Neoxyphinus termitophilus ( Bristowe, 1938 )

Lipke, Elisabeth & Michalik, Peter, 2015, Evolutionary Morphology Of The Primary Male Reproductive System And Spermatozoa Of Goblin Spiders (Oonopidae; Araneae), Bulletin of the American Museum of Natural History 2015 (396), pp. 1-72 : 28-32

publication ID 10.1206/906.1


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Neoxyphinus termitophilus ( Bristowe, 1938 )


Neoxyphinus termitophilus ( Bristowe, 1938)

SPERM TRANSFER FORM ( figs. 18 View Fig , 19 View Fig ): Large (,25 Mm), oval-shaped synspermia (within the ejaculatory duct) comprising four sperm ( fig. 18 View Fig ). Synspermia, located in the deferent ducts, are bottle shaped ( fig. 18 View Fig ). The bottleneck contains the acrosomal vacuoles, the short and helically contorted precentriolar parts of the nucleus and the base of the axonemes, as well as postcentriolar elongations of the nucleus, whereas the voluminous bulge is filled with the main parts of axonemes and postcentriolar elongations of nucleus that coil around each other. Numerous mitochondria are located in the periphery of the sperm conjugate ( fig. 19A, B View Fig ). A very thin and loosely appearing secretion sheath (, 30 nm), which is produced

in the ejaculatory duct, surrounds the sperm conjugates ( fig. 19 View Fig A–C).

SPERMATOZOA ( figs. 18 View Fig , 19 View Fig ): Acrosomal complex: AV short (,1.2 Mm) and cylindrical

( fig. 18 View Fig ). AF originates from the subacrosomal space and extends into the nuclear canal but ends clearly ends before the axonemal base. Nucleus: prcN short (,10.1 Mm), helically contorted with medium-sized implantation fossa that is filled with numerous mitochondria ( fig. 19A View Fig ), several spherical secretions, proximal and distal centriole, as well as the base of the Ax. peN extremely elongated (,171.8 Mm), oval in cross sections with distinct projection ( fig. 19A, E View Fig ). NC located within a distinct projection, twists around the peN ( figs. 18 View Fig , 19A, E View Fig ) but is empty for the most part ( fig. 19D View Fig ). Axoneme: extremely long (,291.5 Mm); centrioles arranged in tandem position; 9+3 axonemal pattern ( fig. 19B, E View Fig ).

NOTES ON SPERMIOGENESIS: Within the testis all stages of spermiogenesis are present, developing spermatids are arranges in cysts. Early spermatids are characterized by a large, oval nucleus that is surrounded by a manchette of microtubules. Mid spermatids are characterized by a small, cylindrical AV ( fig. 20A, B View Fig ) that is partly sunken into the anterior pole of the nucleus and accompanied by a small amount of electron-dense material ( fig. 20B View Fig ). Further development includes condensation of chromatin that appears fibrillar in mid spermatids ( fig. 20C, D View Fig ). Mitochondria are always present within the implantation fossa ( fig. 20D View Fig , F–H). Mid- and late spermatids show a characteristic chromatin-condensation pattern in which a small portion remains homogeneously electron lucent ( fig. 20E, E View Fig inset). The axoneme is twisted around the postcentriolar elongation, thus both cell components seem to interact ( fig. 20F View Fig ). In late spermatids, both centrioles are arranged in tandem position ( fig. 20G View Fig ). The implantation fossa is filled with numerous mitochondria ( figs. 20 View Fig F–H). The two centrioles and the base of the Ax are surrounded by electron-dense secretion droplets and electron-dense secretion plates ( fig. 20H View Fig ). At the end of spermiogenesis four spermatids fuse and form large and voluminous synspermia. During early stages of this sperm conjugation, four mainly individualized spermatids that remained connected to each other via cellular bridges arrange in close association. While the Ax’s, as well as peN’s coil, and form the massive bulge of early bottle-shaped synspermia, the cellular bridges widen posteriorly resulting in a large amount of common cytoplasm. Finally, the common cytoplasm, which contains numerous mitochondria, encases the spermatids secondarily during further differentiation of the sperm conjugate.