Diaporthe hubeiensis Dissanayake, X.H. Li & K.D. Hyde

Yang, Qin, Tang, Jie & Zhou, Guo Y., 2021, Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species, MycoKeys 84, pp. 15-33 : 15

Diaporthe hubeiensis Dissanayake, X.H. Li & K.D. Hyde

Figure 3

Diaporthe hubeiensis Manawasinghe, Dissanayake, Li, Liu, Wanasinghe, Xu, Zhao, Zhang, Zhou, Hyde, Brooks & Yan, Frontiers in Microbiology 10(no. 1936): 20 (2019)

Description.

Asexual morph: pycnidia on PDA in culture, 700-885 μm in diam., superficial, scattered, dark brown to black, globose or subglobose. Conidiophores reduced to conidiogenous cells. Conidiogenous cells (6.5-)7-10(-11.5) × 2-3.5 μm (n = 30), aseptate, cylindrical, phiailidic, straight or slightly curved. Alpha conidia 5.8-8(-8.5) × 2.5-3.2 μm (n = 30), aseptate, hyaline, ellipsoidal to cylindrical, biguttulate, blunt at both ends. Beta conidia not observed.

Culture characters.

Culture incubated on PDA at 25 °C, originally flat with white felted aerial mycelium, becoming dark brown mycelium due to pigment formation, conidiomata irregularly distributed over agar surface after 20 days.

Specimens examined.

China. Hunan Province: Zhuzhou City , on leaves of Camellia oleifera , 27°2'35"N, 113°19'20"E, 14 Aug. 2020, Q. Yang (CSUFT019; living cultures: HNZZ019 and HNZZ009) .

Notes.

Diaporthe hubeiensis was originally described as pathogen of grapevines in Hubei Province, China ( Manawasinghe et al. 2019). In the present study, two isolates (HNZZ019 and HNZZ009) are closely related to D. hubeiensis in the combined phylogenetic tree (Fig. 1 ). The differences of nucleotides in the concatenated alignment (1/460 in ITS, 3/458 in cal, 1/320 in his3 and 3/433 in tub2) are minor. Morphological comparison indicated that the isolates were similar to D. hubeiensis by the size of alpha conidia. We therefore identify the isolates as belonging to D. hubeiensis .

Figures

Figure 1. Phylogram of Diaporthe resulting from a maximum likelihood analysis based on combined ITS, cal, his 3, tef 1 and tub 2. Numbers above the branches indicate ML bootstraps (left, ML BS ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.75). The tree is rooted with Diaporthella corylina. Isolates in current study are in blue. " - " indicates ML BS <50 % or BI PP <0.75.

Figure 3. Diaporthe hubeiensis (HNZZ 019) A Culture on PDA B conidiomata C conidiogenous cells D alpha conidia. Scale bars: 500 μm (B); 10 μm (C-D).