Roridomyces pruinosoviscidus (Corner) Blanco-Dios, 2020

Lu, Wenhua, Alfagham, Alanoud T., Tibpromma, Saowaluck, Suwannarach, Nakarin, Kumla, Jaturong, Dai, Dong-Qin, Elgorban, Abdallah M., Chukeatirote, Ekachai & Karunarathna, Samantha C., 2025, Hidden in the daylight: A polyphasic approach reveals two bioluminescent Mycenaceae species from Yunnan Province, China (Basidiomycota, Agaricales), Biodiversity Data Journal 13, pp. e 168858-e 168858 : e168858-

Roridomyces pruinosoviscidus (Corner) Blanco-Dios, 2020

Materials

Type status: Other material. Occurrence: occurrenceID: 62EA86DA-A16C-5000-B38F-009ECD731B09; Taxon: kingdom: Fungi; phylum: Basidiomycota; class: Agaricomycetes; order: Agaricales ; family: Mycenaceae ; genus: Roridomyces ; specificEpithet: pruinosoviscidus ; taxonRank: species; Location: country: China; stateProvince: Yunnan; county: Xishuangbanna; locality: Xishuangbanna Primitive Forest Park ; verbatimElevation: 1280 m; verbatimLatitude: 21°59′32″N; verbatimLongitude: 100°54′44″E; Identification: identifiedBy: Wenhua Lu; Event: year: 2023; month: July; day: 7; Record Level: institutionID: Guizhou Medical University Herbarium (GMB); institutionCode: GMB 1054 ; collectionCode: LWH 2307 ; informationWithheld: other material, GMB 1056 , living culture GMBCC 1068 ; GenBank accession numbers ITS: PQ 373881 ( GMB 1054 ), PQ 373882 ( GMB 1056 ), PQ 373884 ( GMBCC 1068 ); LSU: PQ 373887 ( GMB 1054 ), PQ 373888 ( GMB 1056 ), PQ 373890 ( GMBCC 1068 )

Description

Basidiomata small. Pileus 2.5–11 mm diam., hemispherical to parabolic when young, expanding to flatten with age, white, with a central depression, brownish-orange (5 C 5); surface dull, dry, striate, pruinose; margin decurved, thin, orange-white (5 A 2, 6 A 2) to yellowish-white (2 A 2). Context thin, white. Lamellae decurrent, distant, with 1–3 series of rugose lamellulae; orange-white (5 A 2). Stipe 5–50 × 1–2 mm, central, equal, round and hollow; surface viscid to glutinous, shiny; apex milk-white (1 A 1–2), then changing to pale yellow (4 A 3), orange, white (5 A 2) to golden yellow (5 B 7); base hygrophanous, brownish-orange (5 C 4 to 6 C 8), with pale white tomentum. Smell and taste: none (Figs 4 , 5 ).

Bioluminescence: the whole basidiomata and the mycelia on the PDA emit yellowish-green light and spore print (Fig. 3 b).

Basidiospores (5.2 –) 6.0–7.6 (- 8.4) × 3.0–3.5 (- 4.0) μm, Q = 1.36-2.47 (n = 50), Qm = 1.85, ellipsoid to elongate, hyaline, smooth, amyloid, thin-walled. Basidia 18–25.5 × 4.0–5.8 μm, subclavate to clavate, 4 - spored, hyaline, inamyloid, thin-walled. Cheilocystidia 18.8–27.6 × 6.5–9.2 μm, thick-walled, irregularly clavate to furcate, occasionally subcoralloid, hyaline, inamyloid. Pleurocystidia absent. Pileipellis hymeniform, pyriform, globose, subglobose or clavate, thin-walled, hyaline and inamyloid. Caulocystidia 26–40.4 × 6.8–12.4 μm, thin-walled, subclavate to furcate or coralliform, inamyloid. Clamp connections are present in all tissues (Figs 4 , 5 ).

Distribution

Asia ( China and Malaysia) and Australasia ( New Caledonia and Papua New Guinea).

Ecology

Gregarious to caespitose on decaying wood.

Notes

Chew et al. (2015) transferred M. pruinosoviscida to the Roridomyces genus, based on morphological and molecular data (LSU) from specimens collected in Peninsular Malaysia. Desjardin et al. (2008) found R. pruinosoviscidus (as M. pruinosoviscidus ) predominantly in Australasia and South and Southern Asia. Morphological features of our specimen that correspond to R. pruinosoviscidus include a parabolic to convex or pulvinate, white pileus; a viscid to glutinous stipe; clavate to bifid cheilocystidia; and a hymeniform pileipellis ( Chew et al. 2015). In the phylogenetic analysis of the multigene (Fig. 6 a ), however, only LSU sequences from the previous species, R. pruinosoviscidus ( ACL 273 and ACL 300 ), are available; additional collections and resequencing of type specimens are needed and should be appropriately documented.

Figures

Figure 3. Cultures of bioluminescent fungi on PDA in daylight (a 1 and b 1) and dark conditions (a 2 and b 2) after seven days incubation at 25–28 ℃. a Mycena semivestipes (GMBCC 1066); b Roridomyces pruinosoviscidus (GMBCC 1068). Scale bars = 10 mm.

Figure 4. Basidiomata of Roridomyces pruinosoviscidus (GMB 1054). a, c, e Photographs in daylight in a room and the field; b, d Bioluminescent photographs in complete darkness. Scale bars: 10 mm.

Figure 5. Microscopic characteristics of Roridomyces pruinosoviscidus. a Spores; b Basidia; c Cheilocystidia; d Pileipellis elements; e Stipitipellis with caulocystidia. Scale bars = 10 µm.

Figure 6 a. Roridomyces clade;