Mixophrya, Foissner and Berger, 2021

Min, Kyu-Seok Chae and Gi-Sik, 2023, New record of three hypotrich soil ciliates (Ciliophora: Hypotricha) from South Korea: Oxytricha multilineata, Mixophrya pantanalensis pantanalensis and Caudiurostyla sinensis, Journal of Species Research 12 (4), pp. 307-312 : 308-310

Mixophrya pantanalensis pantanalensis Foissner and Berger, 2021 ( Fig. 2 ) ḵḓšAEÜAEệ (ljḡ)

Mixophrya pantanalensis pantanalensis Foissner and

Berger, 2021: p. 248, Figs. 76 - 78, Table 32.

Material examined. Soil sample from Yeoju­si, Gyeonggi­do, Korea (37°18 ʹ 26.8 ʺ N, 127°41 ʹ 02.4 ʺ E), collect­

ed by Kyu­Seok Chae on 22 September 2020.

Diagnosis. Cell size about 140 - 245 × 70 - 126 μm in protargol preparations; flexible body; 48 - 62 adoral zone of membranelles; 2 macronuclear nodules with about 1 - 9 micronuclei; contractile vacuole at the left of midbody; 5 - 7 lithosomes; 3 frontal cirri; 1 buccal cirrus; 4 cirri in frontoventral cirri; 3 postoral ventral cirri; 2 pretransverse and 5 transverse cirri; 1 left (35 - 54 cirri) and 1 right (40 - 53 cirri) marginal cirral row; 6 dorsal kineties; 3 caudal cirri.

Distribution. Brazil and South Korea.

Remarks. The Korean population of M. p. pantanalensis morphologically corresponds to the type population (Brazilian population) in terms of ventral and dorsal ciliature ( Foissner and Berger, 2021). However, the Korean population differs from the Brazilian population in terms of body size in vivo (about 250 × 130 vs. about 370 × 155 μm) ( Foissner and Berger, 2021). Mixophrya pantanalensis pantanalensis differs from M. gigantea in the number of dorsal kineties (6 vs. 4) and the present of lithosomes (present vs. absent). Mixophrya pantanalensis pantanalensis differs from M. p. australiensis by present of lithosomes (present vs. absent).

The 18S rDNA sequence of M. p. pantanalensis was 3028 bp in length (GenBank accession number: OQ919 808). The 18S rDNA gene sequence of M. p. pantanalensis was compared with that of species within the Oxytrichidae family because no sequences were available for the same species or genus. The species with the lowest pairwise distance value was Urosoma emarginata (MH393886). The interspecific genetic distance between M. p. pantanalensis and U. emarginata was 0.011 (19 bp difference of 1660 bp) ( Table 1).

Voucher slides. One slide with protargol­impregnated specimens was deposited at the National Institute of Biological Resources (NIBRPR0000111059).

Figures

Fig. 2. Mixophrya pantanalensis pantanalensis from life (A-C) and after protargol impregnation (D, E). A, Ventral view showing lithosomes (arrows); B, Cytoplasm showing cortical granule (arrows) and mitochondria (arrowheads); C, Cytoplasm showing surrounded lithosome (arrow) from crystalline structures and masses of granules (arrowhead); D, E, Ventral and dorsal view of typical specimen; AZM, adoral zone of membranelles; BC, buccal cirrus; CC, caudal cirri; FC, frontal cirri; LMR, left marginal cirral row; PTC, pretransverse cirri; RMR, right marginal cirral row; TC, transverse cirri; 1-6, dorsal kineties 1-6. Scale bars=10μm (B, C); 50μm (A, D, E).