Melanoleuca dirensis F. Nawaz, S. Jabeen & A.N. Khalid, 2017

Nawaz, Farzana, Jabeen, Sana & Khalid, Abdul Nasir, 2017, New and noteworthy Melanoleuca (Pluteaceae) from Pakistan, Phytotaxa 311 (2), pp. 175-184 : 177-180

Melanoleuca dirensis F. Nawaz, S. Jabeen & A.N. Khalid sp. nov.

Figure 2 : A–D; Figure 3 : A–E; Figure 5 : A

MycoBank no.:—MB 820543

Etymology:—the specific epithet refers to the type locality, Dir Upper district.

Diagnosis:—differing from Melanoleuca cinereifolia (Bon) Bon in having white lamellae, small ellipsoid basidiospores, similar cheilocystidia and pleurocystidia without crystals, and a cutis type pileipellis.

Holotype:— PAKISTAN. Khyber Pakhtunkhwa province: Dir Upper district, Kumrat Valley, 2401 m asl, growing on rich loamy soil near Panjkora river , 2 September 2015, Abdul Nasir Khalid and Farzana Nawaz FS 107 ( LAH35159 !; GenBank KU575243 ).

Description:—Basidiomata tricholomatoid, medium. Pileus 42–57 mm diam., convex, obtusely umbonate, margin straight, smooth, glabrous, dry; occasionally cracked, light pink (5P9/4) to yellowish (5Y8/4) becoming grey (7.5Y6/2) to greenish (7.5Y6/4) or dark grey (7.5Y3/2). Lamellae free, crowded, toothed, yellowish (7.5Y9/2) to greenish grey (10Y6/2), with lamellulae of 3 lengths. Stipe 45–67 × 10 mm, eccentric, subequal, subbulbous, surface dry, pruinose, yellowish brown (2.5Y6/4) fibrillose context, becoming white towards base, hollow. Basal mycelium white (7.5Y9/2), abundant.

Basidiospores [40/2/1], (5.4‒) 6‒9.4 (‒10.8) × (3.6‒) 4‒6.8 (‒7.8) μm, [avl × avw = 7.9 × 6 µm, Q = 1.31], ellipsoid, warty, thin-walled, hyaline in 5% KOH, amyloid warts, apiculus present. Basidia 12.7‒32.3 × 6.1‒11.4 μm, [avl × avw = 24.55 × 8.87 μm], clavate, 2‒4 spored, thin-walled, hyaline; sterigmata up to 5.7 μm long. Cheilocystidia 42.9–84 × 8.9–19.7 μm, [avl × avw = 65 × 13.4 µm], fusoid-ventricose, leptocystidia. Pleurocystidia similar to cheilocystidia. Pileipellis a cutis of cylindrical hyphae 6.8–16.8 μm in diameter (av= 11 µm), thin-walled, septate, branched, hyaline. Stipitipellis composed of cylindrical hyphae 4.7–17.2 μm in diam. (av = 11.2 µm), hyaline, septate, branched. Clamp connections absent.

Habitat:—Moist temperate coniferous forests.

Additional specimens examined: PAKISTAN. Khyber Pakhtunkhwa province: Dir Upper district, Kumrat Valley, 2401 m asl, growing on rich loamy soil near Panjkora River , 2 September 2015, Abdul Nasir Khalid and Farzana Nawaz FS 24 ( LAH35160 !; GenBank KU556797 ) .

Figures

FIGURE 2. Basidiomata of Melanoleuca dirensis. A & B. LAH35160 C & D. LAH35159 E & F. M. graminicola LAH35059. Bars: A–D = 1.2 cm, E & F = 0.8 cm. Photographed by Sana Jabeen and Abdul Nasir Khalid.

FIGURE 3. Anatomy of Melanoleuca dirensis. A–E. LAH35159.A. Basidiospores; B. Cystidia; C. Basidia; D. Pileipellis; E. Stipitipellis. Bars:A = 4.7 µm; B = 12 µm, C = 9 µm; D & E = 10.5 µm. Drawing by Sana Jabeen.

FIGURE 5. Scanning electron microscope images of basidiospores. A. Melanoleuca dirensis (LAH35159); B. M. graminicola (LAH35059). Photographed by Abdullah Jan.