Inocybe subhimalayanensis Razzaq, Naseer & Khalid, 2023

Razzaq, Annum, Naseer, Arooj & Khalid, Abdul Nasir, 2023, Inocybe subhimalayanensis (Agaricales, Inocybaceae), a new smooth spored Inocybe species from Pakistan, European Journal of Taxonomy 870, pp. 76-86 : 78-81

Inocybe subhimalayanensis Razzaq, Naseer & Khalid sp. nov. MycoBank MB 845094 Figs 1–2 , Table 1

Diagnosis

The new species differs from Inocybe parvicystis F.J.Rodr. -Campo & Esteve-Rav. by the combination of these characters: prominent umbo, larger spores up to 9.9μm (vs 6.5 μm), hymenal cystidia with non-crystalliferous apex, non-crystalliferous caulocystidia and absence of velipellis.

Etymology

The epithet ‘subhimalayanensis’ is referring to the sub-Himalayan region where the type specimen was collected.

Type material

Holotype

PAKISTAN • Punjab province, Rawalpindi district, Bhurban town; 33.9554° N, 73.4519° E; alt. 1828 m a.s.l.; on soil below Pinus ; 7 Sep. 2020; Annum Razzaq, GB-18; GenBank nos ON810645 (nrITS), ON810652 (LSU); LAH[37437] .

Additional material examined

PAKISTAN • Punjab province, Rawalpindi district, Bhurban town; 33.9554° N, 73.4519° E; alt. 1828 m a.s.l.; on soil below Pinus ; 7 Sep. 2020; Annum Razzaq, GB-26; GenBank nos ON810643 (nrITS), ON810653 (LSU); LAH[37438] • same data as for preceding; 10 Sep. 2021; Annum Razzaq, GC-35; GenBank no ON810644 (nrITS); LAH[37439] • Kashmiri bazar; 10 Sep. 2021; Annum Razzaq, BR-88; GenBank no ON810646 (nrITS); LAH[37440] • same data as for preceding; 4 Sep. 2021; Annum Razzaq, BR-22; GenBank no ON810647 (nrITS); LAH[37441] • same data as for preceding; 12 Sep. 2020; Annum Razzaq, BR-57; GenBank no ON810648 (nrITS); LAH[37442] • Murree; 33.9070° N, 73.3943° E; alt. 2291 m a.s.l.; 18 Aug. 2019; Arooj Naseer AN-36; GenBank no ON911331 (nrITS); LAH[151676] .

Description

Pileus 15–25 mm wide, (sub)campanulate to subumbonate when immature becoming broadly convex or expanded at maturity, with prominent large umbo; margin decurved at young stage later becoming straight, incised to eroded, concolorous, pileus depressed near the umbo; prominent dark brown radial striations at pileal surface extended up to the central umbonate region; color yellowish orange (10YR 7/8) to orange (7.5YR 8/8) when fresh becoming yellowish brown (7.5YR 6/8) to brown hue (7.5YR 5/8) when old. Hymenophore regular, close, adnexed, ventricose, whitish at first becoming brownish with age; edge entire. Stipe 21–35 × 2–4 mm, cylindrical or curved, fine whitish tomentum when young, later becoming longitudinally striate or glabrous, yellowish orange (10YR 7/8) to brown (7.5YR 5/8) colored. Annulus and Volva absent. Context thin. Habit pluteoid. Odor unspecific. Taste not recorded.

Basidiospores [100/3/3] (8.4‒)8.6‒12.2(‒12.6) × (4.9‒)5.1‒7.1(‒7.3) µm, avl × avw = 9.9 × 5.9 µm, Q = 1.62‒1.73, avQ = 1.69, smooth, (sub)amygdaloid with rounded base and rounded to acute apex in side view, oblong to ovoid with rounded to (sub)acute apex in frontal view, often with explicit suprahilar depression, prominent hilar appendix, moderately thick-walled, guttulated, pale to dark brown in 5% KOH and water. Basidia (24.8‒)26.2–35.2(‒38.7) × (12.2‒)13.3–19.2(‒19.7) µm, avl × avw = 29.8 × 8.4 µm, thin-walled, hyaline in 5% KOH, tetrasterigmate, broadly clavate, content present. Pleurocystidia (35.2‒)37.2–49.4(‒49.6) × (7.7‒)8.1–12.6(‒14.2) µm, avl × avw = 42.6 × 16.6 µm, clavate or (sub)- clavate to broadly clavate or (sub)cylindrical or fusiform, metuloidal,thick walled, at apex generally wide, apex non crystalliferous. Cheilocystidia (28.3‒)32.3–48.3(‒49.4) × (13.1‒)14.2–22.4(‒23.6) µm, avl × avw = 37.2 × 16.9 µm, similar to pleurocystidia in size and shape. Pileipellis a cutis of parallel hyphae 3.5–11.5 µm wide, subcutis with wider and paler to colorless hyphae. Stipitipellis a cutis of parallel hyphae, 1.73‒16.2 µm thick (av = 7.6 µm), septate, branched. Caulocystidia only near the apex of the stipe, (56.2‒)58.4–97.8(‒101.1) × (17.7‒)19.2–35.7(‒37.6) µm, avl × avw = 78.1 × 23.4 µm, long and narrow subcylindrical to (sub)utriform; intermixed.

Habitat

Found from nutrient-rich soil, under Pinus wallichiana (Pinaceae) .

Distribution

This species is reported for the first time from sub-Himalayan forests of Punjab, Pakistan.

Figures

Fig. 1. Macromorphological characters of Inocybe subhimalayanensis Razzaq, Naseer & Khalid sp. nov. A. LAH37438. B. LAH37439. C‒D. LAH37437 (holotype).

Fig. 2. Inocybe subhimalayanensis Razzaq, Naseer & Khalid sp. nov. holotype (LAH37437). A. Basidiospores. B. Pleurocystidia. C. Cheilocystidia. D. Basidia. E. Caulocystidia. F. Stipitipellis. G. Pileipellis.