Metaphire, Sims & Easton, 1972

Nguyen, Tung T., Nguyen, Nam Q., Lam, Dang H. & Nguyen, Anh D., 2020, Six new species of the genus Metaphire Sims & Easton, 1972 (Annelida: Oligochaeta: Megascolecidae) from southeastern Vietnam, Raffles Bulletin of Zoology 68, pp. 220-236 : 234-235

Key to the species of Metaphire in southeastern Vietnam

1. Male pores in xix ....................................................................2

– Male pores in xviii ..................................................................3

2. Spermathecal pores paired lateroventrally in 5/6/7/8/9. Clitellum normal, ring-shaped .............. M. anomala ( Michaelsen, 1907)

– Spermathecal pores paired lateroventrally in 5/6/7/8. Clitellum saddle-shaped ( Fig. 6 ) ......................... M. setosa , new species

3. Genital markings present in the male region .........................4

– Genital markings absent in the male region.........................12

4. Intestinal caeca manicate .......................................................... .................................... M. pacseana ( Thai & Samphon, 1988)

– Intestinal caeca simple ............................................................5

5. Spermathecal pores in 5/6/7/8/9 .............................................6

– Spermathecal pores in 6/7/8/9 ................................................9

6. Genital markings in intersegment 19/20 and subsequent intersegments. Setal penis present ............................................ .................................. M. malayanoides Nguyen & Lam, 2017

– Genital markings present in segments. No setal penis ..........7

7. Only one pair of genital markings in xviii. Septum 8/9 absent .................. M. grandiverticulata Nguyen & Lam, 2017

– No genital markings in xviii. Septum 8/9 present ................8

8. Spermathecal pores located dorsally. Four to eight pairs of genital markings in xvii, xix, and subsequent segments ( Fig. 5 )................................................. M. songbeensis , new species

– Spermathecal pores located lateroventrally. Two pairs of genital markings in xvii and xix .......... M. posthuma ( Vaillant, 1868)

9. Spermathecal pores located dorsally. Genital markings present in xvii and xix ( Fig. 2 )........................... M. haui , new species

– Spermathecal pores located not dorsally. Genital markings present in intersegments 17/18 and 18/19............................10

10. Male region strongly concave.............. M. bahli ( Gates, 1945)

– Male region not concave. Genital markings disc-shaped ....11

11. The openings of copulatory pouches close to ventromedial line. Septum 10/11 absent ............ M. saigonensis ( Omodeo, 1957)

– The openings of copulatory pouches not close to ventromedial line. Septum 10/11 present ............. M. peguana ( Rosa, 1890)

12. Intestinal caeca manicate .....................................................13

– Intestinal caeca simple ..........................................................14

13. Spermathecal pores in 5/6/7/8/9. First dorsal pore in 11/12. Seminal vesicles connected ( Fig. 3 ) ......................................... ....................................................... M. bariaensis , new species

– Spermathecal pores in 7/8/9. First dorsal pore in 10/11. Seminal vesicles separated ................... M. californica ( Kinberg, 1867)

14. Spermathecal pore starting from 6/7 ....................................15

– Spermathecal pore starting from 7/8 ....................................17

15. Three spermathecal pores in 6/7/8/9......................................... ........................................................ M. houlleti ( Perrier, 1872)

– Two spermathecal pores in 6/7/8..........................................16

16. First dorsal pore in 12/13. Accessory glands present, ending at spermathecal ducts ........... M. guillelmi ( Michaelsen, 1895)

– First dorsal pore in 11/12. Accessory glands absent ( Fig. 4 ).. ..................................................... M. planatoides , new species

17. Two spermathecal pores in 7/8/9. No setal penis ( Fig. 7 ) ...... .................................................... M. houlletoides , new species

– Only one pair of spermathecal pores in 7/8. Setal penis present........................ M. xuanlocensis Nguyen & Lam, 2017

Figures

Fig. 6. Metaphire setosa, new species, holotype (CTU-EW.179.h01). A1, A2, ventral side of the male region; B1, B2, dorsal side of spermathecal region; C1, C2, intestinal caecum; D1, D2, right prostate gland; E1, E2, left spermathecae; F, testis sacs and seminal vesicles; G, male copulatory pouch, transverse body section; I, ventral side of segments vi–vii. Scale bar = 1 mm.

Fig.5. Metaphire songbeensis, new species, holotype (CTU-EW.176.h01).A1, A2, ventral side of the male region; B1, B2, left spermathecae; C, dorsal side of spermathecal region; D1, D2, intestinal caecum; E, seminal vesicles; F1, F2, right prostate gland. G, genital markings, transverse body section; H, male copulatory pouch, transverse body section. Scale bar = 1 mm.

Fig. 2. Metaphire haui, new species, holotype (CTU-EW.172.h01). A1, A2, ventral side of the male region; B1, B2, left prostate gland; C1, C2, intestinal caecum; D, testis sacs and seminal vesicle; E1, E2, dorsal side of spermathecal region; F1, F2, left spermathecae; G, genital markings, transverse body section; H, male copulatory pouches, transverse body section. Scale bar = 1 mm.

Fig. 3. Metaphire bariaensis, new species, holotype (CTU-EW.169.h01). A1, A2, ventral side of the male region; B1, B2, right prostate gland; C, lateral side of spermathecal region; D1, D2, spermathecae; E, testis sacs and seminal vesicles; F, male copulatory pouch, transverse body section; G1, G2, intestinal caecum. Scale bar = 1 mm.

Fig. 4. Metaphire planatoides, new species, holotype (CTU-EW.171.h01). A, G, ventral side of the male region; B, ventral side of spermathecal region; C, H, spermathecae; D, I, right prostate gland; E, F, intestinal caecum. Scale bar = 1 mm.

Fig. 7. Metaphire houlletoides, new species, holotype. A1, A2, ventral side of the male region; B1, B2, right spermathecae; C1, C2, left prostate gland; D1, D2, ventral side of spermathecal region; E, seminal vesicles; F1, F2, intestinal caecum, right side; G, male copulatory pouch, transverse body section. Scale bar = 1 mm.