Neodendryphiella michoacanensis Iturrieta-Gonzalez , Dania Garcia & Gene

Iturrieta-Gonzalez, Isabel, Gene, Josepa, Guarro, Josep, Castaneda-Ruiz, Rafael F. & Garcia, Dania, 2018, Neodendryphiella, a novel genus of the Dictyosporiaceae (Pleosporales), MycoKeys 37, pp. 19-38 : 19

Neodendryphiella michoacanensis Iturrieta-Gonzalez, Dania Garcia & Gene sp. nov. Fig. 3

Etymology.

Name refers to Michoacán, the geographical area where the fungus was collected.

Type.

Mexico, Michoacán, Villa Jiménez, from soil, Sept. 2016, E. Rodriguez-Andrade (holotype CBS H-23478; culture ex-type CBS 144323 = FMR 16098).

Description.

Mycelium superficial and immersed, composed of septate, branched, smooth to verruculose and hyaline to pale brown hyphae of 1-3 μm wide. Conidiophores semi-macronematous to macronematous, mononematous, erect or slightly flexuous, slightly branched, 1-13 septate, cylindrical or slightly swollen in the conidiogenous loci, 44-280 × 2-4 μm, brown, usually darker toward the base, smooth or verruculose, verrucose at the base. Conidiogenous terminal and intercalary, cylindrical or clavate, 11-62 × 3-5 μm, with up to 3 pores. Ramoconidia (0-)1-septate, with up to 4 terminal or subterminal pores, pale brown, smooth to verruculose, cylindrical, subcylindrical, to slightly clavate, with more or less rounded apex and truncate base, 12-23 × 3 –4(– 5) μm. Conidia catenate, with up to 4 conidia in the terminal unbranched part, (0 –)1(– 2)-septate, some slightly constricted at the septum, pale brown, verruculose to verrucose, ellipsoidal or subcylindrical, 5 –16(– 18) × 3-6 μm.

Culture characteristics

(14 d at 25 °C). Colonies on PDA reaching 34 mm diam., slightly umbonate, velvety, olive brown (4F6/4E8), with slightly fimbriate margin; reverse dark green (30F8) to black. On PCA attaining 42 mm diam., flat, granular, olive brown (4F8), aerial mycelium scarce, fimbriate margin; reverse dark green to olive brown (30F8/4F8). On OA reaching 48 mm diam., flat, granular, yellowish-brown to olive (5F4/3D4), aerial mycelium scarce, with a regular margin; reverse brownish-grey to greyish-yellow (4D2/3B6). On SNA attaining 22 mm diam., flat, slightly granular, olive brown (4F8), aerial mycelium scarce, with slightly fimbriate margin; reverse dark green (30F8) to black. On MEA reaching 13-15 mm diam., slightly umbonate, flat towards the periphery, velvety, yellowish-grey to olive (3C2/3F8), with white irregular margin; reverse olive grey to dark green (3E2/30F8).

Cardinal temperature for growth.

Optimum 25 °C, maximum 30 °C, minimum 10 °C.

Distribution.

México.

Notes.

Neodendryphiella michoacanensis morphologically resembles N. mali , in its conidiogenous apparatus with 0-1-septate ramoconidia, but the latter differs by having longer conidiophores (up to 385 μm), terminal conidial chains with up to 10 conidia and its conidia are 0-1-septate and smaller (4-15 × 3-5 μm). Neodendryphiella michoacanensis also resembles D. uniseptata in their conidial morphology, but ramoconidia of the latter species are often aseptate and can be up to 30 μm long ( Matsushima 1971). Dendryphiella uniseptata is only known from the type material, which was collected in Honiara (Japan) and no ex-type culture was preserved. This species was considered a synonym of D. infuscans by Matsushima (1975) but not accepted by Liu et al. (2017).