal view 10 gonostylus, lateral view 11 phallic complex, right lateral view 12 phallic complex, ventral view. Scale bars: 0.2 mm.

Figures 15 - 20. Pusulissus phiaoacensis sp. nov., female, paratype. 15 anal tube, dorsal view 16 gonoplac, lateral view 17 gonapophysis IX and gonospiculum bridge, dorsal view 18 gonapophysis IX and gonospiculum bridge, lateral view 19 sternite VII, ventral view 20 gonocoxa VIII and gonapophysis VIII, lateral view. Scale bars: 0.2 mm.