Pauqueba bonita James, Bartz & Brown, 2023

James, Samuel W., Bartz, Marie L. C. & Brown, George G., 2023, New Ocnerodrilidae genera, species and records from Brazil (Annelida: Crassiclitellata), Zootaxa 5255 (1), pp. 235-269 : 256-258

Pauqueba bonita James, Bartz & Brown , sp. nov. ( Fig. 13 a-d)

Holotype. BRBADNA0326, one adult, Serra Bonita Private Reserve ( RPPN Serra Bonita), Camac „, Bahia state, Brazil, S15.38485° W39.57453°, 908 m asl., in bromeliad leaf axils of primary forest; 11 November 2014, M.L.C. Bartz, H. Nadolny, E. da Silva and G.G. Brown, colls.

Paratypes. BRBADNA0327, 3 late juveniles, same data as holotype.

Other material. BRBADNA0328A-C, 3 late juveniles, same data as holotype, one individual in each vial.

Etymology. The species is named for Serra Bonita, the type locality, and because in life, this species is a brilliantly colored deep blue-violet.

Description. Dimensions 61-67 mm by 3.3-3.4 mm at segment x, 3.3 mm at clitellum, 3.1 mm at xxx, body slightly ovate flattened, segments 151-170. Setae widely paired or unpaired; first setae in iii, setal formula AA:AB: BC:CD:DD = 2.5:1:3.5:3.3:6 at x, 2.7:1:3.5:2.7:6 at xxx. Prostomium broad epilobous open, but furrow 1/2 missing so could be tanylobous from length of prostomial grooves; segments lacking secondary annulations. Deep dorsal chestnut brown pigment in alcohol preservation, bright blue-purple in life; ventral face of head segments brown, AA zone of ventrum otherwise unpigmented. No dorsal pores, spermathecal pores 8/ 9 in A, wide slits. Ovipores in xiv in B midway between equator and 13/14; combined secondary male and prostatic pores equatorial in xix; primary male and prostatic pores within copulatory pouch; male pores at anterior ends of very short seminal grooves within the pouch; prostatic pores on small papillae visible through secondary pore openings. Setae AB present in xvii, xviii, lacking in xix. Clitellum on xiv-xix, saddle to A; genital markings indistinct, paired in AB of xviii ( Fig. 13a, b ).

Septa 6/7-12/13 slightly muscular, septa and entire peritoneum in anterior segments with red pigment. Alimentary canal with long esophagus in vi, shorter and more iridescent esophagus in vii, neither comprising a gizzard; esophagus valvular in xii, intestinal origin in xiii, laterally pouched in xiii-xvi or xvii; no typhlosole. Calciferous glands reniform, paired in ix and x ( Fig. 13c ), with a honeycomb internal structure leading into wide tubes; glands of ix with blood vessel from anterior end to extra-esophageal vessel, and blood vessel from posterior end of gland to supra-esophageal vessel; glands of x with blood vessels through duct of gland. Gland attachment dorso-lateral face of esophagus. Holonephric, first nephridia in segment vi, in intestinal segments small, confined to BC, tubular throughout.

Vascular system with ventral trunk, single dorsal trunk, lateral vessels in v-ix, esophageal hearts in x-xi. Supraesophageal vessel in ix-xi.

Fan-shaped ovaries, with funnels in xiii; paired large spermathecae in ix, consisting of duct with ectal half muscular, internally fluted, bearing sperm masses, duct externally marked off from ampulla, internally the fluting stops abruptly.Ampulla a flat ovate sac tightly bound to lateral body wall by short muscle or connective tissue fibers ( Fig. 13c ).

Male sexual system holandric, testes and funnels free in x, xi; vas deferens very small, superficial on body wall from 10/11 to xviii where it enters body wall anterior of copulatory pouch in xix; seminal vesicles acinous in xi, xii. Slender tubular prostates coiled twice; once in a tight spiral and secondly the primary coil is wrapped around a band of mesentery with blood vessels, thus very long glands are densely packed under intestine within xv-xx ( Fig. 13d ). Prostatic ducts muscular, widening ectally to union with low muscular thickening marking the intraparietal copulatory pouch in xix. Transverse muscle fibers in xviii-xx crossing male field area.

Remarks. Compared to Paqueba uiraussu sp. nov. it has a saddle-shaped clitellum (vs. annular), combined secondary male and prostatic pores (vs. separate within short, T-shaped seminal grooves and only the prostatic pores secondary), genital markings in xviii (vs. none), first nephridia in vi (vs. iv), intraparietal copulatory pouch with male and prostatic pores (vs. coelomic with only prostatic pores). DNA barcodes are in the Supplemental Information as: BRBADNA0326 (holotype), BRBADNA0327(paratype), BRBADNA0328A, BRBADNA0328B, BRBADNA0328C. Both ASAP and the K2P 14% MOTU separation criteria place this as a species-level lineage.

Figures

FIGURE 13. Pauqueba bonita sp. nov. A. Ventral view, scale bar 2 mm; B. Detail of male field with male porophore (mp); C. Internal view with calciferous gland (cg), spermatheca (s); D. folded prostate glands p.