Dioszegia foliicola Y.Z. Qiao & F.L. Hui, 2024

Qiao, Ya-Zhuo, Liu, Shan, Niu, Qiu-Hong & Hui, Feng-Li, 2024, Three new Dioszegia species (Bulleribasidiaceae, Tremellales) discovered in the phylloplane in China, MycoKeys 101, pp. 313-328 : 313

Dioszegia foliicola Y.Z. Qiao & F.L. Hui sp. nov.

Fig. 2B

Etymology.

The specific epithet foliicola refers to the type strain isolated from a leaf.

Typus.

China, Guizhou Province, Guiyang City, Guiyang Botanical Garden, in the phylloplane of Salvia sp., September 2022, L. Zhang and F.L. Hui, NYUN 229182 (holotype GDMCC 2.316T preserved as a metabolically inactive state, culture ex-type PYCC 9939 and CICC 33571).

Description.

On YM agar, after 7 days at 20 °C, the streak culture is orange, butyrous, smooth. The margin is entire. On YM agar, after 7 days at 20 °C, cells are ovoid and ellipsoidal, 3.9-4.8 × 4.8-7.9 μm and single, budding is polar. After 1 month at 20 °C, a ring and sediment are present. In Dalmau plate culture on corn meal agar, hyphae and pseudohyphae are not formed. Sexual structures are not observed for individual strains and strain pairs on PDA, CM agar and YCBS agar for two months. Ballistoconidia are not produced on CM agar after two weeks at 20 °C. Glucose fermentation is absent. Glucose, sucrose, raffinose, melibiose, galactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5-keto-D-gluconate, D-ribose, galactitol, D-mannitol, succinate, D-gluconate, N-acetyl-D-glucosamine, 2-keto-D-gluconate and D-glucuronate are assimilated as carbon sources. Inulin, lactose, methanol, ethanol, glycerol, erythritol, ribitol, D-glucitol, myo-inositol, DL-lactate, citrate, D-glucosamine, and glucono-1,5-lactone are not assimilated. Nitrite and L-lysine are assimilated as nitrogen sources. Nitrate, ethylamine, and cadaverine are not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China, Guizhou Province, Guiyang City, Guiyang Botanical Garden, in the phylloplane of Broussonetia papyrifera , September 2022, L. Zhang and F.L. Hui, NYUN 229188 and China, Henan Province, Nanyang City, Baotianman Nature Reserve, in the phylloplane from an unidentified tree, October 2022, J.Z. Li, NYUN 2211140.

GenBank accession numbers.

Holotype GDMCC 2.316T (ITS: OP566887, D1/D2: OP566888); additional strains NYUN 229188 (ITS: OP566890, D1/D2: OP566889) and NYUN 2211140 (ITS: OR863956, D1/D2: OR863957).

Note.

Dioszegia foliicola sp. nov. can be physiologically differentiated from its closest known species D. maotaiensis ( Li et al. 2020) by its inability to assimilate inulin and citrate, its ability to assimilate methyl-α-D-glucoside, salicin, L-sorbose, D-ribose, galactitol, and D-mannitol, and its capacity to grow at 30 °C.

Figures

Figure 2. Vegetative cells of Dioszegia guizhouensis sp. nov. NYNU 22985 T (A), Dioszegia foliicola sp. nov. NYUN 229182 T (B), and Dioszegia aurantia sp. nov. NYNU 229189 T (C) following growth in YM broth for 7 days at 20 ° C. Scale bars: 10 μm.