Duganella lactea, Lu & Deng & Liu & Wang & Yang & Xu, 2020

Lu, Huibin, Deng, Tongchu, Liu, Feifei, Wang, Yonghong, Yang, Xunan & Xu, Meiying, 2020, DUganella laCTea sp. nov., DUganella gUangzHOUensis sp. nov., DUganella flavida sp. nov. and Massilia rivUli sp. nov., isolated from a subtropical stream in PR China and proposal to reclassify DUganella ginsengisOli as Massilia ginsengisOli comb. nov., International Journal of Systematic and Evolutionary Microbiology 70 (8), pp. 4822-4830 : 4827

DESCRIPTION OF DUGANELLA LACTEA SP. NOV.

Duganella lactea (lac’te.a. L. fem. adj. lactea milky, referring to the morphology and colour of colonies).

Cells are Gram-stain-negative, facultative anaerobic, rod-shaped, motile with several flagella, 2.1–2.7 µm long and 0.7–0.9 µm wide. Colonies are creamy and convex. Growth occurs at 4–34 °C (optimum, 24 °C), pH 4.0–10.5 (optimum, pH 7.0–7.5) and 0–1.5% NaCl (w/v; optimum, 0–0.5%). Positive for oxidase, catalase, urease and nitrate reduction, but negative for H 2 S production, phenylalanine deaminase, VP test, methyl red test and indole production. Hydrolyses Tweens (20, 40, 60 and 80) and aesculin, but not casein or starch. Utilizes maltose monohydrate, lactose monohydrate, D-glucose, sucrose and L-arabinose, but not trehalose dihydrate, starch, glycerol, mannitol, D-sorbitol, L-glutamic acid, L-valine, glycine, L-serine,L-aspartic acid, sodium pyruvate, sodium oxalate, sodium formate dihydrate or disodium fumarate. Negative for esterase (C4), cystine arylamidase, trypsin, α -chymotrypsin, β -glucuronidase, α -glucosidase, α -mannosidase and α -fucosidase; positive for alkaline phosphatase, leucine arylamidase, valine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, α -galactosidase, β -galactosidase, β -glucosidase and N - acetylβ -glucosaminidase. The major fatty acids are C 16:1 ω 7 c, C 16:0, C 12:0, C 10:0 3OH and C 18:1 ω7 c. The polar lipids include phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. The G+C content of genomic DNA is 63.3–63.8%.

The type strain, FT50WT (=GDMCC 1.1674 T =KACC 21466 T), was isolated from a subtropical stream in PR China. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and the draft genome of strain FT50WT are MN865818 and WWCP00000000, respectively.