Guadarramasoma ramosae Gilgado, Ledesma, Enghoff & Mauriès, 2017

Gilgado, José D., Ledesma, Enrique, Enghoff, Henrik, Mauriès, Jean-Paul & Ortuño, Vicente M., 2017, A new genus and species of Haplobainosomatidae (Diplopoda: Chordeumatida) from the MSS of the Sierra de Guadarrama National Park, central Spain, Zootaxa 4347 (3) : -

Guadarramasoma ramosae Gilgado, Ledesma, Enghoff & Mauriès n. sp.

Type material. Holotype: 1 ♂ (zmuc00039881). SSD 28 . MSS (- 1m). Collado de Valdemartín , Cuerda Larga, Madrid, Spain. 40°47'43.48"N 3°57'20.81"W . 2,160 m asl. Trap working from 03/07/2015 to 06/11/2015. Gilgado , Ledesma, Ortuño et al. leg.

Paratypes:— 4 ♂♂, 4 ♀♀ ( DZAF-UA /VMO). SSD 9 . MSS (- 1m). Dos Hermanas , Peñalara, Madrid, Spain. 40°50'14.60"N 3°57'50.33"W . 2,200 m asl. Trap working from 05/10/2015 to 28/06/2016. Gilgado, Ledesma, Ortuño et al. leg.— 4 ♂♂, 4 ♀♀ same data but ( MNHN Collection Myriapodes-Onychophoresd, DA 292). — 4 ♂♂, 4 ♀♀, same data but ( MNCN 20.07 / 2009-2016). — 6 ♂♂, 7 ♀♀ (zmuc00039882) SSD 1 . MSS (- 1m). Cancho del Río Peces ; Mujer Muerta / Montón de Trigo; Segovia, Spain. 40°49'47.42"N 4° 5'18.95"E , 1,606 m asl. Trap working from 20/05/2015 to 17/09/2015. Gilgado, Ledesma, Ortuño et al. leg. — 1 ♀ (zmuc00039883). SSD 7 . MSS (- 1m). Majada Hambrienta , Peñalara, Segovia, Spain. 40°51'12.19"N 3°57'59.95"W . 1,994 m asl. Trap working from 02/06/2015 to 17/09/2015. Gilgado, Ledesma, Ortuño et al. leg. — 1 ♀ (zmuc00039884) SSD 9 . MSS (- 1m). Dos Hermanas , Peñalara, Madrid, Spain. 40°50'14.60"N 3°57'50.33"W . 2.200 m asl. Trap working from 03/ 06/2015 to 05/10/2015. Gilgado, Ledesma, Ortuño et al. leg. — 1 ♀ (zmuc00039885) SSD 11 . MSS (- 1m). Cerro Ventoso, Siete Picos - La Maliciosa, Madrid, Spain. 40°47'28.36"N 4° 3'22.64"W . 1,876 m asl. Trap working from 09-VI-2015 to 17/09/2015. Gilgado, Ledesma, Ortuño et al. leg. — 1 ♀, 2 juv. (zmuc00039886) SSD 23 . MSS (- 1m). Circo del Pico Nevero, Montes Carpetanos, Madrid, Spain. 40°58'42.11"N 3°50'44.00"W . 2,144 m asl. Trap working from 25/06/2015 to 06/10/2015. Gilgado, Ledesma, Ortuño et al. leg. — 3 ♂♂, 1 ♀, 1 juv. (zmuc00039887) SSD 28 . MSS (- 1m). Collado de Valdemartín , Cuerda Larga, Madrid, Spain. 40°47'43.48"N 3°57'20.81"W . 2,160 m asl. Trap working from 03/07/2015 to 06/11/2015. Gilgado, Ledesma, Ortuño et al. leg. Other studied material: Details of all captured specimens are shown in Table 1.

Etymology. The species is named in honour of Marian Ramos (Museo Nacional de Ciencias Naturales, Madrid), leader of the project ‘Fauna Ibérica’ and supporter of our research on Iberian millipedes.

Description. Colour pale, between ivory and beige ( Fig. 4 ). Head and metazonites, including paraterga, with a slightly darker pigmentation. Adults with 30 ‘segments’ (including collum and telson) and 50 pairs of legs (including the male gonopods). Males slightly longer (30 mm vs 29.5 mm), slenderer (1.4 mm vs 1.5 mm) and with a smaller maximum vertical diameter (1.05 mm vs 1.08 mm) than females.

Male: Head ( Fig. 5A, B ) convex: pilose, with setae of several sizes, some of them visibly longer than the others. Setae on vertex close to the collum arranged in two rows, other setae dispersed, in some regions with higher density, e.g., over the antennal insertions. Maximum width of gnathochilarial stipites 1.2 mm. Between 12 and 18 ommatidia per eye. Region posterior to the antennal insertion with a cobblestone paving-like microsculpture. Labrum with three teeth and 16 supralabral setae ( Fig. 5D ). Gnathochilarial stipites more setose anteriorly than posteriorly. Antennae pilose ( Fig. 5E ) with some visibly longer macrosetae and composed of eight antennomeres, the eighth only visible with SEM and telescoped into the seventh, antennomeres 1–7 measuring 0.08, 0.28, 0.73, 0.43, 0.60, 0.23 and 0.21 mm, respectively.

Collum ( Fig. 5B, C ): Without special modifications, maximum width 0.76 mm, with 3+3 similar-sized macrosetae ( Fig. 5B, C ). Anterior margin in dorsal view with elliptical outline.

Trunk ( Fig. 6A, B ): With conspicuous paranota. Pleurotergites 1–5 similar but increasing in size progressively. Pleurotergite 6 notably wider. Ensuing pleurotergites similar to 6 in width. Metazonites with 3+3 similar-sized macrosetae. On central pleurotergites ( Fig. 6A, B ), the inner seta placed halfway but slightly closer to central suture than to second seta; the second seta a little anteriorly, over the anterior lateral corner of the paranotum; and the third seta more laterally, on the posterior lateral corner of the paranotum. On posteriormost pleurotergites ( Fig. 6C, E ), the setae tend to be more aligned, with the inner seta halfway to the second one and the second and the third ones closer to each other than to the inner one. Posterior margin of metazonites slightly invaginated, forming a very obtuse angle. Prozonites and inferior region of metazonites with a scaly microsculpture as observed with the SEM ( Fig. 5A ).

Legs: Tarsus of legs 1 and 2 with a dense ventral row of long, modified setae ( Fig. 7B, C ). Similar setae also present but scarce and sparse on the tibiae, postfemora and femora of these legs. From legs 3–43, tarsus with a dense field of tongue-shaped modified setae ventrally ( Fig. 7 D-G). Coxae of leg 7 with a distal lobular laterad expansion ( Fig. 7E ). Prefemur of leg 10 slightly swollen ventrally; prefemur of leg 11 with a ventral and distal subtriangular blunt protuberance ( Fig. 7F ). Coxae of legs 10 and 11 with a conspicuous ventral pore ( Fig. 7F–H ). All coxae and sternal knobs with a scaly microsculpture as seen with the SEM but microsculpture not equally well developed and not very conspicuous in general ( Fig. 7A ).

Telson ( Fig. 6C–F ): Epiproct with 3+3 setae and two spinnerets, posterior margin truncate, almost slightly invaginated. Each paraproct with three setae in a vertical line, close to and parallel to the posterior margin, the uppermost seta slightly closer to the second than the second to the third. A semicircular crease close to the hypoproct, reaching the third seta ( Fig. 6D, F ). Hypoproct semicircular, with two long setae.

Anterior gonopods ( Fig. 8A–E ): Remarkably simple. Telopodites (T) long, slightly divergent, curved in their basal region (tb) resembling an elbow as seen in anterior view ( Fig. 8A ). From half its length, also slightly curved posteriad, as seen in lateral view ( Fig. 8C, G, H ). Each telopodite ending in a hook-shaped mace (ti), inner margin of (ti) somewhat serrulate, towards its tip this serrulate structure becoming just a row of thin and short spines. Tip of hook not acute, ending in a group of tiny spine-like expansions, placed above the end of the inner margin and its short spines ( Fig. 8E ). Base of telopodites separated by the wide antero-basal part of the synangiocoxite (A), the latter with a pair of subpyriform masses joined sagittally, each with an oral lobe (a1) and a vestigial wart-like structure (a2); the distal part represented by two divergent posterior long structures (a3), fused in their basal region ( Fig. 8D ), not as long as the telopodite, with a membranous aspect and ending in an acute tip ( Fig. 8B, C ). Adjacent laterally to and not as long as (a3), two subtriangular membranous and coarse structures corresponding to colpocoxites (K) ( Fig. 8B–D ). A true sternite remarkably absent, sternum may be reduced to a membranous strip.

Posterior gonopods (paragonopods): Telopodite constituted by three articles ( Fig. 8F ). Basal article with a lateral coarse region beside the insertion of the second article, and a distal mesal wrinkled prolongation. Second article rounded, with distal setae and a region of coarse microsculpture adjacent to those of first article. Third article inserted laterally and distally on second article, with setae, elongated, sausage-shaped, proximal third slenderer, ending in a blunt tip.

Female: Similar to male, but with some minor differences including a more robust aspect. Row of welldeveloped ventral setae on legs 1 and 2 present, but tongue-shaped setae on other legs lacking. Width of sixth and consecutive pleurotergites notably larger than the previous, although not as conspicuously as in males. Coxae of leg 3 with an anterior subconical expansion ( Fig. 9C, D ).

Vulvae ( Fig. 9A, B ): With no special characters. Operculum (op) setulose. Mesal (vi) and lateral (ve) valves each with ca. 9 setae adjacent to the posterior part of the apodematic groove (ag), their surface coarse as seen with the SEM, except for the apparently more sclerotized anterior zone, with a more serrulated margin surrounding the apodematic groove.

Figures

FIGURE 4. Habitus of a male (A) and a female (B) of Guadarramasoma ramosae gen. & sp. nov. lateral view. Scale bar 2 mm.

FIGURE 5. Details of the morphology of Guadarramasoma ramosae gen. & sp. nov. A) Head in lateral view. B) Head and collum in dorsal view. C) Collum in lateral view. D) Clypeus and gnathochilarium in ventral view. E) Antenna in lateral view, first antennomere missing. Scale bars: A = 0.3 mm; B – D = 0.2 mm; E = 0.5 mm.

FIGURE 6. Details of the morphology of Guadarramasoma ramosae gen. & sp. nov. A) Pleurotergites in lateral view. B) Trunk pleurotergites in dorsal view. C) Telson in laterodorsal view. D) Telson in ventral view. E) Telson in dorsal view. F) Telson in posterior view. Scale bars: A, B = 0.5 mm; C = 0.3 mm; D – F = 0.2 mm.

FIGURE 7. Details of the morphology of Guadarramasoma ramosae gen. & sp. nov. A) Sternal knobs and coxae of midsegments in ventral view. B) Leg 1. C) Leg 2. D) Leg 3. E) Leg 7. F) Leg 11. G) Detail of the tongue-shaped papillae of leg 3. H) Detail of the pores on the coxae of leg 11. Scale bars: A = 0.1 mm; B, C = 0.2 mm; D – F = 0.3 mm; G = 0.03 mm; H = 0.05 mm.

FIGURE 8. Details of the gonopods of Guadarramasoma ramosae gen. & sp. nov. A) Anterior gonopods in anterior view. B) Anterior gonopods in posterolateral view. C) Anterior gonopods in posterolateral view. D) Detail of the anterior gonopods in posterior view. E) Tip of telopodite. F) Posterior gonopod (paragonopod) in posterior view. G) Ventral view of male bearing gonopods in its natural position. H) Lateral image of male bearing gonopods in place. Scale bars: A – C, H = 0.5 mm; D, F = 0.2 mm; E = 0.1 mm; G = 0.3 mm. Abbreviations: T, telopodites; tb, basal part of telopodites; ti, tip of telopodite; A, synangiocoxite; a 1, anterior lobe of synangiocoxite; a 2, vestigial wart-like structure possibly corresponding to median lobe of synangiocoxite; a 3, posterior structures of synangiocoxite; K, colpocoxite.

FIGURE 9. Details of the anatomy of the female of Guadarramasoma ramosae gen. & sp. nov. A) Vulvae in posterior position. B) Left vulva in ventral position. C) Ventral view of an undissected female, showing the space between the second and the third leg where the vulvae are hidden. D) Detail of the coxa of the third pair of legs. Scale bars: A = 0.3 mm; B, C = 0.2 mm; D = 0.1 mm. Abbreviations: Op, operculum; vi, mesal valve; ve, lateral valve; ag, apodematic groove.