Parastagonospora yunnanensis Y. Gao, H. Gui & K. D. Hyde, 2024

Gao, Ying, Zhong, Tingfang, Eungwanichayapant, Prapassorn Damrongkool, Jayawardena, Ruvishika S., Hyde, Kevin D., Faraj, Turki Kh., Wanasinghe, Dhanushka N. & Gui, Heng, 2024, Two new species of Parastagonospora and a new species of Phaeoseptoriella (Phaeosphaeriaceae, Pleosporales) from grasslands in Yunnan Province, China, MycoKeys 109, pp. 239-263 : 239-263

Parastagonospora yunnanensis Y. Gao, H. Gui & K. D. Hyde sp. nov.

Fig. 2

Etymology.

The specific epithet “ yunnanensis ” refers to Yunnan Province, where the holotype was collected.

Holotype.

HKAS 128771 .

Description.

Saprobic on decaying stem of Lolium perenne ( Poaceae ). Sexual morph: Undetermined. Asexual morph: Conidiomata 35–45 µm high, 120–140 μm diam. (x – = 40.6 × 132.7 μm, n = 10), solitary, flattened, subglobose to irregular oval, brown to dark brown spots, immersed in the epidermis of the host, ostiolate. Conidiomata wall 4–13 µm wide (x – = 9 μm, n = 25), composed of brown cells of textura angularis, with an inner layer comprising hyaline cells. Conidiogenous cells (3.2 –) 3.5–4.7 (– 5.3) × (3.4 –) 4–5.3 (– 6.1) μm (x – = 4 ± 0.57 × 4.77 ± 0.59 μm, n = 30), hyaline, ampulliform or globose to subglobose, smooth-walled. Conidia (16.2 –) 18–20 (– 20.4) × (3 –) 3.2–3.7 (– 4) μm (x – = 19 ± 1.1 × 3.4 ± 0.24 μm, n = 35), hyaline, 0–1 - septate, cylindrical to subcylindrical, rounded at apex, slightly truncate at base, guttulate, smooth-walled.

Culture characteristics.

Conidia germinated on PDA within 24 hours, and a germ tube was initially produced from the ends of the conidia. Colonies on PDA reaching 20 mm in 3 weeks at room temperature (25–27 ° C), regular, floccose, white from the above and light grey from the centre and below, smooth with a filamentous edge.

Material examined.

China • Yunnan Province, Zhaotong City , (26 ° 56 ' 39 " N, 103 ° 8 ' 53 " E), on a decaying stem of Lolium perenne ( Poaceae ), 25 August 2022, Ying Gao, QG 69 A ( HKAS 128771 , holotype), ex-type ( CGMCC 3.24527 ) • ibid. QG 69 B ( HKAS 128772 , paratype), ex-paratype ( CGMCC 3.24528 ) • ibid. QG 71 A ( HKAS 128773 ), culture ( CGMCC 3.24529 ) • ibid. QG 71 B ( HKAS 128774 ), living culture ( CGMCC 3.24530 ); ibid. • Qujing City , (26 ° 21 ' 31 " N, 103 ° 14 ' 13 " E), on a decaying stem of Lolium perenne ( Poaceae ), 27 August 2022, Ying Gao, QG 19 A ( HKAS 128799 ), living culture ( CGMCC 3.24511 ) • ibid. QG 19 B ( HKAS 128800 ), living culture ( CGMCC 3.24512 ).

Notes.

Parastagonospora yunnanensis is introduced as a new species based on its distinct morphology and phylogenetic analysis of combined SSU, LSU, ITS, tef 1 - α, and rpb 2 datasets. We have collected six isolates of this fungus from both the Qujing and Zhaotong regions. Parastagonospora yunnanensis is phylogenetically related to Parastagonospora elymi (KUMCC 16-0125). Parastagonospora elymi was introduced as a saprobic fungus from Elymus repens in Russia, the asexual morph of Parastagonospora elymi has not been determined ( Goonasekara et al. 2019). In addition, the ITS pairwise nucleotide comparison of these species showed 18 / 497 bp differences (3.62 %, with 2 gaps), the comparison of base pairs in LSU showed 0.36 % differences (3 / 834 bp, without gaps), SSU, tef 1 - α, and rpb 2 of Parastagonospora elymi were not provided.

Figures

Figure 2. Asexual morph of Parastagonospora yunnanensis (HKAS 128771, holotype) on a dead stalk of Lolium perenne a, b conidiomata on the host c, d vertical section of conidiomata e conidioma wall f – h conidiogenous cells arise from the wall and develop conidia i – l conidia m germinating conidium n cultures on PDA from above o cultures on PDA from the reverse. Scale bars: 30 μm (c, d); 10 μm (e, h – m); 5 μm (f, g).