Tylopilus neofelleus Hongo, J. Jpn. Bot. 42: 154 (1967)

Chakraborty, Dyutiparna, Vizzini, Alfredo & Das, Kanad, 2018, Two new species and one new record of the genus Tylopilus (Boletaceae) from Indian Himalaya with morphological details and phylogenetic estimations, MycoKeys 33, pp. 103-124 : 113-115

Tylopilus neofelleus Hongo, J. Jpn. Bot. 42: 154 (1967) Figs 7, 8

Description.

Pileus 70-120 mm broad, convex to broadly convex; surface distinctly tomentose to subvelvety, dry, subviscid when wet; reddish-brown (8-9F4) when young, rosewood (9C5) to vinaceous-brown (16C5) with maturity, fawn (7E4) towards margin. Pores yellowish-white or cream with a pinkish tinge, orange-grey (6B2) with age; pore 2-3/mm, rounded. Tubes 10-15 mm, adnate-sinuate, white in colour, yellow-brown or orange white with maturity. Stipe 60-100 × 18-22 mm, cylindrical, solid, surface dry, glabrous to subvelutinous, typically distinctly reticulate at apex, reticulation greyish-ruby (12 C–D 4) to dark ruby (12F5); surface pinkish brown to vinaceous or violaceous, dark brown to reddish-brown with maturity. Context chalky white, but pinkish-brown when exposed. Spore print not obtained.

Basidiospores 10 –11.5– 13.5 × 4 –4.6– 5.2 µm (n = 30, Q = 2.05 –2.48– 2.76), ellipsoid to narrowly subfusoid, inequilateral, smooth, thin-walled. Basidia 30-36 × 10-11 µm, 4-spored, clavate, thin-walled, hyaline or pale grey in KOH. Pleurocystidia 35 -66 × 14-24 µm, scattering and numerous, fusoid-ventricose or subclavate, with orange brown contents. Cheilocystidia 33-38 × 9-12 µm, ventricose to fusoid, shorter and smaller than pleurocystidia thin-walled, with orange brown contents. Pileipellis 100-150 µm thick, an ixotrichoderm of suberect, branched, septate hyphae; terminal elements ventricose to fusoid, vaculolated, 28-50 × 12-14 µm, with granular yellowish to brown orange contents in KOH; subterminal elements mostly with incrustations. Stipitipellis 35-65 µm, fertile, composed of basidia and cystidia. Caulocystidia 52-63 × 8-13 µm, fusoid to subfusoid, ventricose to ventricose-rostrate or narrowly cylindrical, content granular. Clamp connection absent in all tissues.

Habitat.

Under Castanopsis sp. in temperate broadleaf forest.

Known distribution.

Japan ( Kawamura 1954; Hongo 1960; Imazeki et al. 1970, 1988; Takahashi 1986; Gelardi et al. 2015), China ( Ying and Zang 1994; Li and Song 2000; Wang et al. 2004; Fu et al. 2006; Wu et al. 2011; Gelardi et al. 2015), Russia ( Vasil’jeva 1978) and New Guinea ( Hongo 1973).

Specimens examined.

India. Sikkim: East district, Fambonglo WLS, 2021 m alt., N27°21'47.5" E88°34'13.2", 26 Aug 2016, D. Chakraborty & K. Das, DC 16-63 (CAL); ibid., D. Chakraborty & K. Das, DC 16-64 (CAL).