Tripyla setifera Bütschli 1873

Xu, Yu Mei, Zhao, Zeng Qi, Davies, Kerrie A. & Wang, Jian Ming, 2017, Tripylella australis sp. nov. and Tripyla setifera Bütschli 1873 (Nematoda: Triplonchida: Tripylidae) from Australia, Zootaxa 4250 (1), pp. 55-66 : 60-64

Re-description of Tripyla setifera Bütschli 1873

( Figs 3 & 4 )

Measurements. Table 2.

Description. Female. (n = 5). Body ventrally arcuate when heat fixed, posterior part more curved than anterior ( Figs 3 A & 4A). Cuticle 1.5–1.9 (1.6 ± 0.2) µm thick at head region, 3.6–4.8 (4.1 ± 0.5) µm thick at vulva region, and 3.4–4.3 (3.7 ± 0.4) µm thick at anus region. Cuticular annulation 1.6–1.9 (1.8 ± 0.1) µm thick. Body pores not observed in the samples with the microscopes. Maximum body diameter 34–39 (36 ± 2.3) µm at level of vulva. Head region rounded, smooth, anterior end slightly flattened, continuous with body contour, narrower than adjacent posterior body, 18–20 (19 ± 0.2) µm wide, 3–4 (3.5 ± 0.3) µm height ( Figs 3 C, 4C & 4D). Inner labial papillae distinct. Outer labial setae and cephalic setae arranged in two separate whorls. Outer labial setae conoid, 5–6.5 (5.9 ± 0.6) µm long, four short cephalic setae, 2–2.5 (2.3 ± 0.2) µm long, situated 5–6 (5.5 ± 0.4) µm posterior to outer labial setae ( Fig. 3 C). Stomatal chamber large with a large wedge-shaped dorsal tooth ( Fig. 4 B), 19–22 (21 ± 1.1) µm from anterior end, and two tiny subventral teeth situated 5–6 (5.3 ± 0.7) µm anterior to dorsal tooth. Amphid stirrup-shaped with transverse oval opening, 4–6 (5.1 ± 1.1) µm wide, located 14–18 (16 ± 1.8) µm from anterior end ( Fig. 4 C). Excretory pore 61–67 (64 ± 4.6) µm from anterior end or 25–27 (26 ± 0.9) % of the pharyngeal length ( Fig. 4 D). Nerve ring 76–86 (80 ± 4.9) µm from anterior end or 30–36 (33 ± 3.2) % of the pharyngeal length. Pharynx cylindrical and muscular, 236–260 (247 ± 9.5) µm long. Three prominent cells situated at junction of pharynx and intestine ( Fig. 3 D). Female genital system amphidelphic, gonads lying ventro-lateral to intestine, the anterior part 84–111 (97 ± 10) µm long, comprising 9–10 (9.6 ± 0.7) % of body length, the posterior part 72–126 (94 ± 23) µm long, comprising 8–13 (9.3 ± 2.4) % of body length. Eggs present in some reproductive systems, 69–89 (78 ± 10) µm long and 19–20 (20 ± 0.4) µm wide ( Fig. 4 G). Vulva simple, lips flat ( Fig. 4 F). Vagina occupying 39–47 (42 ± 3.4) % of corresponding body diameter. No distinct prerectum. Rectum 15–24 (20 ± 3.8) µm long. Tail bent ventrally, narrowing evenly, spinneret 5–6 (5.7 ± 0.4) µm long ( Fig. 4 I). Three tandem caudal glands arranged in a row ( Fig. 3 E).

Male. Not found.

Habitat and locality. Five females and one juvenile, WINC no. 0 17625, collected from soil under Prunus persica , a woody weed in a dense stringy bark forest with tree ferns, very steep and hilly, wet gravelly sandy soil and clay from Lind National Park, East Gippsland, Victoria, Australia (37.56666565°S, 148.9667.53E, altitude ca 120 m asl). Coll. T. Feckner, 16. x. 1987.

Character Female Male

This study Poland Korea Korea Hungary Iran Poland Korea Korea Hungary Iran Brzeski & Brzeski & Winiszewska- Andrássy Asghari et Brzeski & Brzeski & Winiszewsk Andrássy Asghari et Winiszewska- Winiszewska- Ślipiṅska et al. 2007 al. 2017 Winiszewsk Winiszewsk a-Ślipiṅska 2007 al. 2017 Ślipiṅska Ślipiṅska 1993 2000 a-Ślipiṅska a-Ślipiṅska et al. 2000

1993 1993 1993

5 28 2 13 N/A 14 20 1 28 N/A 7

28 ± 1.7 25—28 23—25 22—25 24—31 21—37 25—37 26 26—31 28—35 26—38 (25—30)

4.1 ± 0.1 4.2—5.8 4.5—4.7 3.7—4.5 3.7—5.6 4.4—5.4 4.3—5.6 4.8 4.1—4.8 4.5—5.4 4.6—5.8 (3.9—4.2)

8.0 ± 0.4 6.2—8.0 7.4—7.7 6.8—8.6 6.4—9.0 6.3—7.8 6.0—7.9 7.3 6.1—7.3 6.0—8.6 6.7—7.3 (7.4—8.4)

5.0 ± 0.3 4.5—6.8 4.2—4.4 3.9—5.3 4.0—6.5 4.7—6.9 4.2—6.2 4.4 4.0—5.7 4.2—6.6 4.3—6.7 (4.7—5.5)

57 ± 1.5 53—57 58—59 57—61 52—60 53—58 — — — — — (55—59)

length 1009 ± 43 930—1730 1000—1020 940—1110 1000—1500 1040—1379 1090—1760 960 940—1140 1100—1500 1146—1541

(952—1069)

diameter 19.2 ± 0.8 20—29 21 23—25 20—28 17—20 19—27 19 21—25 20—28 N/A

(18—20)

Dorsal tooth- 20.6 ± 1.1 18—26 19 20—22 N/A 18—21 18—26 18 19—22 N/A 18—21

anterior (19—22)

Excretory pore- 64.0 ± 4.6 63—100 64—65 60—74 N/A 80—106 60—91 58 60—71 N/A N/A

anterior (60—67)

Pharynx length 247 ± 9.5 221—342 218—220 230—253 N/A 234—257 221—336 200 215—249 N/A 237—266

(236—259)

length 126 ± 7.1 115—250 131—137 126—154 150—240 149—199 139—218 132 133—187 N/A 156—227

(116—134)

Spicule — — — — — — 28—43 32 30—34 32—40 31—38 A means the data was not included in the original paper.

Remarks. Tripyla setifera is recorded from Europe (Netherlands, Belgium, Germany, Denmark, Baltic, Sweden, Poland, Austria, Slovakia, Hungary, Serbia, Bulgaria, Spain, Italy, Estonia, Russia, Portugal), Africa (Zaire), Asia (Nepal, Korea, Russia Far East, China, Iran) and the Arctic ( Brzeski & Winiszewska-Ślipińska 1993; Winiszewska-Ślipińska et al. 2000; Andrássy 2007; Vieira et al. 2010; Holovachov 2014; Asghari et al. 2017). The published morphometrics of Tripyla setifera are listed in Table 2. This is a new record of the species, and the first official record of Tripyla for Australia. The Australian collection of T. setifera is close to the Hungarian populations reported by Andrássy (2007) except for body length (respectively, L=952–1069 vs 1000–1500 µm). These may be geographical differences.

Figures

FIGURE 3. Line drawing of Tripyla setifera. A: Whole body; B: Vulva; C: Anterior region; D. Oesophagus-intestine junction; E: Tail shape. Scale bars: A, B, C, E = 50 µm; D = 25 µm.

FIGURE 4. Light micrographs of Tripyla setifera. A: Whole body shape; B: Anterior region, showing outer labial setae, dorsal tooth (arrowed); C: Anterior region, amphid arrowed; D: Anterior region, excretory pore arrowed; E: Pharynx-intestine junction; F: Vulva in lateral view; G: Egg; H: Tail; I: Terminus spinneret.