Helvella acetabuloides X.C. Wang & W.Y. Zhuang

Wang, Xin-Cun, Liu, Tie-Zhi, Chen, Shuang-Lin, Li, Yi & Zhuang, Wen-Ying, 2019, A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species, MycoKeys 60, pp. 45-67 : 45

Helvella acetabuloides X.C. Wang & W.Y. Zhuang sp. nov. Figure 3 a–d

Holotype.

CHINA. Inner Mongolia Autonomous Region, Chifeng City, Harqin Banner, Shijia Town, Toudaoyingzi Village, 41°53'20"N, 119°1'1"E, on the ground under Ostryopsis davidiana Decne., 8 Aug 2002, T.Z. Liu & T.H. Liu, HMAS 279703 (= CFSZ 2044).

Etymology.

The species epithet refers to its similarity to H. acetabulum .

Description.

Apothecia stipitate to subsessile, cupulate, margin undulate, involute or revolute, 2.2-4.8 cm high and 2.5-4 cm diam. when dry; hymenium dull brown to reddish-brown when dry, receptacle surface light brown to brown when dry, glabrous; stipe terete or flattened, buff, light yellowish-brown to brown, surface ribbed, 0.5-3 × 0.4-1.3 cm, typically fluted with sharp-edged or rarely blunt ribs, ribs branching at the upper half of receptacle surface, reaching to the edge or ending 1-2 mm from the edge. Ectal excipulum of textura angularis, 75-100 µm thick, cells hyaline, outer cells arranged in chains, 16-21.5 × 7-8 µm. Medullary excipulum of textura intricata, 180-220 µm thick, hyphae hyaline. Asci subcylindrical, tapering and with crozier at base, 8-spored, 235-280 × 15-20 µm. Paraphyses filiform with apical portion very slightly enlarged, septate, hyaline, 4.5-5.5 µm wide at apex and 4-4.5 µm below. Ascospores ellipsoidal, hyaline, smooth, uniguttulate, 14-20 × 10-14.5 µm, median 16.2 × 12.3 µm, Q = 1.2-1.55, median 1.375, n = 50.

Additional specimen examined.

CHINA. Shaanxi Province, Baoji City, Taibai County, Mt. Taibai, 34°1'53"N, 107°25'33"E, alt. 2270 m, on the ground in broad-leaf forest, 26 Jun 1958, J.H. Yu 106, HMAS 23842.

Notes.

Helvella acetabuloides is nested with H. acetabulum , H. arctoalpina , H. costata and H. sichuanensis in Clade 3 ( Figure 1 ). Its hymenium is reddish-brown when dry and different from that of H. acetabulum (brown when dry) and those of H. arctoalpina and H. sichuanensis (black when dry, Harmaja 1977b). The two specimens cited are identical in sequences of Hsp90. Helvella acetabuloides differs from H. acetabulum in 6 bp for Hsp90 (H410, epitype), 14 bp for ITS2 (HMAS 243823) and 17 bp for TEF1 (H133). It is distinguished from H. arctoalpina in 2 bp of Hsp90 (H293, holotype) and 11 bp of TEF1 (H033), from H. costata in 3 bp of Hsp90. It differs from H. sichuanensis in 1 bp of Hsp90, 20 bp of ITS2 and 11 bp of TEF1. PCR amplification of LSU failed.

Figures

Figure 1. Bayesian phylogenetic tree of Helvella and Dissingia inferred from combined Hsp 90, ITS 2, LSU and TEF 1 dataset. Posterior probability values ≥ 0.90 (left) and bootstrap values ≥ 70 % (right) are indicated at nodes.

Figure 3. a-d Helvella acetabuloides: a mature apothecia when dry (CFSZ 2044) b asci (HMAS 23842) c, d ascospores in ascus (c: CFSZ 2044, d: HMAS 23842) e-g Helvella sichuanensis (HMAS 254610): e mature apothecia when dry f mature apothecia when fresh g ascospores in asci h-k Helvella tianshanensis (HMAS 86040): h, i Mature apothecium when dry j asci k ascospores in ascus. Scale bars: 1 cm (a, e); 0.75 cm (h, i); 50 μm (b, g, j); 20 μm (c, d, k).