Olla, Casey, 1899

Celli, Nathália Del G. da R., Almeida, Lúcia M., Basílio, Daniel S. & Castro, Camila F., 2021, The way to maturity: taxonomic study on immatures of Southern Brazilian Coccinellini (Coleoptera: Coccinellidae) species important in biological control, Zoologia (e 64154) 38, pp. 1-18 : 14-16

Olla v-nigrum (Mulsant, 1866)

Figs 11, 66–79

Adult ( Figs 66–67). Length 3.91–6.25 mm; width 3.25–5.16 mm. Body rounded, glabrous and convex. Color pattern with very distinct melanic and non-melanic forms. Melanic form with black dorsal color, pronotum with a narrow yellowish-white band on the apical margin, wider at the lateral apexes. Elytra with an orange quadrate spot in the anterior third ( Fig. 67). Non-melanic form with dorsal whitish yellow, pronotum with seven black spots, four of them interconnected forming an “M”, separated and of different sizes. Elytron generally with seven spots: four rounded parallel in a transversal line at the elytral base, near the humeral callus; three parallel, in a transversal line, in the elytral half, the next in the suture, half-moon shaped, and the others rounded; the last spot in 3/4 of the elytron, closer to the lateral margin ( Fig.66). Fourth instar Larva ( Figs 68, 71–79). Length 6.17–7.83 mm; width 1.50–2.00 mm. Body elongated, cylindrical and tapered, with strumae, tegument dark brown with yellow spots well demarcated from the mesonotum, metanotum, and abdominal segments 1 to 8. Head: light yellow with some darker areas, quadrangular with rounded edges and thin light and sparse bristles. Epicranial suture with frontal arms U-shaped. Three sub-conical stemmata, light brown dorsolateral, near the base of the antennae ( Fig. 74). Antennae with 3 antennomeres; antennomere 1 wider and approximately twice as short as antennomere 2; the latter with two apical bristles and a series of papillae; antennomere 3 broad spine shaped with rounded apex ( Fig. 73). Labrum with sparse bristles ( Fig. 71). Mandible well sclerotized with two apical teeth and mola with an acuminate tooth, with a long bristle above the condyle ( Fig. 75). Maxilla with mala (galea + lacinia) largely trapezoidal, rounded at apex, with numerous long curved bristles, and outer margin with thin and short bristles. The last two maxillary palpomeres with sparse bristles; apex of the last palpomere with sensillae ( Fig. 76). Labium with separate ligula, last palpomere with small, sparse and sensilla bristles at apex ( Fig. 76). Thorax: pronotum with two sclerotized, semi-oval, yellow-white plates, with two dark brown longitudinal spots extending to the lateral margins, the inner ones longer than the outer ones, separated by a narrow band ( Fig. 68). External edge of the plates with about 12 long-bristle chalazae; central surface of the plates without chalazae, and very short bristle chalazae in the marginal areas. Meso and metanotum plates dark brown with oval central area light yellow ( Fig. 68). Lateral margin of the plate with yellow spots and about four short-bristled chalazae; anterior and posterior margin without chalazae; dorsally with 2 to 3 chalazae of very short bristles; dorsal surface of the plate with approximately six short-bristle chalazae; laterally with very short bristle chalazae. A pair of circular anterolateral spiracles with lamellae at the opening ( Fig. 78). Legs developed, dark brown ( Fig. 68) with yellow-white sparse setae; with row of dorsal and lateral tibial bristles; ventrally with a bristle brush, most of them tapered and some spatulate at apex near tarsal claw; tarsal claw with a subquadrate basal tooth, long lateral bristle on the external face of the tooth ( Fig. 77). Abdomen: ten segments, S9 facing downwards. S1 to S8 with a pair of circular anterolateral spiracles similar to those of the mesosternum ( Fig. 79). S1 dorsal light brown with yellow circular areas strumae on the sides; and smaller central dark brown areas. From S1 to S8 with yellow spots on the central region; S2 and S3 with two pairs of dark brown strumae; S4 brown with two pairs of strumae on yellow spots; S5 to S8 light brown and two pairs of rounded strumae on dark brown spots, one pair next to central yellow spot and second lateral segment; S9 dark brown ( Fig. 68). Laterally each segment with a pair of whitish yellow strumae ( Fig. 68). Ventrally each segment with transversal rows of bristles. Abdominal dorsal strumae with two large long-bristled chalazae; 6 to 10 small chalazae with short bristles; strumae dorsal-lateral with the same pattern of chalazae and bristles; lateral strumae with approximately 20 chalazae with short-bristles distributed over the entire surface. Tergum of S9 with approximately 100 chalazae with short bristles; most of them on the side edges of the segment.

First instar larva. Length 1.20–2.00 mm; width 0.48–0.64 mm. Body elongated, cylindrical and tapered, with whitish yellow integument. Head, thoracic plates and legs brown. Head similar to fourth instar larva, with epicranial suture, stemmata, antennae and mouthparts similar to the fourth instar. Prothoracic and abdominal strumae with short-bristled chalazae.

Second instar larva. Length 3.33–4.58 mm; width 0.83– 1.17 mm. Head and strumae similar to the first instar.

Third instar larva. Length 4.00– 5.42 mm; width 1.00– 1.42 mm. Larva similar to the fourth instar in structure and color; pronotum with small-defined spots.

Pupa ( Figs 69–70). Length 4.00– 4.83 mm; width 2.83–3.83 mm. Dark brown color on the lateral margins. Oval, convex and truncated anterior region. Pronotum with at least four spots on each side, two on base and two on apex. Mesonotum and metanotum with a big spot on each side. Elytra spot pattern variable. Abdominal segments: S1 without defined spots; S2, S6 and S7 with two rounded spots on each side; S3 to S5 with three spots on each side ( Figs 69–70).