Trichoderma mangshanicum K. Chen & W.Y. Zhuang, 2017

Chen, Kai & Zhuang, Wen-Ying, 2017, Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade, Phytotaxa 312 (1), pp. 28-46 : 36-39

Trichoderma mangshanicum K. Chen & W.Y. Zhuang , sp. nov. Fig 5

Fungal Names: FN570281.

Etymology: The specific epithet refers to the type locality.

Diagnosis. Colony growing moderate on CMD and PDA, slow on SNA 25 °C. Conidiophores trichoderma-like, phialides narrowly lageniform, conidia green, globose to subglobose, less commonly ellipsoid.

On CMD after 72 h colony radius 30–31 mm and mycelium covering the plate after 7 d at 25 °C. Colony hyaline, radial, finely zonate, aerial hyphae numerous, forming several concentric rings. Conidiation noted after 3 d, effuse on aerial hyphae, appearing in 5–6 concentric rings. No chlamydospores observed. No distinct odor, no diffusing pigment observed.

On PDA after 72 h colony radius 28 mm and mycelium covering the plate after 7 d at 25 °C. Colony similar to CMD, but aerial hyphae more abundant. Conidiation noted after 2 d, effused on aerial hyphae, numerous. Conidiophores symmetrical, trichoderma-like, comprising a conspicuous main axis with short side branches or phialides, branches paired or unpaired, rebranching 1–2 times. Phialides typically paired, less commonly solitary or in whorls of 3–4, narrowly lageniform, (8.1–)10.0–14.4(–18.1) × 2.2–3.3 μm, l/ w 3.0 –6.0, 1.4–2.8 μm wide at the base (n = 40). Conidia green, with 1 larger or many minute guttules, globose to subglobose, less commonly ellipsoid, 2.8–3.9(–4.6) × 2.8–3.6 μm, l/ w 1.0 –1.4 (n=40). No chlamydospores observed. No distinct odor, no diffusing pigment observed.

On SNA after 72 h colony radius 9–11 mm and mycelium covering the plate after 20 d at 25 °C. Colony hyaline, loose, margin irregular and not well defined, aerial hyphae inconspicuous. Conidiation noted after 7 d, on short erect conidiophores or aerial hyphae. No chlamydospores observed. No distinct odor, no diffusing pigment observed.

Strains examined: CHINA. Hunan: Chenzhou, Mangshan , elev. 1703 m, isolated from soil, 13 November 2015, K. Chen TC860 (holotype HMAS 248810 , ex-type culture CGMCC 3.18324 ). ibid., K. Chen TC862 ( HMAS 248811 ) ; K. Chen TC863 ( HMAS 248812 ) .

Notes: Trichoderma mangshanicum is closely related to T. pararogersonii ( Fig 1 ), but the latter species differs in colony morphology, conidial shape and size (more elongate and slightly larger), and much slow growth rate on SNA (9–11 mm vs. 26–27 mm, 25 °C, 72 h, Jaklitsch & Voglmayr 2015). They show also divergent sequence data of all DNA markers tested in this study.

Figures

FIGURE 1. One of the most parsimonious trees based on the analysis of the combined RPB2 and TEF1 matrix, showing phylogenetic positions of the new species. From left to right: MPBP above 50%, MLBP above 70%, and BIPP above 90% are provided at the nodes.

FIGURE 5. Trichoderma mangshanicum (HMAS 248810). A–C. Cultures after 7 d at 25 °C (A. CMD; B. PDA; C. SNA); D–M. Conidiophores and phialides (PDA, 3 d); N–O. Conidia (PDA, 3 d). Scale bars:A–C = 20 mm; D–K = 10 μm; L–O = 5 μm.