Neotrops poguazu Grismado and Ramírez, 2013

Lipke, Elisabeth & Michalik, Peter, 2015, Evolutionary Morphology Of The Primary Male Reproductive System And Spermatozoa Of Goblin Spiders (Oonopidae; Araneae), Bulletin of the American Museum of Natural History 2015 (396), pp. 1-72 : 12

Neotrops poguazu Grismado and Ramírez, 2013

SPERM TRANSFER FORM ( fig. 11 ): Ovalshaped synspermia (,10 Mm), comprising four sperm ( fig. 11A ). In the periphery of the sperm conjugate, numerous membranes are visible ( fig. 11B , arrow). Synspermia surrounded by a thin (, 80 nm) secretion sheath ( fig. 11B, C ); cytoplasm electron dense within sperm conjugates of the ejaculatory duct ( fig. 11D ), some mitochondria present.

SPERMATOZOA ( fig. 11 ): Acrosomal complex: AV conical with wide subacrosomal space ( figs. 11A, C ), proximally sunken into the anterior pole of prcN ( fig. 11C ). AF originates from subacrosomal space, extends into NC, but clearly ends before axonemal base. Nucleus: prcN deeply indented at its anterior pole (bowl shaped), irregularly shaped with irregularly condensed chromatin ( fig. 11A, B ). peN short, flattened, ( fig. 11D ). NC peripheral ( fig. 11 A–C). Axoneme: proximal centriole longer than distal centriole, 9+3 microtubular pattern.

Note on Spermiogenesis ( fig. 12 ): All stages of spermiogenesis present in the testis. Spermatids of the same developmental stage arranged in cysts. Early spermatids characterized by a large, oval nucleus surrounded by a manchette of microtubules and small AV ( fig. 12A inset) that is separated from the nucleus by a distinct electron-dense border ( fig. 12A ). The AF originates from the subacrosomal space (12C). The chromatin begins condensing in the center of the nucleus and appears fibrillar ( fig. 12A, B ). The small implantation fossa is irregularly shaped in early spermatids ( fig. 12B, B inset). There is always a small amount of electron-dense, granular material associated with the proximal centriole ( fig. 12D ). A collar of electron-dense, platelike secretions surrounds the base of the Ax ( fig. 12E ). At the end of spermiogenesis late spermatids that remained connected via cellular bridges start to fuse, finally resulting in synspermia.

Figures

Fig. 11. Characteristics of synspermia and spermatozoa of Neotrops poguazu. A: Sperm conjugates are surrounded by a thin secretion sheath. B: The chromatin is irregularly condensed. C: The AF extends toward the nucleus and projects into the periphery of the nucleus, surrounded by the NC. D: Within the ejaculatory duct sperm conjugates further differentiate. This includes further condensation of cytoplasm while peripheral constrictions recess.

Fig. 12. Characteristics of spermiogenesis of Neotrops poguazu. A: The small AV is bordered from the nucleus by a distinct electron-dense plate (magnified in inset). B: The chromatin starts condensation irregularly and appears fibrillar in midspermatids (inset). At the posterior pole of the nucleus, an irregularly shaped IF is formed through migration of the two centrioles toward the posterior pole of the nucleus. C: During further development, the AV enlarges and sinks deeply into the anterior portion of the nucleus, while the subacrosomal space widens. D: A small amount of secretion is attached to the proximal centriole within the wide IF. Note the elongated proximal centriole, which is arranged rectangular to the distal centriole. E: Distinct electron-dense lamellae are arranged around the base of the Ax (arrows).